Determination of Genetic Diversity in Apodemus mystacinus (Mammalia: Rodentia) based on SSRs
Year 2019,
, 1098 - 1108, 01.06.2019
Gül Olgun Karacan
,
Dilek Beteş
Abstract
The aim of this study is to determine the genetic diversity of species in Rocky mouse, Apodemus mystacinus, using SSR (the simple sequence repeat) loci and to reveal the reasons of this variations, if any. In this study, 69 A. mystacinus samples were studied collected from 18 localities in Turkey. 7 SSR loci were used to determine the genetic diversity of A. mystacinus. As a result of this study, A. mystacinus includes 2 genetic groups that indicate the presence of two subspecies as A. m. mystacinus that is distributed in western Anatolia and A. m. euxinus in eastern Anatolia. This result also supports that one of the micro refugium areas is eastern Turkey and the other western and southern Turkey.
Supporting Institution
Ankara University, Scientific Research Projects Coordination Unit
Project Number
14B0430001
Thanks
This study was supported by Ankara University, Scientific Research Projects Coordination Unit (BAPRO: 14B0430001). We would like to thank to Prof. Dr. Reyhan ÇOLAK and Prof. Dr. Ercüment ÇOLAK for sharing their valuable contributions, equipments and collecting specimens in this study.
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Apodemus mystacinus’un (Mammalia: Rodentia) Genetik Çeşitliliğinin SSR ile Belirlenmesi
Year 2019,
, 1098 - 1108, 01.06.2019
Gül Olgun Karacan
,
Dilek Beteş
Abstract
Bu çalışmanın amacı, SSR (basit dizi tekrarları) lokuslarını kullanarak kayalık faresinin, Apodemus mystacinus, genetik çeşitliliğini belirlemek ve varsa bu çeşitliliğin nedenleri ortaya koymaktır. Bu çalışmada, Türkiye'deki 18 lokaliteden toplanan 69 A. mystacinus örneği çalışılmıştır. A. mystacinus'un genetik çeşitliliğini belirlemek için 7 SSR lokusu kullanılmıştır. Bu çalışmanın sonucuna göre A. mystacinus, iki alttürün varlığını gösteren 2 genetik grup içermektedir; Anadolu’nun batısında A. m. mystacinus ve Anadolu’nun doğusunda A. m. euxinus. Bu sonuç aynı zamanda mikro sığınak alanlarından birinin Türkiye'nin doğusunda, diğerinin ise batısında ve güneyinde olduğunu desteklemektedir.
Project Number
14B0430001
References
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- Gündüz I, Jaarola M, Tez C, Yeniyurt C, Polly PD, Searle JB, 2007. Multigenic and morphometric differentiation of ground squirrels (Spermophilus, Sciuridae, Rodentia) in Turkey, with a description of a new species. Molecular Phylogenetics and Evolution, 43: 3-6.
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- Gür H, 2017. Anadolu diyagonali: Bir biyocoğrafi sınırın anatomisi. Kebikeç, İnsan Bilimleri İçin Kaynak Araştırmaları Dergisi, 43: 177-187.
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Hewitt GM, 2000. The genetic legacy of the Quaternary ice ages. Nature, 405: 907–913.
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- İbiş O, Koepfli K-P, Özcan S, Tez C, 2018. Genetic analysis of Turkish martens: Do two species of the genus Martes occur in Anatolia?. Zoologica Scripta, 47: 390–403.
- Jaarola M, Martınkova N, Gündüz İ, Brunhoff C, Zima J, Nadachowski A. et al., 2004. Molecular phylogeny of the speciose vole genus Microtus (Arvicolinae, Rodentia) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 33: 647–663.
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- Michaux JR, Chevret P, Filippucci MG, Macholán M, 2002. Phylogeny of the genus Apodemus with a special emphasis on the subgenus Sylvaemus using the nuclear IRBP gene and two mitochondrial markers: cytochrome b and 12S rRNA. Molecular Phylogenetics and Evolution, 23: 123–136.
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