Cypermethrin Etken Maddeli Bir İnsektisitin Cucumis sativus L. (Hıyar) Üzerine Morfolojik ve Fizyolojik Etkileri

Abstract

ÇalıĢmada, sera koĢullarında yetiĢtirilen hıyar (Cucumis sativus L.) bitkilerine farklı konsantrasyonlarda (Önerilen doz = 0.4 ml L-1 su, önerilen dozun iki katı (x2) = 0.8 ml L^-1 su ve üç katı (x3) = 1.2 ml L^-1 su) Cypermethrin etken maddeli bir insektisit uygulanmıĢtır. Morfolojik ölçümlerin yanında, çimlenme yüzdeleri, total protein ve prolin miktarları, fotosentetik pigment maddeleri ve endogen hormon içeriği de incelenmiĢtir. Uygulanan insektisitin tohumların çimlenmeleri üzerinde etkili olmadığı görülmüĢtür. Ancak büyüme parametrelerinden kök uzamasını inhibe ettiği belirlenmiĢtir. Fotosentetik pigment maddelerinde klorofil a, b ve toplam klorofil değerlerinde kontrol grubuna göre x3 uygulama grubunda azalma olmuĢ, karotenoid değerlerinde ise artıĢ meydana gelmiĢtir. Protein, prolin, Absisik asit (ABA) ve Indol-3 Asetik asit (IAA) miktarlarında kontrole göre x3 uygulama grubunda artıĢlar olduğu görülmüĢtür. Bu sonuçlara göre; uygulanan insektisitin özellikle yüksek konsantrasyonlarda uygulanması durumunda hıyar bitkilerinde abiyotik stres yarattığı, strese bağlı olarak bitki metabolizmasını, büyümeyi ve geliĢmeyi olumsuz yönde etkilediği ortaya konmuĢtur.

Keywords

• Cucumis sativus L.,cypermethrin,prolin,protein,stres

Morphological and Physiological Effects of the Insecticide with Cypermethrin Active Substance on Cucumis sativus L. (Cucumber)

Abstract

In this study cucumber (Cucumis sativus L.) plants grown in greenhouse conditions were treated with different concentrations (Recommended dose = 0.4 ml L^-1 water, twice the recommended dose (x2) = 0.8 ml L-1 water and three times (x3) = 1.2 ml L^-1 water) Cypermethrin active insecticide. In addition to morphological measurements, germination percentages, total protein and proline quantities, photosynthetic pigment substances and endogen hormone content were also examined. The insecticide applied did not seem to be effective on germination of seeds. However, it was determined that it inhibited root elongation, one of the growth parameters. Chlorophyll a, b and total chlorophyll values decreased in x3 treatment group and increase in carotenoid values compared to the control group. Protein, proline, abscisic acid (ABA) and Indol-3 Acetic acid (IAA) values were found to increase in the x3 treatment group relative to control. According to these results; it has been determined that applied insecticide causes abiotic stress in cucumber plants especially with high concentrations. It has been shown that plant metabolism, growth and development are affected negatively by stress.

Keywords

• Cucumis sativus L.,cypermethrin,proline,protein,stress

References

1. Alia K, Prasad VSK, Pardhasaradhi P, 1994. Effect of zinc on free radicals and proline in Brassica and Cajanus. Phytochemistry, 39, 45-47.
2. Ashraf M, Foolad MR, 2007. Roles of glycine betaine and proline in improving plant abiotic stres resistance. Environmental and Experimental Botany, 59: 206-216.
3. Bashir F, Mahmooduzaffar, Siddiqi TO, Iqbal M, 2007. The antioxidative response system in Glycine max (L.) Merr. exposed to Deltamethrin, a synthetic pyrethroid insecticide. Environmental Pollution, 147(1): 94-100.
4. Bates LS, Waldren RP, Tevre IU, 1973. Rapid determination of free proline for waterstress studies. Plant and Soil, 39, 205-207.
5. Bohnert HJ, Jensen RG, 1996. Strategies for engineering water-stress tolerance in plants. Trends in Biotechnology, 14, 89-97.
6. Bradford M, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry, 72, 248-254.
7. Bragança I, Lemos PC, Barros P, Delerue-Matos C, Domingues VF, 2018. Phytotoxicity of pyrethroid pesticites and its metabolite towards Cucumis sativus. Science of The Total Environment, Apr 1;619-620:685-691, doi: 10.1016/j.scitotenv.2017.11.164.
8. Cramer GR, Lynch J, Lauchli A, Epstein E, 1987. Influx of Na+, K+ and Ca2+ into roots of salt-stressed cotton seedlings: effects of supplemental Ca2+. Plant Physiology, 83: 510-516.

9. Edreva A, 1998. Molecular bases of stres in plants. Bitkilerde Stres Fizyolojisinin Moleküler Temelleri Sempozyumu, 22-26 Haziran, E.Ü. Ziraat Fakültesi, E.Ü. Bilim-Teknoloji Uygulama ve Araştırma Merkezi, Bornova-İzmir, 234 s.
10. Eun SO, Youn HS, Lee Y, 2000. Lead disturbs microtubule organization in the root meristem of Zea mays. Physiologia Plantarum, 110: 357-365.
11. Fayez KA, 2000. Action of photosynthetic Diuron herbicide on cell organelles and biochemical constituents of the leaves of two soybean cultivars. Pesticide Biochemistry and Physiology, 66: 105-115.
12. Fayez KA, Gerken I, Kristen Y, 1995. Ultrastructural responses of root caps to the herbicides chlorsulfuron and metsulfuron methyl, In: Baluska, F., et al. (eds.): Structure and Function of roots. Kluwer Acad. Publ., 277-287.
13. Fidalgo F, Santos I, Salema R, 1993. Effects of deltamethrin on field grown potato plants; Biochemical and ultrastructural aspects. Annals of Botany, 72, 263-267.
14. Flocco CG, Gorranza MP, Carvajal LG, Loewy RM, Rechen de D’Angelo AM, Giulietti AM, 2004. Removal of azinphos methyl by alfaalfa plants (Medicago sativa L.) in a soil-free system. The Science of the Total Environment, 327(1-3): 31-39.
15. Hananya EB, Goldschmidt EE, Goren R, 1976. Ethylene Induced Formation af ABA in Citrus Pell as related to chloroplast transformations. Plant Physiology, 58: 377-399.
16. Hock WK, Day LG, Morley AW, 1991. Farm Chemicals Manual: A Guide to Safe Use and Handling. The Agric. And Vet. Chem. Assoc. Aust, Ltd.
17. Hopkins WG, 1995. Introduction to plant physiology. John Willey and Sons., Inc., USA, 464 p.
18. Hsu SY, Hsu YT, Kao CH, 2003. The effect of polyethylene glycol on proline accumulation in rice leaves. Biologia Plantarum, 46, 73-78.
19. Jones VP, Toscano NC, Johnson MW, Welter SC, Younman RR, 1986. Pesticide effect on plant physiology: integration into a pest management program. Bulletin of the Entomological Society of America, 32, 103-109.
20. Kavi Kishore PB, Sangam S, Amrutha RN, Laxmi PS, Naidu KR, Rao KRSS, Rao S, Reddy KJ, Theriappan P, Sreenivasulu N, 2005. Regulation of proline biosynthesis, degredation, uptake and transport in higher plants: its implications in plant growth and abiotic stres tolerance. Current Science Association, 88, 424-438.
21. Korte F, Kvesitadze G, Ugrekhelidze D, Gordezian M, Khatisashvili G, Buodze O, Zoalishvili G, Coulston F, 2000. Organic toxicants and plants. Ecotoxicology and Environmental Safety, 47: 1-26.
22. Köse N, 1989. Bazı Çökerten Etmenleriyle Kimyasal Savaşım Üzerinde Çalışmalar. Yüksek Lisans Tezi, Bornova-İzmir.
23. Kumar Pandey J, Gopal R, 2011. Laser-Induced chlorophyll fluorescence: A technique for detection of dimethoate effect on chlorophyll content and photosynthetic activity of wheat plant. Journal of Fluorescence, 21: 785-791.
24. La Rosa PC, Hasegawa PM, Rhodes D, Clithero JM, Watad AEA, Bressan RA, 1987. Abscisic acid stimulated osmotic adjustment and its involvement in adaptation of tobacco cells to NaCl. Plant Physiology, 85: 174-181.
25. Lewitt J, 1980. Responses of plants to environmental stresses. 1 : 3-18 Academic press, New York, U.S.A.
26. Liotenberg S, North H, Marion-poll A, 1999. Molecular biology and regulation of abscisic acid biosynthesis in plants. Plant Physiology and Biochemistry, 37 (5): 341-350.
27. Öztürk İ, Tort N, 2004. Fungisit Uygulamasının Domates (Lycopersicon esculentum Mill.) Bitkisi Yapraklarında Bazı Fotosentetik Pigment Maddeleri, Bitkisel Hormonlar ve Protein Miktarları Üzerine Etkisi. C.Ü. Fen-Edebiyat Fakültesi Fen Bilimleri Dergisi, 25: 1, 7-19.
28. Przymusiński R, Rucińska R, Gwóźdź EA, 2004. Increased accumulation of pathogenesis-related proteins in response of lupine roots to various abiotic stres. Environmental and Experimental Botany, 52: 53-61.
29. Rabbinge R, Van Oijen M, 1997. Scenario studies for future agriculture and crop protection. European Journal of Plant Pathology, 103: 197-201.
30. Saladin G, Magne C, Clement C, 2003. Physiolgical stres responses of Vitis vinifera L. to the fungicides fludioxonil and pyrimethonil. Pesticide Biochemistry and Physiology, 77: 125-137.
31. Scarponi L, Vischetti C, Hassan NM, 2002. Effects of Propachlor on the formation of carbonhydrates and proteins in Vicia faba and the response of its defence mechanism. Agrochimica, 156: 165-175.
32. Serraj R, Sinclair TR, 2002. Osmolyte accumulation: can it really help increase crop yield under drought conditions? Plant Cell & Environment, 25, 333-341.
33. Siddiqui ZS, Ahmed S, Stres A, 1999. Effect of systemic fungicides on germination, seedling growth, DNA, RNA and phenolic content of Brassica compestris L., Pakistan Journal of Biological Sciences, 2(4): 1350-1351.
34. Siddiqui ZS, Khan S, 2001. Effect of systemic fungicides and insecticides on absorption spectra, chlorophyll and phenolic contents of Vigna radiata L.Wilczek. Pakistan Journal of Biological Sciences, 4(7): 812-814.
35. Sloan ME, Camper ND, 1986. Effects of alachlor and metolachlor on cucumber seedlings. Environmental and Experimental Botany, 26 (1): 1-7.
36. Spiers JD, Davies FT, He C, Heinz KM, Bogran CE, Starman TW, 2008. Do insecticides affect plant growth and development?– (Research tests foliar insecticides to determine whether applications affect development in gerbera daisies). Greenhouse Grower, February, Vol:2.
37. Steel RGD, Torrie JH, 1980. Principles and Procedures of Statistics. Pp. 403-447. 2nd Ed. McGraw-Hill Inc., New York.
38. Topçuoğlu ŞF, Çakırlar H, 1985. Tuz Stresi Koşulunda Bitkilerde Absisik Asit (ABA) ve Sitokinin Miktarının Değişimi ve Bunun Fizyolojik Olaylar Üzerine Etkileri. Doğa Bilim Dergisi, Seri A2 cilt: 9, sayı: 2.
39. Tort N, Türkyılmaz B, 2003. Physiological effects of captan fungicide on pepper (Capsicum annuum L.) plant. Pakistan Journal of Biological Sciences, 6(24): 2026-2029.
40. Toscano NC, Sances FV, Johnson MW, Lapre LF, 1982. The effect of various pesticides on lettuce physiology and yield. Journal of Economical Entomology, 75, 738-741.
41. Turkyılmaz Unal B, Esiz Dereboylu A, 2015. Some physiological effects of acetamiprid on two cultivars of corn plants. ɸyton, International Journal of Experimental Botany, 84, 144-147.
42. Witham FH, Blaydes DF, Deulin RM, 1971. Experiments in Plant Physiology. Van Nostrand Reinhold Company, New York, 245 p.
43. Xia XJ, Huang YY, Wang L, Huang LF, Yu YL, Zhou YH, Yu JQ, 2006. Pesticides-induced depression of photosynthesis was alleviated by 24-epibrassinolide pretreatment in Cucumis sativus L. Pesticide Biochemistry and Physiology, 86:42-48.
44. Zhu JK, 2002. Salt and drought stres signal transduction in plants. Annual Review of Plant Biology, 53, 247-273.