Temefos’a Maruz Kalan Gökkuşağı Alabalıklarında, Antioksidan Cevaplar

Abstract

Çalışmamızda gökkuşağı alabalıkları temefosun 3 ve 6 mg L-1 dozlarına akut uygulama ile maruz bırakılmıştır. Uygulama sonrasında karaciğer dokusunda antioksidan enzim aktiviteleri (Süperoksitdismutaz (SOD), katalaz (CAT), glutatyon redüktaz (GR), glukoz 6 fosfat dehidrogenaz (G6PD)), malondialdehit seviyesi ve beyin dokusunda asetilkolin esteraz (AChE) aktivitesi değerlendirildi. Karaciğer dokusunda SOD, CAT, GR, G6PD aktivitelerinde ve MDA seviyesinde önemli seviyede değişiklikler belirlendi (P<0.05). Kontrol grubu ile kıyaslandığı zaman tüm doz uygulanan gruplarda AChE aktivitesinin inhibe olduğu tespit edildi (P<0.05). Sonuçlar, temefosun karaciğer dokusunda enzim aktivitelerini inhibe ettiğini ve MDA seviyesini arttırdığını göstermiştir. Bu veriler ışığında sucul ortamlarda temefos toksisitesinin dikkate alınması gerektiğini söyleyebiliriz.

Keywords

• Pestisit,oksidatif stres,asetilkolinesreaz (AChE),gökkuşaği alabalığı

Responses of Antioxidant in Rainbow Trout Exposed to Temephos

Abstract

In this study, rainbow trout was exposed to temephos (TE) during 96h acute toxicity conditions. The toxic compound was given in doses of 3 mg L-1 and 6 mg L-1. Furthermore, we assessment antioxidant enzymes activity (glucose-6-phosphate dehydrogenase (G6PD), catalase (CAT), glutathione reductase (GR), superoxide dismutase (SOD), malondialdehyde (MDA) level in liver and acetylcholinesterase (AChE) in brain. CAT, SOD, GR, G6PD, and MDA in liver were demonstrated significant effects by the treatments (P<0.05). TE exposure resulted in the inhibition of AChE activity, which, compared with the control group, decreased in all doses (P<0.05). The results showed that temephos inhibited liver tissue’s enzyme activities and increased MDA levels. In the light of these data, we can say that temephos toxicity should be taken into consideration in aquatic environments.

Keywords

• Pesticide,oxidative stress,AChE,rainbow trout

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