Investigation of Some Pesticides’ Effects on Activities of Glutathione Reductase and Glutathione S-Transferase Purified from Turkey Liver under in Vitro Conditions

Abstract

Whereas a very small amount of pesticides which are used for elimination of undesirable species

and for a more fertile agriculture reach the target organism, majority of pesticides reach to nontarget organisms. It is

foreseen by our team that the pesticides used for various purposes may negatively affect the glutathione mechanism

of the organisms. However, determination of effective dosage range is the objective of our study subjects. For this

reason, the in vitro effects of widely used pesticides (lambda-cyhalothrin, cypermethrin, chlorpyrifos, dichlorvos,

glyphosate isopropylamine) on the activities of glutathione reductase (GR) and glutathione S-transferase

(GST) which are two important enzymes of the glutathione system have been investigated in this study. It has

been determined that when lambda-cyhalothrin does not affect the GR enzyme purified from the turkey liver,

chlorpyrifos, glyphosate isopropylamine, dichlorvos and cypermethrin cause inhibition. When the effects of

pesticides on GST enzyme purified from turkey liver were investigated in in vitro conditions, it was determined

that all examined pesticides had inhibitory effects. In this context, the potential doses which can create a risk for

live life, of pesticides that are commonly used, have been identified.

Keywords

• Glutathione S-transferase,glutathione reductase; pesticides

Hindi Karaciğerinden Saflaştırılan Glutatyon Redüktaz ve Glutatyon S-Transferaz Aktiviteleri Üzerine Bazı Pestisitlerin Etkilerinin in Vitro Şartlarda İncelenmesi

Abstract

İstenmeyen türlerin ortadan kaldırılması ve daha verimli bir tarım için kulanılan pestisitlerin, çok küçük

bir kısmı hedef organizmaya ulaşırken oldukça büyük bir kısmı da hedef olmayan organizmalara ulaşmaktadır.

Çeşitli amaçlarla kullanılan pestisitlerin söz konusu organizmaların glutatyon mekanizmasını olumsuz şekilde

etkileyebileceği ekibimiz tarafından öngörülmüştür. Ancak etkili dozaj aralığının tespit edilmesi çalışma konumuzun

amacını oluşturmaktadır. Bu nedenle belirtilen çalışmada glutatyon sisteminin iki önemli enzimi olan glutatyon

redüktaz (GR) ve glutatyon S-transferazın (GST) aktiviteleri üzerine oldukça yaygın kullanılan pestisitlerin

(lambda-sihalotrin, sipermetrin, klorpirifos, diklorvos, glifosat izopropilamin) in vitro etkileri araştırılmıştır. Hindi

karaciğerinden saflaştırılan GR enzim aktivitesi üzerine lambda-sihalotrin’nin etki etmediği, klorpirifos, glifosat

izopropilaminin, diklorvos ve sipermetrinin ise inhibisyona sebep olduğu tespit edilmiştir. Hindi karaciğerinden

saflaştırılan GST enzim aktivitesi üzerine söz konusu pestisitlerin etkileri in vitro şartlarda araştırıldığında ise

çalışma kapsamında incelenen tüm pestisitlerin inhibisyon etkisine sahip olduğu belirlenmiştir. Bu kapsamda

yaygın olarak kullanılan zirai ilaçların canlı yaşam için risk oluşturabilecek potansiyel dozajları tespit edilmiştir.

Keywords

• Glutatyon redüktaz,glutatyon S-transferaz,pestisit

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