Rutin Loaded PLGA Nanoparticles; Synthesis by using Different Methods and Characterization

Abstract

Due to the poor solubility of the Rutin molecule, which is a flavonol glycoside found in some plants and especially in citrus fruits and shells, its bioavailability is quite low. In this study, Rutin-loaded-PLGA nanoparticles were synthesized by using different methods such as single emulsion solvent evaporation, nanoprecipitation and salting out, in order to increase the bioavailability of Rutin. These nanoparticles were characterized by their encapsulation efficiency %, average particle size, polydispersity index, Zeta potential, Fourier transform infrared spectroscopy and scanning electron microscopy analysis. In vitro Rutin release studies of NP3 and NP5 were investigated. Rutin release from NP5 reached 41% in 48 hours. It is aimed that the Rutin-loaded nanoparticles synthesized in this work will be suitable candidates for future multidisciplinary studies developed in nanomedicine.

Keywords

• Rutin,PLGA,Emulsion,Nanoprecipitation,Bioavailability

Rutin yüklü PLGA Nanopartiküller; Farklı Yöntemler Kullanılarak Sentezi ve Karakterizasyonu

Abstract

Bazı bitkiler ile özellikle turunçgiller gibi meyvelerde ve kabuklarında bulunan bir flavonol glikozit olan Rutin molekülünün zayıf çözünürlüğü nedeniyle canlı sistemde biyoyararlanımı oldukça düşüktür. Bu çalışmada, Rutin molekülünün biyoyararlanımı arttırmak için tekli emülsiyon solvent buharlaştırma (o/w), nano çöktürme ve tuz etkisi ile çöktürme gibi farklı yöntemler kullanılarak Rutin yüklü PLGA nanopartikülleri sentezlenmiştir. Bu nanopartiküller % enkapsülasyon etkinliği, ortalama parçacık boyutu, çoklu dağılım indisi, Zeta potansiyel, Fourier dönüşümlü kızılötesi spektroskopisi ve taramalı elektron mikroskobu analizleri ile karakterize edilmiştir. NP3 ve NP5’in in vitro ortamda Rutin salımı incelenmiştir. NP5’den % Rutin salımı 48 saatte %41 oranına ulaşmıştır. Yapılan bu çalışmada sentezlenen Rutin-yüklü nanopartiküllerin, ileride nanotıp alanında gelişmiş ve çok yönlü çalışmalar için uygun bir aday olması hedeflenmektedir.

Keywords

• Rutin,PLGA,Emülsiyon,Nanoçöktürme,Biyoyararlanım

Thanks

Yıldız Teknik Üniversitesi Biyomühendislik Bölümü Öğretim Üyesi Yrd. Doç. Dr. Zeynep MUSTAFAEVA AKDESTE ve Doç. Dr. Serap DERMAN’a deneysel çalışmalarım boyunca sağlamış oldukları laboratuvar olanaklarından dolayı teşekkürlerimi sunarım.

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