In Vivo ve In Vitro Koşullar Altında Çeşitli Ön Uygulamaların Türkiye Endemiği Achillea gypsicola Hub.-Mor. Türünün Çimlenmesi Üzerine Etkisi

Abstract

Tıbbi ve aromatik bitkilerde tohum dormansisinin üstesinden gelmek için çeşitli ön uygulamalara karşı artan bir ilgi vardır. Bu çalışma, çeşitli ön uygulamaların Türkiye endemiği Achillea gypsicola Hub.-Mor. türünün in vivo ve in vitro koşullarda çimlenmesi üzerine etkisini belirlemek amacıyla yapılmıştır. Tohumlar, GA3 ve KNO3'ün çeşitli konsantrasyonları ve yedi günlük aralıklarla 91 günlük soğukta katlama gibi üç ön muameleye tabi tutulmuştur. In vivo çimlenme testinde, soğukta katlama ve çeşitli GA3 dozları ile ön muamele edilmiş tohumlarda çimlenme görülmezken, hem in vivo hem de in vitro koşullarda KNO3 uygulamaları çimlenme yüzdesini arttırmıştır. Soğuk katlama, hem in vitro hem de in vivo koşullarda çimlenme üzerinde hiçbir teşvik edici etki göstermemiştir. KNO3 ve GA3'ün in vitro koşullarda kombine uygulanması, tek başlarına uygulamalarından daha yüksek çimlenme yüzdeleri üretmiştir. Tohumların KNO3'te bekletme süresi arttıkça KNO3 ve GA3'ün çimlenme yüzdesi üzerindeki uyarıcı etkisi artmıştır. 48 saat boyunca 200 μM KN03 çözeltisinde bekletilen ve 2 mg L-1 GA3 ile takviye edilmiş MS ortamına ekilen tohumlarda, %100 çimlenme görülmüştür. Sonuç olarak, bu çalışma KNO3'ün GA3 ile birleştirilmiş ön uygulamasının tohum dormansisini kırmada ve endemik Achillea gypsicola Hub.-Mor. türünün çimlenmesini artırmada büyük bir potansiyele sahip olduğunu göstermiştir.

Keywords

• Soğuk katlama,dormansi,giberellik asit,potasyum nitrat,tohum

Project Number

AR-1644

Effect of Various Pretreatments on Germination of Turkish Endemic Achillea gypsicola Hub.-Mor. Species under In Vivo and In Vitro Conditions

Abstract

There has been an increasing interest to overcome seed dormancy in medicinal and aromatic plants using various pretreatments. This study was carried out to determine effect of several pretreatments on germination of Turkish endemic Achillea gypsicola Hub.-Mor. species under in vivo and in vitro conditions. The seeds were subjected to three pretreatments as various concentrations of GA3 and KNO3 and 91day cold stratification, with a seven-day interval. In vivo germination test produced no germination in the seeds pre-treated with cold stratification and various concentrations of GA3, whereas KNO3 treatments both in vivo and in vitro explicitly increased germination percentage. Cold stratification showed no inciting effect on germination both in vitro and in vivo conditions. In vitro combined application of KNO3 and GA3 produced higher germination percentages than single applications. The stimulating effect of KNO3 and GA3 on germination percentage increased as the duration of seed immersing in KNO3 increased. The seeds hold in 200 μM KNO3 for 48 hours and planted on MS medium supplemented with 2 mg L-1 GA3 showed 100% germination. In conclusion, this study suggested that combined pre-treatment of KNO3 with GA3 had a great potential in breaking seed dormancy and increasing germination of Turkish endemic Achillea gypsicola Hub.-Mor. species.

Keywords

• Cold stratification,dormancy,gibberellic acid,potassium nitrate,seed

Supporting Institution

Ordu University

Project Number

AR-1644

Thanks

We are thankful to Scientific Research Projects Unit (BAP) of Ordu University for providing support to this research, with the number of AR-1644 BAP Project.

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