The Evaluation of Antimicrobial and Antibiofilm Activity of Bioactive Compounds Obtained from Aspergillus Sclerotiorum

Year 2019, , 1666 - 1673, 01.09.2019

Ayşe Üstün Ayşenur Yazıcı , Nurcan Albayrak İskender , Serkan Örtücü

https://doi.org/10.21597/jist.512652

Abstract

This study was concerned with the screening of antimicrobial products from fungi collected from soil and evalution of their antibiofilm activity. The isolate having antimicrobial and antibiofilm compounds was characterized by the molecular methods and identified as Aspergillus sclerotiorum. A. sclerotiorum was grown in yeast peptone glucose (YPG) medium and extracellular medium was extraction by 1:1 ethyl acetate. Crude extraction characterized through thin layer chromatography (TLC) on silica gel 60 HF254 and was detected five bands. Agar diffusion and TLC overlay assays were done against Gram-positive (Staphylococcus aureus ATCC 25923, meticilin resistance S. aureus (MRSA) and Enterococcus faecalis ATCC 29212) and Gram-negative bacteria (Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853). The most dense band (Rf:0.42) showed the best inhibition zone on TLC overlay. The results showed that the most dense band can potential source for antimicrobial compound. After the most dense band in silica gel was scraped and dissolved ethyl acetate for minimum inhibitory concentration (MIC) determination and crystal violet assay against S. aureus and MRSA. These results indicate that fungi, A. sclerotiorum, isolated from soil was potential source for antimicrobial and antibiofilm compounds.

Keywords

Antimicrobial, Antibiofilm, Aspergillus sclerotiorum, Fungi, Thin layer chromatography

Aspergillus sclerotıorum’dan Elde Edilen Biyoaktif Bileşiklerin Antimikrobiyal ve Antibiyofilm Aktivitelerinin Değerlendirilmesi

Abstract

Bu çalışma, topraktan izole edilen funguslardan antimikrobiyal bileşiklerin taranmasını ve bu bileşiklerin antibiyofilm aktivitelerinin değerlendirilmesini kapsamaktadır. Antimikrobiyal ve antibiyofilm özellikteki bileşiklere sahip izolat, moleküler olarak karakterize edilmiş ve Aspergillus sclerotiorum olarak tanılanmıştır. A. sclerotiorum maya pepton glikoz (YPG) besiyerinde geliştirilmiş ve ekstrasellüler besiyeri 1:1 oranında etil asetat ile ekstrakte edilmiştir. Ekstraksiyon ürünü ince tabaka kromotografisi (TLC, silica gel 60 HF254) ile karakterize edilmiş ve 5 bant elde edilmiştir. Gram pozitif (Staphylococcus aureus ATCC 25923, metisiline dirençli S. aureus (MRSA), Enterococcus faecalis ATCC 29212) ve Gram negatif (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) bakterilere karşı agar difüzyon ve TLC kapama deneyleri yapılmıştır. Rf değeri 0.42 olan bantın antimikrobiyal aktivite gösterdiği bulunmuştur. Bu bant TLC plaktan geri kazanılarak, minimum inhibitör konsantrasyonu (MIC) belirleme ve kristal viyole testi ile S. aureus ve MRSA’ ya karşı antibiyofilm aktivitesi değerlendirilmiştir. Bu sonuçlar, topraktan izole edilen fungus, A. sclerotiorum'un antimikrobiyal ve antibiyofilm bileşikleri için potansiyel bir kaynak olduğunu göstermektedir.

Keywords

Antimikrobiyal, Antibiyofilm, Aspergillus sclerotiorum, Fungus, İnce tabaka kromotografisi

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