Studies of Anticancer Activity of Beta-carotene, Alpha-tocopherol and Ascorbic acid in SH-SY5Y Neuroblastoma Cells

Year 2019, , 1657 - 1665, 01.09.2019

Ekrem Darendelioğlu

https://doi.org/10.21597/jist.538260

Abstract

Neuroblastoma (NB; SH-SY5Y cells) is important characteristic of the most widespread cancer in children after leukaemia and brain cancer. Discovery of new treatment approaches are required for NB disease. Epidemiological studies have demonstrated that different types of antioxidants are associated with decreased cancer and neurodegenerative diseases. Fruits and vegetables have a plenty source of Beta-carotene (BC), Alpha-tocopherol (vit E) and Ascorbic acid (vit C) that have potent free radicals scavenging actions and inhibitory properties to biochemical oxidation. BC, vit E and vit C increase the intracellular reactive oxygen species (ROS) generation and encourages cell apoptosis. It was found that these antioxidants resulted in an increase in the malondialdehyde (MDA) (marker of lipid peroxidation; LPO) and ROS levels at high concentration suggesting it behaves as a prooxidant in this study. The outcomes obviously indicated that studies of SH-SY5Y with dose-dependent BC, vit E and vit C results in oxidative damage through an increase in ROS and LPO generation and the expression of apoptotic marker caspase-3. These consequences find out additional evidences that these antioxidants could be involved in the process of apoptosis for new therapy in NB cells.

Keywords

Beta carotene, alpha tocopherol, ascorbic acid, apoptosis, SH-SY5Y

SH-SY5Y Nöroblastom Hücrelerinde Beta-karoten, Alfa-tokoferol ve Askorbik asidin Antikanser Etkilerinin Araştırılması

Abstract

Nöroblastom (NB; SH-SY5Y hücreleri), lösemi ve beyin kanserinden sonra özellikle çocuklarda en yaygın görülen kanser türü olduğu için çalışılması tercih edilmiştir. NB için yeni tedavi yaklaşımları gerekmektedir. Bazı ülkelerde yapılan epidemiyolojik çalışmalar, farklı tipte antioksidanların kanser ve nörodejeneratif hastalıkların azalması ile ilişkili olduğunu göstermektedir. Meyve ve sebzeler, biyokimyasal oksidasyonu engelleyici etkileri ve serbest radikalleri yakalama özellikleri olan bol miktarda Beta-karoten (BC), Alfa-tokoferol (vit E) ve Askorbik asit (vit C) içermektedir. BC, vit E ve vit C hücre içi ROS oluşumunu arttırarak apoptozu teşvik edebilmektedir. Bazı çalışmalarda belirtildiği üzere bu antioksidanların yüksek konsantrasyonlarda, LPO ve ROS oluşumunda bir artışa yol açtığı ve prooksidan olarak davrandığı gösterilmektedir. Bu çalışmanın sonucunda elde edilen veriler, BC, vit E ve vit C’nin SH-SY5Y NB hücreleri üzerindeki etkisi doza bağımlı olarak ROS ve LPO üretiminde bir artışa sebep olarak oksidatif hasara ve devamında apoptotik kaspaz-3 proteini ekspresyonunda artışa neden olduğunu açıkça göstermektedir. Elde edilen sonuçlar, bu çalışmada etkisi araştırılan antioksidanların NB hücreleri için apoptoz sürecinde yeni tedavi yaklaşımları açısından değerlendirilebileceğini göstermektedir.

Keywords

Beta-karoten, alfa-tokoferol, askorbik asit, apoptoz, SH-SY5Y

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