Influence of Vitamin E and Organic Selenium Supplementation on Antioxidant Enzymes Activities in Blood and Egg Samples of Laying Hens

Abstract

This experiment was conducted to evaluate the effects of dietary vitamin E (α-tocopherol acetate) and selenium (selenomethionine) and a combination of the two, on the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) and malondialdehyde (MDA) levels in serum, egg yolk and egg white of laying hens. Ninety-six white Lohman laying hens aged of 24 weeks were randomly divided into 4 groups. The groups were fed with the diets that consisted of basal diet (2770 kcal/kg metabolic energy and 17 % crude protein) (Control), basal diet + 250 mg / kg Vit-E (Trial-1), basal diet + 0.9 mg/ kg Se (Trial-2) and basal diet + 250 mg / kg Vit-E + 0.9 mg/ kg Se (Trial-3) respectively for 12 weeks. It was found out that when the treatment and control groups were compared in terms of enzymes in serum and egg samples while SOD, CAT and GSH-Px activities increased (p<0.05) and the levels of MDA decreased in the samples of treatment groups (p<0.05). The results of the present study, is recommended that organic selenium and vitamin E supplemental of laying hen diets, alone or together, increased activites of antioxidan enzymes and decreased MDA concentrations in serum and egg.

Keywords

• Selenium,laying hens,antioxidant enzymes activities,serum,egg

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