Evaluation of Seed Vigour in White Coat French Bean (Phaseolus vulgaris L.) Seed Lots Under Waterlogged or Field Capacity Conditions

Abstract

This study was conducted to determine whether electrical conductivity and early radicle emergence count in ten white coat French bean seed lots were related to seedling emergence in waterlogged or field capacity sowing medium. Electrical conductivity (EC) measırements were performed by soaking seeds at 20 °C over 2, 4, 6, 18 and 24 h. Early radicle emergence percentages (RE) were counted after 24, 28, 32, 36, 40, 44 and 48 hours of germination. Seedling emergence percentages were tested by sowing at waterlogged or field capacity medium. Seedling emergence percentages were lower in waterlogged than in field capacity sowing in all seed lots. The EC and RE values were correlated with seedling emergence. In both waterlogged and field capacity sowings, the highest correlation values were observed in the 18 h (r=0.966; p<0.001; r=0.965, p<0.001) and 24 h of electrical conductivity (r=0.947, r=0.952, p<0.001) and in the 48 h of the RE test (r=0.905, r=0.918, p<0.001) with seedling emergence. Results showed that the seed vigour potential, in terms of emergence potential, of white coated French bean seed lots can be determined either through 18 h and 24 h EC readings or through 48 hours of early radicle emergence count during germination. Changes in seed vigour with respect to waterlogged or field capacity sowings were discussed for French bean seeds.

Keywords

• Early radicle emergence,electrical conductivity,irrigation,seed germination,seedling emergence

References

1. Baskin J.M., Baskin C.C., Li X., 2000. Taxonomy, anatomy and evolution of physical dormancy in seeds. Plant Species Biology, 15: 139–152.
2. De Souza F.D., Marcos-Filho J., 2001. The seed coat as a modulator of seed-environment relationships in Fabaceae. Revista Brasileira de Botanica, 4: 365–375.
3. Ermis S, Karslioglu M, Ozden E, Demir I, 2015. Use of a single radicle emergence count as a vigor test in prediction of seedling emergence potential of leek seed lots. Seed Science and Technology, 43(2): 308-312.
4. Gupta PC, 1993. Seed vigor testing. In: Handbook of seed testing (Ed. Agrawal, P.K.). DAC, Ministry of Agriculture, Govt. of India, New Delhi, 242-249.
5. ISTA, 2017. International Rules for Seed Testing. Bassersdorf, CH- Switzerland.
6. Khajeh-Hosseini M, Nasehzadeh M, Matthews S, 2010. Rate of physiological germination relates to the percentage of normal seedlings in standard germination tests of naturally aged seed lots of oilseed rape. Seed Science and Technology, 38(3): 602-611.
7. Legesse N, Powell AA, 1996. Relationship between the development of seed coat pigmentation, see coat adherence to the cotyledons and the rate of imbibition during the maturation of grain legumes. Seed Science and Technology, 24(1): 23-32.
8. Matthews S, Demir I, Celikkol T, Kenanoglu BB, Mavi K, 2009. Vigour tests for cabbage seeds using electrical conductivity and controlled deterioration to estimate relative emergence in transplant modules. Seed Science and Technology, 37(3): 736-746.

9. Mavi K, 2010. The relationship between seed coat color and seed quality in watermelon Crimson sweet. Hort. Sci. (Prague), 37: 62–69.
10. Nerson H, 2002. Effects of seed maturity, extraction practices and storage duration on germinability in watermelon. Scientia Horticulturae, 93: 245–256.
11. Ozden E, Delialioglu RA, Muhie SH, Demir I, 2017. Prediction of germination of commercially available seed lots by regression models developed by artificial and natural ageing and electrical conductivity in leek. Seed Science and Technology, 45(2): 465-674.
12. Powell AA, De Oliveira MA, Matthews S, 1986. Seed vigor in cultivars of dwarf French bean (Phaseolus vulgaris) in relation to the colour of the testa. Journal of Agricultural Science, 106: 419-425.
13. Usha TS, Dadlani M, 2015. Evaluation of seed vigor in soybean (Glycine max). Legume Research, 38(3): 308-312.