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Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria)

Year 2020, , 723 - 731, 01.06.2020

Samad Khorsandi Alireza Motallebi Azar Fariborz Zaare Nahandi Ali Hatami Sam Mokhtarzadeh

https://doi.org/10.21597/jist.597193
Cited By: 1

Abstract

One of the most critical stages of produced virus free potato plants is rapid and efficient in vitro microtuberization. Plant growth regulators and their concentration is important factor on potato microtuberization. This study was conducted to develop a protocol for rapid microtuber formation for potato micropropagation by different concentrations of 6-Benzylaminopurine (BAP) (0, 1 and 2 mg.l-1) and five concentrations of Putrescine (0, 20, 40, 80 and 160 mg.l-1) with 8% sucrose and 8 g.l-1 agar. Present study was conducted as a factorial experiment based on completely randomized design with three replications in tissue culture laboratory to investigate the effect of different BAP and Putrescine concentrations. Lateral buds of cv. Agria from in vitro shoots were cultured as explants. Cultures were kept in germinator with constant darkness and temperature of 17±2°C. Variance analysis showed that none of the traits were affected by different concentrations of the Putrescine other than weight of the shoots. Also, the interaction effect of PUT×BAP on shoot weight was significant. However, the effect of different levels of BAP was significantly associated with the most trait of microtuberization. In this experiment, the best concentration of BAP for microtuberization was 1 mg.l-1. Also, the appropriate Putrescine level for increasing of microtuberization number and percentage was 80 mg.l-1 but the effect of Putrescine on microtuberization number and percentage was non-significant.

Keywords

Solanum tuberosum in vitro plant growth regulators polyamine

References

  • Akita M, Takayama S, 1994. Induction and development of potato tubers in a jar fermenter. Plant Cell, Tissue and Organ Culture, 36: 177-182.
  • Altindal D, Karadoğan T, 2010. The Effect of Carbon Sources on In Vitro Microtuberization of Potato (Solanum tuberosum L.). Turkish Journal of Field Crops, 15(1): 7-11.
  • Dhital SP, Lim HT, 2012. Microtuberization of Potato (Solanum tuberosum L.) as Influenced by Supplementary Nutrients, Plant Growth Regulators, and In Vitro Culture Conditions, Potato Research, 55 (2): 97-108.
  • Fatima B, Usman M, Ahmad I, Khan IA, 2005. Effect of explant and sucrose on microtuber induction in potato cultivars. International Journal of Agriculture and Biology, 7: 63-66.
  • Gami RA, Parmar SK, Patel PT, Tank CJ, Chauhan RM, Bhadauria HS and Solanki SD, 2013. Microtuberization, minitubers formation and in vitro shoot regeneration from bud sprout of potato (Solanum tuberosum L.) cultivar K. badshah, African Journal of Biotechnology, 12(38): 5640-5647.
  • Garner N, Blake J, 1989. The induction and development of potato microtubers in vitro on media free of growth regulating substances. Annals of Botany, 63:663-674.
  • Gopal J, Minocha JL, Sidhu JS, 1997. Comparative performance of potato crops raised from microtubers induced in dark versus microtubers induced in light. Potato Research, 40: 407-412.
  • Gopal J, Minocha JL, Dhaliwal HS, 1998. Microtuberization in potato (Solanum tuberosum L.). Plant Cell Reports, 16: 794-798.
  • Harmey MA, Crowley MP, Clinch PEM, 1966. The effect of growth regulators on tuberization of cultured stem pieces of Solanum tuberosum. Eur. Potato J, 9: 146-151. Hoque ME, 2010. In Vitro Regeneration Potentiality of Potato under Different Hormonal Combination. World Journal of Agricultural Sciences (WJAS), 6 (6): 660-663.
  • Husain S, Hussain Shah SA, Asghar S, Hussain N, Ali N, Hussain I, Rafiq S, Shah S, Imtiaz M, Ali M, Jala F, Zia-ullah and Rashid M, 2017. Micro-Tuberization of Four Potato (Solanum tuberosum L.) Cultivars Through Tissue Culture. Journal of Botanical Sciences, 6(3): 35-40.
  • Hussey G, Stacey NJ, 1984. Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.). Annals of Botany, 53: 565-578.
  • Imai AA, Qhrmanzadeh R, Azimi J, Janpoor J, 2010. The effect of various of 6-benzylaminopurine (BAP) and sucrose on in vitro potato (Solanum tuberosum L.) microtuber induction. American-Eurasian Journal of Agricultural and Environmental Sciences, 8(4): 457-459.
  • Kakkar RK, Nagar PK, Ahuja PS, Rai VK, 2000. Polyamines and plant morphogenesis. Biologia Plantarum, 43: 1-11.
  • Kazemiani S, Motallebi-Azar AR, Panahandeh J, Mokhtarzadeh S, Ozdemir FA, 2018. Shoot proliferation from potato (Solanum tuberosum cv. Agria) under different concentration of MS include vitamins and BAP medium. Progress in Nutrition, 2018; , 20 (1) 160-166.
  • Khuri S, Moorby J, 1996. Nodal segments or microtubers as explants for in vitro microtuber production of potato. Plant Cell, Tissue and Organ Culture, 45: 215-222.
  • Mader JC, 1995. Polyamines in Solanum tuberosum in vitro: Free and conjugated polyamines in hormone-induced tuberization. Journal of Plant Physiology, 146: 115-120.
  • Martin-Tanguy J, 1997. Conjugated polyamines and reproductive development: biochemical, molecular and physiological approaches. Physiologia Plantarum, 100: 675-688.
  • Momena K, Adeeba R, Mehraj H, Jamal Uddin AFM, Saiful Islam and Rahman L, 2014. In Vitro Microtuberization of Potato (Solanum Tuberosum L.) Cultivar through Sucrose and Growth Regulator. Journal of Bioscience and Agriculture Research, 02 (02): 76-82.
  • Murashige T, Skoog F, 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15: 473−497.
  • Ondo Ovono P, Kevers C, Dommes J, 2009. Tuber formation and development of Dioscorea cayenensis and D. rotundata complex. Effect of Polyamines. In Vitro Cellular & Developmental Biology Plant, 46: 81-88.
  • Pelacho AM, Mingo-Castel AM, 1991. Effects of photoperiod on kinetin-induced tuberization of isolated potato stolons cultured in vitro. American Journal of Potato Research, 86:533-541.
  • Protacio CM, Flores HE, 1992. The role of polyamines in potato tuber formation. In Vitro Cellular & Developmental Biology, 28: 81- 86.
  • Saiprasad GVS, Raghuveer P, 2004. Effect of various polyamines on production of protocorm-like bodies in orchid Dendrobium ‘Sonia’. Scientia Horticulturae, 100: 161-168.
  • Sarkar D, Pandey SK, Sharma S, 2006. Cytokinins antagoniz the jasmonates action on the regulation of potato (Solanum tuberosum L.) tuber formation in vitro. Plant Cell, Tissue and Organ Culture, 87:285-295.
  • Wazir A, Gul Z, Hussain M, Ullah Khan Z, Saleem M, Khurshid I, 2015. Effect of sucrose on inducing in vitro microtuberization in potato without using any growth hormone. International Journal of Agronomy and Agricultural Research, 7(1): 118-124.
  • Zakaria M, Hossain MM, Khaleque Mian MA, Hossain T, 2014. Performance of different protocols on in vitro tuberization in Potato (Solanum tuberosum). Bangladesh Journal of Agricultural Research, 39(1): 59-66.
  • Zhang ZJ, Zhou WJ, Li HZ, 2005. The role of GA, IAA and BAP in the regulation of in vitro shoot growth and microtuberization in potato (Solanum tuberosum L.). Acta Physiologiae Plantarum, 27: 363- 369.

Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria)

Year 2020, , 723 - 731, 01.06.2020

Samad Khorsandi Alireza Motallebi Azar Fariborz Zaare Nahandi Ali Hatami Sam Mokhtarzadeh

https://doi.org/10.21597/jist.597193
Cited By: 1

Abstract

One of the most critical stages of produced virus free potato plants is rapid and efficient in vitro microtuberization. Plant growth regulators and their concentration is important factor on potato microtuberization. This study was conducted to develop a protocol for rapid microtuber formation for potato micropropagation by different concentrations of 6-Benzylaminopurine (BAP) (0, 1 and 2 mg.l-1) and five concentrations of Putrescine (0, 20, 40, 80 and 160 mg.l-1) with 8% sucrose and 8 g.l-1 agar. Present study was conducted as a factorial experiment based on completely randomized design with three replications in tissue culture laboratory to investigate the effect of different BAP and Putrescine concentrations. Lateral buds of cv. Agria from in vitro shoots were cultured as explants. Cultures were kept in germinator with constant darkness and temperature of 17±2°C. Variance analysis showed that none of the traits were affected by different concentrations of the Putrescine other than weight of the shoots. Also, the interaction effect of PUT×BAP on shoot weight was significant. However, the effect of different levels of BAP was significantly associated with the most trait of microtuberization. In this experiment, the best concentration of BAP for microtuberization was 1 mg.l-1. Also, the appropriate Putrescine level for increasing of microtuberization number and percentage was 80 mg.l-1 but the effect of Putrescine on microtuberization number and percentage was non-significant.

Keywords

Solanum tuberosum in vitro Plant Growth Regulators polyamine

References

  • Akita M, Takayama S, 1994. Induction and development of potato tubers in a jar fermenter. Plant Cell, Tissue and Organ Culture, 36: 177-182.
  • Altindal D, Karadoğan T, 2010. The Effect of Carbon Sources on In Vitro Microtuberization of Potato (Solanum tuberosum L.). Turkish Journal of Field Crops, 15(1): 7-11.
  • Dhital SP, Lim HT, 2012. Microtuberization of Potato (Solanum tuberosum L.) as Influenced by Supplementary Nutrients, Plant Growth Regulators, and In Vitro Culture Conditions, Potato Research, 55 (2): 97-108.
  • Fatima B, Usman M, Ahmad I, Khan IA, 2005. Effect of explant and sucrose on microtuber induction in potato cultivars. International Journal of Agriculture and Biology, 7: 63-66.
  • Gami RA, Parmar SK, Patel PT, Tank CJ, Chauhan RM, Bhadauria HS and Solanki SD, 2013. Microtuberization, minitubers formation and in vitro shoot regeneration from bud sprout of potato (Solanum tuberosum L.) cultivar K. badshah, African Journal of Biotechnology, 12(38): 5640-5647.
  • Garner N, Blake J, 1989. The induction and development of potato microtubers in vitro on media free of growth regulating substances. Annals of Botany, 63:663-674.
  • Gopal J, Minocha JL, Sidhu JS, 1997. Comparative performance of potato crops raised from microtubers induced in dark versus microtubers induced in light. Potato Research, 40: 407-412.
  • Gopal J, Minocha JL, Dhaliwal HS, 1998. Microtuberization in potato (Solanum tuberosum L.). Plant Cell Reports, 16: 794-798.
  • Harmey MA, Crowley MP, Clinch PEM, 1966. The effect of growth regulators on tuberization of cultured stem pieces of Solanum tuberosum. Eur. Potato J, 9: 146-151. Hoque ME, 2010. In Vitro Regeneration Potentiality of Potato under Different Hormonal Combination. World Journal of Agricultural Sciences (WJAS), 6 (6): 660-663.
  • Husain S, Hussain Shah SA, Asghar S, Hussain N, Ali N, Hussain I, Rafiq S, Shah S, Imtiaz M, Ali M, Jala F, Zia-ullah and Rashid M, 2017. Micro-Tuberization of Four Potato (Solanum tuberosum L.) Cultivars Through Tissue Culture. Journal of Botanical Sciences, 6(3): 35-40.
  • Hussey G, Stacey NJ, 1984. Factors affecting the formation of in vitro tubers of potato (Solanum tuberosum L.). Annals of Botany, 53: 565-578.
  • Imai AA, Qhrmanzadeh R, Azimi J, Janpoor J, 2010. The effect of various of 6-benzylaminopurine (BAP) and sucrose on in vitro potato (Solanum tuberosum L.) microtuber induction. American-Eurasian Journal of Agricultural and Environmental Sciences, 8(4): 457-459.
  • Kakkar RK, Nagar PK, Ahuja PS, Rai VK, 2000. Polyamines and plant morphogenesis. Biologia Plantarum, 43: 1-11.
  • Kazemiani S, Motallebi-Azar AR, Panahandeh J, Mokhtarzadeh S, Ozdemir FA, 2018. Shoot proliferation from potato (Solanum tuberosum cv. Agria) under different concentration of MS include vitamins and BAP medium. Progress in Nutrition, 2018; , 20 (1) 160-166.
  • Khuri S, Moorby J, 1996. Nodal segments or microtubers as explants for in vitro microtuber production of potato. Plant Cell, Tissue and Organ Culture, 45: 215-222.
  • Mader JC, 1995. Polyamines in Solanum tuberosum in vitro: Free and conjugated polyamines in hormone-induced tuberization. Journal of Plant Physiology, 146: 115-120.
  • Martin-Tanguy J, 1997. Conjugated polyamines and reproductive development: biochemical, molecular and physiological approaches. Physiologia Plantarum, 100: 675-688.
  • Momena K, Adeeba R, Mehraj H, Jamal Uddin AFM, Saiful Islam and Rahman L, 2014. In Vitro Microtuberization of Potato (Solanum Tuberosum L.) Cultivar through Sucrose and Growth Regulator. Journal of Bioscience and Agriculture Research, 02 (02): 76-82.
  • Murashige T, Skoog F, 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiologia Plantarum, 15: 473−497.
  • Ondo Ovono P, Kevers C, Dommes J, 2009. Tuber formation and development of Dioscorea cayenensis and D. rotundata complex. Effect of Polyamines. In Vitro Cellular & Developmental Biology Plant, 46: 81-88.
  • Pelacho AM, Mingo-Castel AM, 1991. Effects of photoperiod on kinetin-induced tuberization of isolated potato stolons cultured in vitro. American Journal of Potato Research, 86:533-541.
  • Protacio CM, Flores HE, 1992. The role of polyamines in potato tuber formation. In Vitro Cellular & Developmental Biology, 28: 81- 86.
  • Saiprasad GVS, Raghuveer P, 2004. Effect of various polyamines on production of protocorm-like bodies in orchid Dendrobium ‘Sonia’. Scientia Horticulturae, 100: 161-168.
  • Sarkar D, Pandey SK, Sharma S, 2006. Cytokinins antagoniz the jasmonates action on the regulation of potato (Solanum tuberosum L.) tuber formation in vitro. Plant Cell, Tissue and Organ Culture, 87:285-295.
  • Wazir A, Gul Z, Hussain M, Ullah Khan Z, Saleem M, Khurshid I, 2015. Effect of sucrose on inducing in vitro microtuberization in potato without using any growth hormone. International Journal of Agronomy and Agricultural Research, 7(1): 118-124.
  • Zakaria M, Hossain MM, Khaleque Mian MA, Hossain T, 2014. Performance of different protocols on in vitro tuberization in Potato (Solanum tuberosum). Bangladesh Journal of Agricultural Research, 39(1): 59-66.
  • Zhang ZJ, Zhou WJ, Li HZ, 2005. The role of GA, IAA and BAP in the regulation of in vitro shoot growth and microtuberization in potato (Solanum tuberosum L.). Acta Physiologiae Plantarum, 27: 363- 369.
There are 27 citations in total.

Details

Primary Language English
Subjects Horticultural Production
Journal Section Bahçe Bitkileri / Horticulture
Authors

Samad Khorsandi This is me 0000-0002-1438-0433

Alireza Motallebi Azar This is me 0000-0002-4384-3716

Fariborz Zaare Nahandi This is me 0000-0003-3253-8705

Ali Hatami This is me 0000-0003-2989-6296

Sam Mokhtarzadeh 0000-0002-3927-0855

Publication Date June 1, 2020
Submission Date July 26, 2019
Acceptance Date March 1, 2020
Published in Issue Year 2020

Cite

APA Khorsandi, S., Motallebi Azar, A., Zaare Nahandi, F., Hatami, A., et al. (2020). Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria). Journal of the Institute of Science and Technology, 10(2), 723-731. https://doi.org/10.21597/jist.597193
AMA Khorsandi S, Motallebi Azar A, Zaare Nahandi F, Hatami A, Mokhtarzadeh S. Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria). Iğdır Üniv. Fen Bil Enst. Der. June 2020;10(2):723-731. doi:10.21597/jist.597193
Chicago Khorsandi, Samad, Alireza Motallebi Azar, Fariborz Zaare Nahandi, Ali Hatami, and Sam Mokhtarzadeh. “Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria)”. Journal of the Institute of Science and Technology 10, no. 2 (June 2020): 723-31. https://doi.org/10.21597/jist.597193.
EndNote Khorsandi S, Motallebi Azar A, Zaare Nahandi F, Hatami A, Mokhtarzadeh S (June 1, 2020) Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria). Journal of the Institute of Science and Technology 10 2 723–731.
IEEE S. Khorsandi, A. Motallebi Azar, F. Zaare Nahandi, A. Hatami, and S. Mokhtarzadeh, “Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria)”, Iğdır Üniv. Fen Bil Enst. Der., vol. 10, no. 2, pp. 723–731, 2020, doi: 10.21597/jist.597193.
ISNAD Khorsandi, Samad et al. “Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria)”. Journal of the Institute of Science and Technology 10/2 (June 2020), 723-731. https://doi.org/10.21597/jist.597193.
JAMA Khorsandi S, Motallebi Azar A, Zaare Nahandi F, Hatami A, Mokhtarzadeh S. Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria). Iğdır Üniv. Fen Bil Enst. Der. 2020;10:723–731.
MLA Khorsandi, Samad et al. “Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria)”. Journal of the Institute of Science and Technology, vol. 10, no. 2, 2020, pp. 723-31, doi:10.21597/jist.597193.
Vancouver Khorsandi S, Motallebi Azar A, Zaare Nahandi F, Hatami A, Mokhtarzadeh S. Effect of Different Concentrations of BAP and Putrescine on Potato Microtuberization (cv. Agria). Iğdır Üniv. Fen Bil Enst. Der. 2020;10(2):723-31.

Cited By

Microtuberization Potential of Jasmonic Acid, Kinetin and Putrescine in Potato (Solanum tuberosum L.)
American Journal of Potato Research
https://doi.org/10.1007/s12230-023-09905-7