Biochemical Investigation of Effects of Copolymers on Corn Plants Under Salt Stress

Abstract

In this study, in order to investigate possible applications in agriculture of biodegradable poly (ethylene oxide) -co- (ε-caprolactone) [poly (EO-co-ε-CL)] copolymer synthesized in 4 different ratios, growth analysis was performed in corn plants. Copolymers, 200 mM salt (NaCl) solution and both copolymers and salt solution were applied to the corn together and compared with the control groups. To evaluate the effect of copolymers and salinity on plant growth, some biochemical analyzes such as lipid peroxidation, pigment and total carbohydrate content were performed. It was determined that copolymers had a positive effect on malondialdehyde (MDA) a product of lipid peroxidation and total carbohydrate content as well as chlorophyll content. Furthermore, poly (EO-co-ε-CL1:4) of these copolymers was more effective than other copolymers in preventing the harmful effects of salinity on the growth of corn plants. As a result, it was observed that all parameters examined were negatively affected by salt stress, while copolymer application had a positive effect.

Keywords

• Biodegradable copolymer,salt stress,corn,MDA,chlorophyll

Project Number

(MFMUB017-08)

Kopolimerlerin Tuz Stresi Altındaki Mısır Bitkilerine Etkisinin Biyokimyasal Olarak İncelenmesi

Abstract

Bu araştırmada, biyobozunur özellik gösteren ve 4 farklı oranda sentezlenen poli(etilen oksit)-ko-(ε-kaprolakton) [poli(EO-ko-ε-CL)] kopolimerinin tarımda tuz stresine karşı cevabını incelemek için mısır (Zea mays L. cv. “72 May 99”) bitkilerine uygulama yapılmıştır. Mısıra, kopolimerler, 200 mM tuz (NaCl) çözeltisi ve hem kopolimerler hem de tuz çözeltisi birlikte uygulanarak kontrol gruplarıyla karşılaştırılmıştır. Kopolimerlerin ve tuzluluğun bitki gelişimine etkisini değerlendirmek için lipid peroksidasyonu, pigment ve toplam karbonhidrat içeriği gibi bazı biyokimyasal analizler yapılmıştır. Kopolimerlerin, klorofil içeriğinin yanı sıra lipid peroksidasyon içeriğinin bir ürünü olan malondialdehit (MDA) ve toplam karbonhidrat içeriği üzerinde de olumlu bir etkisi olduğu saptanmıştır. Ayrıca mısır bitkisinde bu kopolimerlerden poli (EO-ko-ε-CL1:4)’ün, tuzluluğun zararlı etkilerinin önlenmesinde diğer kopolimerlerden daha etkili olduğu gözlenmiştir. Sonuç olarak, incelenen tüm parametrelerin tuz stresinden olumsuz etkilendiği görülürken, kopolimer uygulamasının tuz stresine cevapta olumlu etki oluşturduğu saptanmıştır.

Keywords

• Biyobozunur kopolimer,tuz stresi,mısır,MDA,klorofil

Supporting Institution

Munzur Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

(MFMUB017-08)

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