Farmasötik Formülasyonlardaki İkili İlaç Bileşenlerinin Eş Zamanlı Olarak Kemometrik Metotlarla Tayini

Abstract

Farmasötik formülasyonlardaki parasetamol ve amoksisilin miktarlarının spektrofotometrik olarak eş zamanlı tayini yapılmıştır. Çok değişkenli kalibrasyon tekniklerinden temel bileşen regresyonu (TBR) ve kısmi en küçük kareler yöntemi (KEKK) kullanılmıştır. Spektrofotometrik-kemometrik metotlar kullanılarak, hem laboratuvarda hazırlanan sentetik karışımların hem de ilaç tabletlerinin hiçbir ön ayırma yapılmadan analizi sağlanmıştır. Sentetik karışımlardaki her aktif madde için kalibrasyon eğrilerinin doğrusallığı, konsantrasyon aralıklarının uygun olduğu gözlenmiştir (r2> 0,9997). İki yöntemin doğruluğu ve tekrarlanabilirliği incelendiğinde yüksek geri kazanım ve düşük standart sapma değerleri elde edilmiştir. Yüksek geri kazanım ve düşük standart sapma değerleri, bu çalışmada ilaç tablet analizleri konusunda bizi cesaretlendirmiştir. Önerilen metotlar, farmasötik örneklerdeki aktif bileşenlerin tayininin başarıyla uygulanması yönünden oldukça yüksek kesinlik ve duyarlılığa sahiptir.

Keywords

• Parasetamol,Amoksisilin,PLS,PCR

Simultaneous Determination of Binary Drug Components in Pharmaceutical Formulations with Chemometric Methods

Abstract

A synchronous definition of paracetamol and amoxicillin quantities in pharmaceutical formulations was performed using spectrophotometric methods. Principal component regression (PCR) and partial least squares regression (PLS) were used as multivariate calibration techniques. Spectrophotometric-chemometric methods were used for the simultaneous quantification of paracetamol and amoxicillin in the laboratory prepared mixtures and in the drug tablets without any requirements for a prior separation. The linearity of the calibration curves for each active substance in the synthetic mixtures demonstrated that concentration ranges were appropriate (r2>0.9997). The investigation of the accuracy and repeatability of the two methods resulted in high recovery rates and lower standard deviation values. Achieving high recovery and low standard deviation values, this study encouraged us to proceed further in pharmaceutical assay. The recommended methods are highly sensitive and precise as these methods have been successfully applied to quantify active substances in pharmaceutical samples.

Keywords

• Paracetamol,Amoxicillin,PLS,PCR

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