The Protective Effect of Zingeron Against Salt Stress in Barley

Abstract

Among the abiotic stresses, drought is the most restrictive factor in the production of agricultural products. And the salinity comes after drought as a second major factor. It is foreseen that this problem will get worse as an increase in the salinity of the soil is predicted due to climatic changes. Environmental stress factors cause a decrease in the defense system activities of organisms and an increase in the reactive oxygen species (ROS). The increasing ROSs cause DNA and RNA damage. In this study, it is aimed to determine the protective effect of zingeron in Hordeum vulgare L. seeds exposed to salt stress in these 5 different doses (50, 100, 150, 200 ve 250 mM). IRAP (Inter Retrotransposon Amplified Polymorphism) technique has been used to determine the values of GTS (Genomic Template Stability) and polymorphisms constituted of the retrotransposon mobility triggered by the salinity stress. According to the results of IRAP analysis, polymorphism values of barley samples exposed to salt stress vary between 40.74% and 18.51% and the average is 28.88%. GTS value varies between 81.49% and 59.26% and its average is 71.12%. In addition to salt stress, the value of polymorphism decreased in the range of 14.81% to 33.33% in barley samples where zingeron was applied, and the average decreased to 24.43%. GTS value increased from 66.67% to 85.19%, and its average increased to 75.57%. It was determined from the study results that zingeron alleviates salt stress and reduces retrotansposon mobility.

Keywords

• Hordeum vulgare l.
• IRAP
• LTR-Retrotanspozon
• salt stress
• zingerone

Arpada Tuz Stresine Karşı Zingeronun Koruyucu Etkisi

Abstract

Abiyotik stresler içerisinde tarım ürünlerinin üretimini en çok sınırlayan kuraklık olup, bunu tuzluluk faktörü takip etmektedir. İklimsel değişikler nedeniyle toprağın tuzluluk oranında artış beklendiği için bu sorunun daha da kötüleşeceği öngörülmektedir. Çevresel stres faktörleri, organizmaların savunma sistem aktivitelerinin azalmasına ve reaktif oksijen türlerinin (ROT) artmasına neden olmaktadır. Artan ROT’ lar ise DNA ve RNA hasarına neden olurlar. Bu çalışmada 5 farklı dozda (50, 100, 150, 200 ve 250 mM) tuz stresine maruz bırakılan Hordeum vulgare L. tohumlarında zingeronun koruyucu etkisinin tespiti amaçlanmıştır. Tuz stresinin tetiklediği retrotranspozonların hareketliliği ile oluşan polimorfizm ve GTS (Genomic Template Stability) seviyelerinin tespiti için IRAP (Inter Retrotransposon Amplified Polymorphism) tekniği kullanılmıştır. IRAP analizi sonuçlarından, tuz stresine maruz kalan arpa örneklerinin polimorfizm değerleri %18.51 ile %40.74 arasında değişiklik göstermekte olup ortalaması % 28.88’ dir. GTS değeri ise % 59.26 ile % 81.49 arasında değişiklik göstermekte ve ortalaması % 71.12’ dir. Tuz stresinin yanı sıra zingeron uygulanan arpa örneklerinde ise polimorfizm değeri azalarak % 14.81 ile % 33.33 aralığında elde edilmiş ve ortalaması ise % 24.43’e düşmüştür. GTS değeri % 66.67 ile % 85. 19 aralığına yükselmiş, ortalaması ise % 75.57’ ye çıkmıştır. Çalışma sonuçlarından zingeronun tuz stresini hafiflettiği ve retrotanspozon hareketliliğini azalttığı saptanmıştır.

Keywords

• Hordeum vulgare
• ırap
• ltr-retrotranspozon
• tuz stresi
• zingeron

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