Farklı Dozlarda Ekzojen Gonadotropin Uygulamasının Serotonin ve Melatonin Düzeylerine Etkisi

Abstract

Ekzojen gonadotropinler vasıtasıyla ovaryum stimülasyonu (OS) hem üreme biyolojisi ile ilgili deneysel çalışmalarda hem de in vitro fertilizasyon (IVF) ’da yaygın olarak kullanılan bir uygulamadır. Fakat yapılan bazı çalışmalardan elde edilen veriler OS’nin oosit, embriyo niteliği ve embriyogenez süreçlerine çeşitli olumsuz etkilerinin olabileceğine işaret etmektedir. Dolayısıyla, OS prosedürlerinin üreme ve diğer biyolojik süreçlerle olan olası etkilerini inceleyecek kapsamlı çalışmalara ihtiyaç duyulduğu düşünülmektedir. Serotonin ve melatoninin de oosit ve embriyo niteliğinde etkileri olan önemli hormonlar olduğu belirtilmektedir. Bununla birlikte farklı dozlarda uygulanan ekzojen gonadotropin uygulamalarının bu hormonlar üzerine etkisini inceleyen herhangi bir araştırma olmadığı anlaşılmaktadır. Bu nedenle bu araştırmada farklı dozlarda ekzojen gonadotropin uygulamasının serotonin ve melatonin düzeylerine etkisini fare modelinde incelemeyi amaçladık. Çalışmamızda fareler 5, 7.5, 10 ve 12.5 I.U. Gebe Kısrak Serum Gonadotropin/ İnsan Koriyonik Gonadotropin (PMSG/hCG) uygulamak üzere 4 farklı gruba (n=6) ayrıldı. Ekzojen gonadotropinlerle muamele sonrasında melatonin ve serotoninin düzeyleri çalışma gruplarına ait plazma örneklerinde ELISA yöntemi ile tespit edildi. Melatonin düzeyleri yönünden gruplar arasında anlamlı bir fark belirlenmezken, serotonin düzeyleri yönünden gruplar arasında anlamlı bir farkın olduğu gözlenmiştir. Yapılan grup içi karşılaştırmalarda 7.5 I.U. PMSG/hCG uygulanan grupta diğer gruplara nazaran serotonin düzeyleri yönünden anlamlı bir artışın olduğu saptandı. Sonuç olarak uyguladığımız ekzojen gonadotropin dozlarının melatonin düzeylerini etkilemediği ancak serotonin seviyelerini 7.5 I.U. PMSG/hCG uygulaması sonrasında diğer dozlara göre anlamlı olarak artırdığı anlaşılmaktadır. Daha yüksek ekzojen gonadotropin dozlarının bu hormonlara etkisini hayvanlarda ve insanlarda da inceleyecek ilave çalışmaların üreme biyolojisine katkı sunacağı düşünülmektedir.

Keywords

• Serotonin
• melatonin
• gonadotropin
• ovaryum stimülasyonu

Effect of Different Doses of Exogenous Gonadotropin Administration on Serotonin and Melatonin Levels

Abstract

Ovarian stimulation (OS) by exogenous gonadotropins is a common technique both in experimental studies on reproductive biology and in vitro fertilization (IVF). However, the data obtained from some studies indicate that OS may have various negative effects on the quality of oocyte and embryo, and embryogenesis processes. Therefore, it is thought that further studies are needed to examine the possible effects of OS procedures on reproductive and other biological processes. It is reported that serotonin and melatonin are important hormones that have effects on the quality of oocyte and embryo. On the other hand, it is understood that there is no study to examine the effect of different doses of exogenous gonadotropin treatment on these hormones. Therefore, in this study, we aimed to examine the effects of different doses of exogenous gonadotropin on serotonin and melatonin levels in the mouse model.In our study, mice were divided into 4 different groups (n = 6) to treatment with 5, 7.5, 10 and 12.5 I.U. Pregnant Mare Serum Gonadotrophin/ Human Chorionic Gonadotropin ( PMSG/hCG). After the treatment with exogenous gonadotropins, levels of melatonin and serotonin were determined by the ELISA method in the plasma samples of the study groups. While there was no significant difference between the groups in terms of melatonin levels, there was a significant difference between the groups in terms of serotonin levels. In intragroup comparisons, it was found that there was a significant increase in the serotonin levels in the group treated with 7.5 IU PMSG/hCG compared to other groups.In conclusion, it is understood that the exogenous gonadotropin doses, which we administrated, did not affect melatonin levels but significantly increased serotonin levels after 7.5 I.U. PMSG/hCG administration compared to other doses. Additional studies to examine the effect of higher exogenous gonadotropin doses on these hormones in animals and also in humans, are thought to contribute to reproductive biology.

Keywords

• Serotonin
• melatonin
• gonadotropin
• ovarian stimulation

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