Expression Patterns of Oxidative Stress-Related Genes of Cucurbita pepo and Relation to Cellular H2O2 under Short-Term Heavy Metal Stress

Abstract

Oxidative stress caused by biotic and abiotic stress factors is the most important cause of cellular damage. Due to their sessile structures, plants have evolved regulatory mechanisms to respond to various environmental stresses The increased cellular concentration of reactive oxygen species is one of the major consequences of oxidative stress. including H2O2 production. Also, H2O2 is produced as a by-product of respiratory and photosynthetic metabolisms in plants. H2O2 acts as a multifaceted molecule because of its dual role in cells. It has been found to act as a secondary messenger in signal transmission networks. In this study, the changes in expression levels of stress-related genes and their relationship with H2O2 in pumpkin (Cucurbita pepo) plant exposed to Cd heavy metal at different durations and concentrations were investigated. As a result of this study, we concluded that the expression of stress-related genes may be related to the oxidative status of the cell and the concentration of H2O2 in the signaling mechanism, the expression of stress-related genes may be up-regulated to a certain degree of concentration, while a higher concentration of H2O2 may down-regulate the expression of the genes.

Keywords

• Stress-related genes
• expression level
• Oxidative stress
• Cadmium
• Reactive Oxigen Species
• Cucurbita pepo

Project Number

NEUBAP YLTPF24

References

1. Ahmad P, 2014. Oxidative Damage to Plants: Antioxidant Networks and Signaling. Oxidative Damage to Plants: Antioxidant Networks and Signaling.
2. Apel K, Hirt H, 2004. Reactıve Oxygen Specıes: Metabolism, Oxidative Stress, and Signal Transduction. Annual Review of Plant Biology (55):373–399.
3. Azarabadi S, Abdollahi H, Torabi M, Salehi Z, Nasiri J, 2017. ROS generation, oxidative burst and dynamic expression profiles of ROS-scavenging enzymes of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) in response to Erwinia amylovora in pear (Pyrus communis L). European Journal of Plant Pathology (147):279–294.
4. Baxter A, Mittler R, Suzuki N, 2014. ROS as key players in plant stress signalling. Journal of Experimental Botany (65):1229–1240.
5. Boyer JS, 1982. Plant productivity and environment. Science (New York, N.Y.) (218):443–448.
6. Brunet J, Varrault G, Zuily-Fodil Y, Repellin A, 2009. Accumulation of lead in the roots of grass pea (Lathyrus sativus L.) plants triggers systemic variation in gene expression in the shoots. Chemosphere (77): 1113-1120.
7. Cantarello C, Volpe V, Azzolin C, Bertea CM, 2005. Modulation of enzyme activities and expression of genes related to primary and secondary metabolism in response to UV-B stress in cucumber (Cucumis sativus L.). Journal of Plant Interactions.
8. Choudhury FK, Rivero RM, Blumwald E, Mittler R, 2017. Reactive oxygen species, abiotic stress and stress combination. Plant Journal (90):856–867.

9. Cuypers A, Hendrix S, Amaral dos Reis R, De Smet S, Deckers J, Gielen H, Jozefczak M, Loix C, Vercampt H, Vangronsveld J, Keunen E, 2016. Hydrogen Peroxide, Signaling in Disguise during Metal Phytotoxicity. Frontiers in Plant Science (7):.
10. Dai Q lin, Chen C, Feng B, Liu T ting, Tian X, Gong Y ya, Sun Y kun, Wang J, Du S zhang, 2009. Effects of different NaCL concentration on the antioxidant enzymes in oiLseed rape (Brassica napus L.) seedlings. Plant Growth Regulation.
11. Del Río LA, 2015. ROS and RNS in plant physiology: An overview. Journal of Experimental Botany (66):2827–2837.
12. Dempsey DA, Klessig DF, 1994. Salicylic acid, active oxygen species and systemic acquired resistance in plants. Trends in Cell Biology.
13. Dixit V, Pandey V, Shyam R, 2001. Differential antioxidative responses to cadmium in roots and leaves of pea (Pisum sativum L. cv. Azad). Journal of Experimental Botany.
14. Farnese FS, Menezes-Silva PE, Gusman GS, Oliveira JA, 2016. When Bad Guys Become Good Ones: The Key Role of Reactive Oxygen Species and Nitric Oxide in the Plant Responses to Abiotic Stress. Frontiers in plant science (7):471.
15. Gill SS, Tuteja N, 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry (48):909–930.
16. Gupta K, Sengupta A, Chakraborty M, Gupta B, 2016. Hydrogen Peroxide and Polyamines Act as Double Edged Swords in Plant Abiotic Stress Responses. Frontiers in plant science (7):1343.
17. Hassinen VH, Tervahauta AI, Schat H, Kärenlampi SO, 2011. Plant metallothioneins - metal chelators with ROS scavenging activity? Plant Biology.
18. Heath RL, Packer L, 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics (125):189–198.
19. Hossain MA, Bhattacharjee S, Armin SM, Qian P, Xin W, Li HY, Burritt DJ, Fujita M, Tran LSP, 2015. Hydrogen peroxide priming modulates abiotic oxidative stress tolerance: insights from ROS detoxification and scavenging. Frontiers in Plant Science (6):1–19.
20. Jain R, Verma R, Singh A, Chandra A, Solomon S, 2015. Influence of selenium on metallothionein gene expression and physiological characteristics of sugarcane plants. Plant Growth Regulation.
21. Junglee S, Urban L, Sallanon H, Lopez-lauri F, 2014. Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide. American Journal of Analytical Chemistry (5):730–736.
22. Kar M, 2018. Determination of the expression level of stress-related genes in Cicer arietinum root cell under Cd stress and the relationship to H2O2 concentrations. Ecotoxicology (27):1087–1094.
23. Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen G, Mullineaux P, 1999. Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis. Science.
24. Khan MN, Mobin M, Abbas ZK, AlMutairi KA, Siddiqui ZH, 2017. Role of nanomaterials in plants under challenging environments. Plant Physiology and Biochemistry (110):194–209.
25. Laloi C, Apel K, Danon A, 2004. Reactive oxygen signalling: The latest news. Current Opinion in Plant Biology.
26. Liochev SI, Fridovich I, 2007. The effects of superoxide dismutase on H2O2 formation. Free Radical Biology and Medicine.
27. Luna CM, Pastori GM, Driscoll S, Groten K, Bernard S, Foyer CH, 2005. Drought controls on H2O2 accumulation, catalase (CAT) activity and CAT gene expression in wheat. Journal of Experimental Botany (56):417–423.
28. Luo H, Li H, Zhang X, Fu J, 2011. Antioxidant responses and gene expression in perennial ryegrass (Lolium perenne L.) under cadmium stress. Ecotoxicology (20):770–778.
29. Rossatto T, do Amaral MN, Benitez LC, Vighi IL, Braga EJB, de Magalhaes Junior AM, Maia MAC, da Silva Pinto L, 2017. Gene expression and activity of antioxidant enzymes in rice plants, cv. BRS AG, under saline stress. Physiology and molecular biology of plants : an international journal of functional plant biology (23):865–875.
30. Rout JR, Sahoo SL, 2013. Antioxidant enzyme gene expression in response to copper stress in Withania somnifera L. Plant Growth Regulation (71):95–99.
31. Souguir D, El Ferjani E, Ledoigt G, Goupil P, 2013. Transcript accumulation of stress-related genes in Vicia faba roots under a short exposure to cadmium. Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology (3504):1–9.
32. Suzuki N, Miller G, Salazar C, Mondal HA, Shulaev E, Cortes DF, Shuman JL, Luo X, Shah J, Schlauch K, Shulaev V, Mittler R, 2013. Temporal-Spatial Interaction between Reactive Oxygen Species and Abscisic Acid Regulates Rapid Systemic Acclimation in Plants. The Plant Cell.
33. Tamás L, Dudíková J, Ďurčeková K, Halušková L, Huttová J, Mistrík I, Ollé M, 2008. Alterations of the gene expression, lipid peroxidation, proline and thiol content along the barley root exposed to cadmium. Journal of Plant Physiology (165):1193–1203.
34. Tombuloglu H, Semizoglu N, Sakcali S, Kekec G, 2012. Boron induced expression of some stress-related genes in tomato. Chemosphere (86):433–438.
35. Tony R, Suzy VS, Kelly O, Els K, Frank VB, Cuypers A, Jos R, Karen S, Jan C, Jaco V, Nathalie V, Yves G, Nele H, 2010. The cellular redox state as a modulator in cadmium and copper responses in Arabidopsis thaliana seedlings. Journal of Plant Physiology (168):309–316.
36. Vanderauwera S, Hoeberichts FA, Breusegem F Van, 2009. Hydrogen Peroxide-Responsive Genes in Stress Acclimation and Cell Death. Reactive Oxygen Species in Plant Signaling: Springer., pp. 149–164.
37. Wang L, Yang L, Yang F, Li X, Song Y, Wang X, Hu X, 2010. Involvements of H2O2 and metallothionein in NO-mediated tomato tolerance to copper toxicity. Journal of Plant Physiology (167):1298–1306.
38. Zimmermann P, Heinlein C, Orendi G, Zentgraf U, 2006. Senescence-specific regulation of catalases in Arabidopsis thaliana (L.) Heynh. Plant, Cell and Environment (29):1049–1060.