Tuz Stresine Maruz Bırakılan Kanola (Brassica napus L.)’da Priming Uygulamalarının (Salisilik Asit ve Askorbik Asit) Çimlenme Üzerine Etkisi

Abstract

Bu araştırmada, tuz stresine maruz bırakılan kanola (Brassica napus L.) tohumlarına salisilik asit (SA) ve askorbik asit (AsA) ile priming yapılarak çimlenmeye etkilerinin belirlenmesi ve bu uygulamaların optimum konsantrasyonlarının tespit edilmesi amaçlanmıştır. Araştırmada, 5 farklı NaCI konsantrasyonu (kontrol, 50, 100, 150, 200 mM) ile 2 farklı priming uygulaması (SA ve AsA ) ve her priming uygulamasının 5 farklı konsantrasyonu (kontrol, 0.5, 1.0, 1.5, 2.0 mM) ele alınmıştır. Çalışma tesadüf parsellerinde faktöriyel deneme desenine göre dört tekerrürlü olarak yürütülmüştür. Araştırma sonucunda çimlenme oranı (%), ortalama çimlenme süresi (gün) ve çimlenme indeksi (hızı) değerleri incelenmiştir. SA uygulamaları arasında, 0.5 mM SA konsantrasyonunun, artan tuz dozlarında tuz stresinin olumsuz etkisini azaltarak çimlenmeyi hızlandırdığı, ortalama çimlenme süresini kısalttığı ve çimlenme oranını artırdığı tespit edilmiştir. SA konsantrasyonu artıkça (1.5 mM ve 2.0 mM SA) tüm tuz uygulamalarında çimlenmenin olumsuz etkilendiği belirlenmiştir. AsA uygulamaları arasında ise 0.5, 1.0, 1.5 mM konsantrasyonlarının, tuz stresinin olumsuz etkisini büyük ölçüde azaltarak çimlenme oranı ve çimlenme indeksi değerlerine olumlu etkide bulunduğu belirlenirken, ortalama çimlenme süresi üzerine etki etmediği tespit edilmiştir. Kanola tonumlarına çimlenme öncesi uygulanan SA ve AsA ile priming uygulamalarının, tuz stresinin çimlenme engelleyici etkisini önemli ölçüde ortadan kaldırdığı saptanmış olup, 0.5 mM SA/AsA konsantrasyonları optimum doz olarak belirlenmiştir.

Keywords

• Kanola
• Çimlenme
• Tuz
• Priming
• Salisilik asit
• Askorbik asit

Effect of Priming Applications (Salicylic Acid and Ascorbic Acid) on Germination in Canola Under Salt Stress (Brassica napus L.)

Abstract

In this study, it was aimed to determine the effects of germination of canola (Brassica napus L.) seeds on germination by priming with salicylic acid (SA) and ascorbic acid (AsA) and to determine the optimum concentrations of these applications. In the study, 5 different NaCI concentrations (control, 50, 100, 150, 200 mM) and 2 different priming applications (SA and AsA) and 5 different concentrations (control, 0.5, 1.0, 1.5, 2.0 mM) of each priming application were discussed. The experiment was established in randomized plots with 4 replications according to the factorial design. As a result of the research, the germination rate (%), mean germination time (days) and germination index were examined. Among SA applications, 0.5 mM SA concentration was found to reduce the negative effect of salt stress at increasing salt doses, accelerate germination, shorten the average germination time and increase the germination rate. As the SA concentration increased (1.5 mM and 2.0 mM SA) it was determined that germination was negatively affected in all salt applications. Among AsA applications, it was determined that 0.5, 1.0, 1.5 mM concentrations had a positive effect on the germination rate and germination index values by decreasing the negative effect of salt stress, while not affecting the average germination time. Priming applications with SA and AsA, which we applied to canola seeds before germination, have been found to significantly eliminate the germination inhibitory effect of salt stress, and 0.5 mM SA / AsA concentrations were determined optimally.

Keywords

• Priming
• Canola
• Germination
• Salt
• Salicylic acid
• Ascorbic acid

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