Creation and Characterization of Edible Coating Films: Application on Orange and Lemon Fruits

Abstract

In this study, the variation of the shelf life of lemon and orange fruits with edible coatings was investigated. In the study, four different coatings were created with three different type ingredients as carnauba wax-based, carnauba wax-shellac-based and shellac-based and applied to fruits. While the preparation of coatings, the magnetic stirring speed was kept at 400 rpm and the temperature were kept at 85 ° C. The water solubility and moisture content of created formulations were investigated. Antimicrobial activities of the coatings were investigated by disk diffusion method. The coatings prepared within the scope of the study were coated on the fruits and physical and chemical analyzes were applied. Fruits were observed for 12 days at +4 ° C refrigerator and room conditions. It has been determined that the solubility of the coatings formed by Formula 1, 2 and 3 in water is lower than the coatings formed by Formula 4. It has been determined that coatings formed with formulas containing carnauba wax have lower water solubility than dry films formed with formulas containing shellac. The best results were obtained with Formula 1 in coated fruits, observed at both refrigerator and room temperature. The coatings formed have been found to have antimicrobial activity against both gram positive and gram negative bacterias.

Keywords

• Fruit coating
• weight loss
• shellac
• carnauba wax
• citrus

Project Number

PY17007

Yenilebilir Kaplama Filmlerinin Oluşturulması ve Karakterizasyonu: Portakal ve Limon Meyvelerinde Uygulanması

Abstract

Bu araştırmada limon ve portakal meyvelerinin yenilebilir kaplamalar ile raf ömürlerinin değişimi incelenmiştir. Çalışmada karnauba vaks bazlı, karnauba vaks-şellak bazlı ve şellak bazlı üç farklı türde dört farklı kaplama formüle edilerek meyvelere uygulanmıştır. Kaplamalar oluşturulurken manyetik karıştırma hızı 400 rpm’de ve sıcaklık 85°C’de tutulmuştur. Kaplamaların sudaki çözünürlükleri, nem içerikleri ve disk difüzyon yöntemi ile antimikrobiyal aktiviteleri incelenmiştir. Çalışma kapsamında hazırlanan kaplamalar meyvelere kaplanarak fiziksel ve kimyasal analizler uygulanmıştır. Meyveler 12 gün boyunca +4 °C buzdolabında ve oda koşullarında gözlemlenmiştir. Kaplamaların nem içerikleri, sudaki çözünürlükleri, anti-mikrobiyal aktiviteleri ve kütle kaybı analizleri yapılmıştır. Formül 1,2 ve 3 ile oluşturulan kaplamaların sudaki çözünürlüklerinin, Formül 4 ile oluşturulan kaplamalara göre daha düşük olduğu belirlenmiştir. Hem buzdolabı hem de oda sıcaklığında gözlemlenen, kaplama yapılmış, meyvelerde en iyi sonuçlar Formül 1 ile elde edilmiştir. Oluşturulan kaplamalar hem gram pozitif hem de gram negatif bakterilere karşı antimikrobiyal aktiviteye sahip olduğu tespit edilmiştir.

Keywords

• Meyve kaplama
• kütle kaybı
• şellak
• karnauba vaks
• narenciye

Supporting Institution

Petro Yağ ve Kimyasallar San. ve Tic. A.Ş

Project Number

PY17007

References

1. Akbudak B, Eriş A, Tezcan H, Karabulut ÖA, 2002. Kiraz muhafazasında farklı uygulamaların kalite ve fungal hastalıklar üzerine etkisi. II. Bahçe Ürünlerinde Muhafaza ve Pazarlama Sempozyumu, 24-27 Eylül 2002, Çanakkale.
2. Akbulut M, Özcan M, 1997. Kirazlarda farklı ambalaj tiplerinin muhafaza süre ve kaliteleri üzerine etkileri. Bahçe Ürünlerinde Muhafaza ve Pazarlama Sempozyumu, 21-24 Ekim 1997, Yalova.
3. Álvaro ML, Miguel AC, Bartolomeu WSS, Ed CM, José AT, Renato AM, António AV, 2010. New edible coatings composed of galactomannans and collagen blends to improve the postharvest quality of fruits – Influence on fruits gas transfer rate. Journal of Food Engineering, 97 (1): 101–109.
4. Azeredo HM, Miranda KW, Ribeiro HL, Rosa MF, Nascimento DM, 2012. Nanoreinforced alginate–acerola puree coatings on acerola fruits. Journal of Food Engineering, 113: 505–510.
5. Bai J, Hagenmaier RD, Baldwin EA, 2003. Coating selection for ‘Delicious’ and other apples. Postharvest Biology and Technology, 28 (3): 381–390.
6. Certel M, Uslu MK, Özdemir F, 2004. Effects of sodium caseinate and milk protein concentrate-based edible coatings on the postharvest quality of bing cherries. Journal of the Science of Food and Agricultural, 84 (10): 1229-1234.
7. Cemeroğlu, B.S. 2011. Meyve Sebze İşleme Teknolojisi. Nobel Yayınevi 1.cilt s.250-315 Ankara-Türkiye.
8. Chen S, Nussinovitch A, 2000. Galactomannans in disturbances of structured wax–hydrocolloid-based coatings of citrus fruit (easy-peelers). Food Hydrocolloids, 14 (6): 561–568.

9. Chiumarelli M, Hubinger MD, 2014. Evaluation of edible films and coatings formulated with cassava starch, glycerol, carnauba wax and stearic acid. Food Hydrocolloids, 38: 20–27.
10. Hızal T, Karlı B, 2020. Örtüaltı sebze üretiminde üreticilerin tohum tercihini etkileyen faktörlerin belirlenmesi: Antalya ili örneği. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 5 (3): 383–93.
11. Kocak H, Bal E, 2016. Hasat Sonrası UV-C ve Yenilebilir Yüzey Kaplama Uygulamalarının Kiraz Meyve Kalitesi ile Muhafaza Süresi Üzerine Etkileri. Türkiye Tarımsal Araştırmalar Dergisi, 4 (1): 79-88.
12. Lee HB, Noh BS, Min SC, 2012. Listeria monocytogenes inhibition by defatted mustard meal- based edible films. International Journal of Food Microbiology, 153: 99 –105.
13. Marmur T, Elkind Y, Nussinovitch A, 2013. Increase in gloss of coated red peppers by different brushing procedures. LWT- Food Science and Technology, 51 (2): 531–536.
14. Mehyar GF, Al-Qadırı HM, Abu-Blan HA, Swanson BG, 2011. Antifungal effectiveness of potassium sorbate incorporated in edible coatings against spoilage molds of apples, cucumbers, and tomatoes during refrigerated storage. Journal of Food Science, 76: M210–7.
15. Nussinovitch A, 2009. CHAPTER 10 - Biopolymer Films and Composite Coatings. Academic Press, pp.295–326 Cambridge-Massachusetts.
16. Pe´rez-Gago MB, Krochta JM, 2005. Emulsion and bi-layer edible films. In: Han, J.H. (Ed.), Innovations in Food Packaging. Elsevier, pp.362383 London-England.
17. Reuther W, Calavan EC, 1989. The citrus Industry. University of California Division of Agricultural and Natural Resources United States-America.
18. Sabır FK, Ağar İT, 2008. Farklı özelliklere sahip modifiye atmosfer poşetlerde muhafazasının 0900 ziraat çeşidinde muhafaza süresi ve kalite üzerine etkileri. Bahçe Ürünlerinde IV. Muhafaza ve Pazarlama Sempozyumu, 8-11 Ekim 2008, Antalya-Türkiye.
19. Saki N, Akin M, Atsay A, Karaoglu HRP, Kocak MB, 2018. Synthesis and Characterization of Novel Quaternized 2,3-(Diethylmethylamino)Phenoxy Tetrasubstituted Indium and Gallium Phthalocyanines and Comparison of Their Antimicrobial and Antioxidant Properties with Different Phthalocyanines. Inorganic Chemistry Communications, 95: 122–129.
20. Valencıa-Chamorro SA, Perez-Gago MB, Del Rıo MA, Palou L, 2009. Curative and preventive activity of hydroxypropyl methylcellulose- lipid edible composite coatings containing antifungal food additives to control citrus postharvest green and blue molds. Journal of Agricultural and Food Chemistry, 57: 2770–7.
21. Valencıa-Chamorro SA, Perez-Gago MB, Del Rıo MA, Palou L, 2010. Effect of antifungal hydroxypropyl methylcellulose- lipid edible composite coatings on Penicillium decay development and postharvest quality of cold- stored ‘Ortanique’ mandarins. Journal of Food Science, 75: S418–26.
22. Velickova E, Winkelhausen E, Kuzmanova S, Alves VD, Moldão-Martins M, 2013. Impact of chitosan-beeswax edible coatings on the quality of fresh strawberries (Fragaria ananassa cv Camarosa) under commercial storage conditions. LWT- Food Science and Technology, 52 (2): 80–92.
23. Ward G, Nussinovitch A, 1997. Characterizing the gloss properties of hydrocolloid films. Food Hydrocolloids, 11 (4): 357–365.
24. Yaman O, Bayındırlı L, 2002. Effects of an edible coating and cold storage on shelf-life and quality of cherries. LWT Food Science and Technology, 35 (2): 146-150.
25. Yaman O, Bayındırlı L, 2002. Effects of an edible coating and cold storage on shelf-life and quality of cherries. LWT Food Science and Technology, 35(2): 146-150.