Investigating the Effect of Yttrium Oxide Nanoparticle in U87MG Glioma and PC3 Prostate Cancer: Molecular Approaches

Year 2021, , 2307 - 2318, 01.09.2021

Elanur Aydın Karataş , Kübra Bayındırlı Özlem Özdemir Tozlu , Erdal Sönmez Süleyman Kerli , Hasan Türkez , Ayşenur Yazıcı

https://doi.org/10.21597/jist.872394

Cited By: 1

Abstract

Yttrium oxide (Y2O3) nanoparticles have very wide application areas such as biological
imaging, photodynamic therapy, the material sciences, in the chemical synthesis of inorganic
compounds, additives in plastic, paint, steel, optics, and iron. Potential risks to human health and the
environment should be evaluated in a multi-dimensional perspective when developing nanoparticles for
those applications. Therefore, in this research, we aimed to investigate changes in gene expression
profiles (genes involved in different biological pathways) influenced by commonly Yttrium oxide
(Y2O3) nanoparticle in human U87MG glioma and PC3 prostate cancer cell lines in vitro. The study was
planned to be carried out in two stages. In the first stage, cell viability and cytotoxicity parameters were
studied using 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide and lactate dehydrogenase
release assays, respectively, with human U87MG glioma and human PC3 prostate cancer cell cultures.
In the second stage, to obtain a clear insight into the molecular events after exposing, we examined the
effects of selected Y2O3 nanoparticle on the expression of genes in U87MG and PC3 cell cultures using
RT2 Profiler PCR Arrays. Y2O3 nanoparticles have IC20 of 0,18 mg/L and 2,903 mg/L in PC3 and
U87MG cell lines, respectively. Y2O3 nanoparticle induced up-regulation of 24 and down-regulation of
22 genes in PC3 cells and up-regulation of 53 and down-regulation of 27 genes in U87MG cells. This
study of gene expression profiles affected by nanotoxicity provides critical information for the clinical
and environmental applications of Y2O3 nanoparticles.

Keywords

Glioma, Prostate cancer cell, Cytotoxicity, In vitro gene expression, Nanotoxicity

Supporting Institution

Erzurum Teknik Üniversitesi

Project Number

2015/016

Thanks

This study was funded by Erzurum Technical University [grant numbers 2015/016].

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