Punica granatum L. Kabuklarinin Etanol Ekstraktlarının Sitotoksik ve Antibakteriyel Aktivitelerinin Değerlendirilmesi

Abstract

Çalışmada, nar kabuğu ekstraktlarının antibakteriyel aktivitesi ve güvenilirliğini kanıtlamak amacıyla insan dermal fibroblast primer hücreleri üzerindeki sitotoksisitesinin araştırılması amaçlanmıştır. Nar kabuklarının etanol özütlerinin antibakteriyel etkinliği Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus MRSA ATCC 67101, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus pneumoniae, Enterococcus faecalis, Staphylococcus epidermidis, Burkholderia cepacia, Bacillus cereus, Citrobacter freundii ve Cedecea neteri’ye karşı disk difüzyon ve mikrodilüsyon yöntemleriyle test edildi. Aynı zamanda, ekstraktın insan dermal fibroblast hücreleri üzerindeki etkileri de WST-8 deneyi ile belirlendi. Çalışmanın sonuçlarına göre; nar kabuklarının etanol özütü, A. baumannii, E. coli, S. aureus MRSA ATCC 67101, P. aeruginosa, E. faecalis, S. epidermidis, B. cepacia, B. cereus, C. freundii ve C. neteri’ye karşı 100 ila 500 μg mL-1 arasında değişen oranlarda MİK değerleri ile potansiyel olarak etkiliydi. Çalışmada ekstraktın K. pneumoniae, S. aureus and S. pneumoniae’ye karşı inhibisyon aktivitesi gözlenmedi. Aynı zamanda Punica granatum L. kabuğunun etanol özütü, dermal fibroblast hücrelerine karşı da herhangi bir sitotoksisiteye sahip değildi. Çalışmanın sonuçlarına göre, ekstraktın antibakteriyel özelliğe sahip olduğu ve toksik etkisi olmaması nedeniyle de birçok endüstriyel üründe doğal koruyucu bir bileşen olarak kullanılabileceği önerilmektedir.

Keywords

• Punica granatum L.
• nar kabuğu
• antibakteriyel aktivite
• sitotoksisite
• WST-8 test

The Evaluation of Cytotoxic and Antibacterial Activity of the Ethanol Extract of Punica granatum L. Peels

Abstract

In this study, it was aimed to investigate the antibacterial activity of pomegranate peel’s extract and the cytotoxicity on the human dermal fibroblast primary cells to rationalize the safe usage of this extract. The antibacterial efficiency of ethanol extract of pomegranate peels was evaluated against Acinetobacter baumannii, Escherichia coli, Staphylococcus aureus MRSA ATCC 67101, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa, Streptococcus pneumoniae, Enterococcus faecalis, Staphylococcus epidermidis, Burkholderia cepacia, Bacillus cereus, Citrobacter freundii and Cedecea neteri by disc diffusion and microdilution assays. At the same time, the effects of this extract on the human dermal fibroblast primary cells were determined by WST-8 assay. The ethanol extract of pomegranate peels was potentially effective with different efficiency against A. baumannii, E. coli, S. aureus MRSA ATCC 67101, P. aeruginosa, E. faecalis, S. epidermidis, B. cepacia, B. cereus, C. freundii, and C. neteri at MIC's ranged from 100 to 500 μg mL-1. However, it exhibits no inhibition activity against K. pneumoniae, S. aureus and S. pneumoniae. The ethanol extract of Punica granatum L. peel exhibited no cytotoxic activity against the normal human dermal fibroblast primary cells. According to results it may be suggested that this extract possess antibacterial properties and the safety, and therefore, it can be used as a natural preservative ingredient in many industrial products.

Keywords

• Punica granatum L.
• pomegranate peels
• antibacterial activity
• cytotoxicity
• WST-8 assay

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