Arıcılıkta Bazı Biyoteknolojik Gelişmelere Bakış

Year 2021, , 3308 - 3323, 15.12.2021

Erkan Topal , Aybike Sarıoğlu , Devrim Oskay , Ralica Balkanska , Nazmiye Güneş , Rahşan İvgin Tunca

https://doi.org/10.21597/jist.888950

Abstract

Arıcılıktaki araştırmalar birçok bilim dalının ışığında yapılmakta olup biyoteknoloji bunlardan biridir. İklimde yaşanan değişimler, arıların beslenme ve besin kaynaklarını etkilerken, son yıllarda hastalık ve zararlıların giderek artması ve kimyasal ilaçlara karşı gerek arının gerekse de parazit ve patojenlerin direnç geliştirmeleri sebebiyle hastalık etmenlerine bu kimyasalların çare olamaması arıcılıkta biyoteknolojik çalışmalara yönelimi hızlandırmıştır. Hastalıklara dirençli / verimli arı ırklarının ve mevcut katkı maddelerinin geliştirilmesiyle birlikte, yeni yem kaynakları oluşturulmasına yönelik biyoteknolojik araştırmalar yapılmaktadır. Özellikle dünyadaki gelişmelere uyum sağlamak sürdürülebilir arıcılık faaliyetleri açısından da oldukça önem taşımaktadır. Bu çalışmada bal arısı ve arıcılık sektöründe yapılmış bazı biyoteknolojik araştırmalar incelenerek bu alandaki gelişmelerin ortaya konulması ve yakın gelecekte yapılacak çalışmalara ışık tutulması amaçlanmıştır.

Keywords

Bal arısı, Mikroorganizma, Arı sağlığı, Biyokimya

A Summary of Some Recent Biotechological Advancements in Beekeeping

Abstract

Research in beekeeping is carried out in the light of many branches of science, and biotechnology is one of them. While the changes in the climate affect the nutrition and food resources of the bees, the increase of diseases and pests in recent years and the resistance of both bees and parasites and pathogens against chemical pesticides has accelerated the orientation to biotechnological studies in beekeeping. Many studies are being carried out to develop resistant / efficient bee breeds to improve existing additives and to create new food resources. Especially adapting to the developments in the world is essential to sustainable beekeeping activities. This review aims to reveal the developments in this field and shed light on the studies in the near future by examining some biotechnological research conducted in the honey bee and beekeeping sector.

Keywords

Honey bee, microorganism, bee health, biochemistry

References

• Aizen MA, Garibaldi LA, Cunningham SA, Klein AM, 2008. Long-term global trends in crop yield and production reveal no current pollination shortage but increasing pollinator dependency. Current Biology, 18:1572–1575.
• Alberoni D, Baffoni L, Gaggìa F, Ryan PM, Murphy K, Ross PR, Di Gioia D, 2018. Impact of beneficial bacteria supplementation on the gut microbiota, colony development and productivity of Apis mellifera L. Beneficial microbes, 9(2), 269-278.
• Alcay S, Cakmak S, Cakmak I, Mulkpinar E, Gokce E, Ustuner B, Nur Z, 2019a. Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders. Cryobiology, 87, 28-31.
• Alcay S, Cakmak S, Cakmak I, Mülkpınar E, Toker MB, Üstüner B, Nur, Z, 2019b. Drone Semen Cryopreservation with Protein Supplemented TL-Hepes Based Extender. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 25(4).
• Alcay S, Ustuner B, Çakmak I, Çakmak SS, Nur Z, 2015. Effects of various cryoprotective agents on post-thaw drone semen quality. Kafkas Universitesi Veteriner Fakultesi Dergisi, 21; 31-35.
• Alpan O, 1989. Biyoteknoloji Ve Hayvan Islahı. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 29(1), 107-114.
• Anderson D, Trueman J, 2000. Varroa jacobsoni (Acari: Varroidae) is more than one species. Experimental & Applied Acarology, 24:165–189.
• Anklam E, 1998. A review of the analytical methods to determine the geographical and botanical origin of honey. Food Chemistryistry, 63(4): 549-562.
• Aumeier P, Rosenkranz P, 2001. Scent or movement of Varroa destructor mites does not elicit hygienic behaviour by Africanized and Carniolan honey bees. Apidologie, 32: 253–264.
• Annoscia D, Del Piccolo F, Nazzi F, 2012. How does the mite Varroa destructor kill the honeybee Apis mellifera? Alteration of cuticular hydrcarbons and water loss in infested honeybees. Journal of Insect Physiology, 58:1548–1555.
• Avalos A, Fang M, Pan H, Lluch AR, Lipka AE, Zhao SD, Hudson ME, 2020. Genomic regions influencing aggressive behavior in honey bees are defined by colony allele frequencies. Proceedings of the National Academy of Sciences, 117(29), 17135-17141.
• Bąk B, Wilde J, Siuda M, 2010. Comparison of hygienic behaviour between five honey bee breeding lines. Journal of Apicultural Science, 54(2), 17-24.
• Blomquist GJ, Bagnères AG, 2010. Insect hydrocarbons: biology, biochemistry, and chemical ecology. (Cambridge University Press).
• Boecking O, Drescher W,1992. The removal response of Apis mellifera L. colonies to brood in wax and plastic cells after artificial and natural infestation with Varroa jacobsoni Oud. and to freeze-killed brood. Experimental & Applied Acarology, 16:321–329.
• Braga AR, Gomes DG, Rogers R, Hassler EE, Freitas BM, Cazier JA, 2020. A method for mining combined data from in-hive sensors, weather and apiary inspections to forecast the health status of honey bee colonies. Computers and Electronics in Agriculture, 169, 105161.
• Cánovas F, de la Rúa P, Serrano J, Galián J, 2011. Microsatellite variability reveals beekeeping influences on Iberian honeybee populations, Apidologie, 42(3), 235-251.
• Chakrabarti P, Lucas HM, Sagili RR, 2020. Evaluating Effects of a Critical Micronutrient (24-Methylenecholesterol) on Honey Bee Physiology. Annals of the Entomological Society of America, 113(3), 176-182.
• Chapman NC, Lim J, Oldroyd BP, 2008. Population genetics of commercial and feral honey bees in Western Australia. Journal of economic entomology, 101(2), 272-277.
• Cobey SW, Tarpy DR, Woyke J, 2013. Standard methods for instrumental insemination of Apis mellifera queens. Journal of Apicultural Research, 52(4), 1-18.
• Cremer S, Armitage SA, Schmid-Hempel P, 2007. Social immunity. Current Biology 17, R693–R702
• Çetiner S, 2011. Biyogüvenlik nedir? ne değildir? Tarla Sera Dergisi, 88-91.
• Dadkhah F, Nehzati-Paghaleh G, Zhandi M, Emamverdi M, Hopkins BK, 2016. Preservation of honey bee spermatozoa using egg yolk and soybean lecithin-based semen extenders and a modified cryopreservation protocol. Journal of Apicultural Research, 55(4), 279-283.
• Dietemann V, Pflugfelder J, Anderson D, Charrière JD, Chejanovsky N, Dainat B, Neumann P, 2012. Varroa destructor: research avenues towards sustainable control. Journal of Apicultural Research, 51(1), 125-132.
• Di Gioia D, Biavati B, 2018. Probiotics and prebiotics in animal health and food safety: Conclusive remarks and future perspectives. In Probiotics and prebiotics in animal health and food safety (pp. 269-273). Springer, Cham.
• Erçetin Ş, 1999. Biyoteknoloji ile Değişen Dünya Düzeni ve Eğitim-1. Kuram ve Uygulamada Egitim Yönetimi Dergisi, 5(2), 169-180.
• Fanciotti MN, Tejerina M, Benítez-Ahrendts MR, Audisio, MC, 2018. Honey yield of different commercial apiaries treated with Lactobacillus salivarius A3iob, a new bee-probiotic strain. Beneficial microbes, 9(2), 291-298.
• Francis B, Blanton W, Nunamaker R, 1985. Extractable surface hydrocarbons of workers and drones of the genus Apis. Journal of Apicultural Research 24, 13–26.
• Gąbka J, Cobey SW, 2018. Factors, based on common practices, affecting the results of instrumental insemination of honey bee queens. Apidologie, 49(6), 773-780.
• Galbraith DA, Fuller ZL, Ray AM, Brockmann A, Frazier M, Gikungu MW, Grozinger CM, 2018. Investigating the viral ecology of global bee communities with high-throughput metagenomics. Scientific reports, 8(1), 1-11.
• Galbraith DA, Yang X, Nino EL, Yi S, Grozinger C, 2015. Parallel epigenomic and transcriptomic responses to viral infection in honey bees (Apis mellifera). PLoS Pathog, 11(3), e1004713
• Gebremedhn H, Deboutte W, Schoonvaere K, Demaeght P, De Smet L, Amssalu B, de Graaf DC, 2020. Metagenomic Approach with the NetoVIR Enrichment Protocol Reveals Virus Diversity within Ethiopian Honey Bees (Apis mellifera simensis). Viruses, 12(11), 1218.
• Gibbs AG,1998. Water-proofing properties of cuticular lipids. American Zoologist, 38: 471–482.
• Gül ÜD, 2014. Sağlık alanında biyoteknolojik uygulamalar: Kırmızı biyoteknoloji. Bilecik Şeyh Edebali Üniversitesi Fen Bilimleri Dergisi, 1(1), 66-70.
• Gül A, Ceylan DA, 2016. Bal Arısı (Apis Mellifera L.) Spermasının Taze Ve Dondurularak Muhafaza Edilmesi. Uludag Bee Journal, 16(2): 76-84
• Gül A, Şahinler N, Onal AG, Hopkins BK, Sheppard WS, 2017. Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility. Theriogenology, 101, 109-113.
• Gramacho KP, Spivak M, 2003. Differences in olfactory sensitivity and behavioral responses among honey bees bred for hygienic behavior. Behavioral Ecology and Sociobiology, 54: 472–479.
• Grozinger CM, Zayed A, 2020. Improving bee health through genomics. Nature Reviews Genetics, 1-15.
• Harbo JR, 1977. Survival of honey bee spermatozoa in liquid nitrogen. Annals of the entomological society of America, 70(2), 257-258.
• Hawkins J, de Vere N, Griffith A, Ford CR, Allainguillaume J, Hegarty MJ, Baillie L, AdamsGroom B, 2015. Using DNA metabarcoding to identify the floral composition of honey: A new tool for investigating honey bee foraging preferences. PLoS One, 10(8), e0134735.
• Harris JW, 2007. Bees with Varroa sensitive hygiene preferentially remove mite infested pupae aged ≤ fve days post capping. Journal of Apicultural Research, 46: 134–139.
• He XJ, Zhang XC, Jiang WJ, Barron AB, Zhang JH, Zeng ZJ, 2016. Starving honey bee (Apis mellifera) larvae signal pheromonally to worker bees. Scientific reports 6, 22359.
• Hefetz A, 2007. The evolution of hydrocarbon pheromone parsimony in ants (Hymenoptera: Formicidae)—interplay of colony odor uniformity and odor idiosyncrasy. Myrmecological News ,10: 59–68.
• Hopkins BK, Herr C, 2010. Factors affecting the successful cryopreservation of honey bee (Apis mellifera) spermatozoa. Apidologie, 41(5), 548-556.
• Huang Q, 2013. Genetic analysis of Nosema tolerance in the honey bee, Apis mellifera. Doctoral Thesis. Universitäts- und Landesbibliothek Sachsen-Anhalt. http://dx.doi.org/10.25673/938. (Erişim Tarihi: 30.11.2020).
• Huang Q, Kryger P, Le Conte Y, Moritz RFA, 2012. Survival and immune response of drones of a Nosemosis tolerant honey bee strain towards N. ceranae infections. J Invertebr Pathol.109(3):297–302. pmid:22285444
• İnanç M, Daşkın A, 2015. Sığırlarda suni tohumlama uygulamaları yönünden genomik seleksiyonun önemi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 10(2);139-145.
• Jain SA, De Jesus FT, Marchıoro GM, De Araújo ED, 2013. Extraction of DNA from honey and its amplification by PCR for botanical identification. Food Sci. Technol, Campinas, 33(4): 753-756.
• Kayıhan C, 2018. Biyoteknoloji: On Bin Yıllık Serüven. Pivolka, Nisan 2018, Cilt: 8, Sayı: 27
• Kearns CA, Inouye DW, Waser NM, 1998. Endangered mutualisms: the conservation of plant-pollinator interactions. Annual Review of Ecology and Systematics, 83-112.
• Kekeçoğlu M, 2015. Yığılca balarısı (apis mellifera L.)’nın hijyenik davranış bakımından performanslarının belirlenmesi ve geliştirilmesi. Uludağ Arıcılık Dergisi, 15(2), 47-59.
• Khansaritoreh E, Salmaki Y, Ramezani E, Azirani TA, Keller A, Neumann K, Alizadeh K, Zarre S, Beckh G, Behling H, 2020. Employing DNA metabarcoding to determine the geographical origin of honey. Heliyon, 6, 11, 1 – 6.
• Kirrane MJ, de Guzman LI, Holloway B, Frake AM, Rinderer TE, Whelan PM, 2015. Phenotypic and genetic analyses of the varroa sensitive hygienic trait in Russian honey bee (Hymenoptera: Apidae) colonies. PLoS One, 10(4), e0116672.
• Kolankaya N, 2000. “Küreselleşme Sürecinde Biyoteknoloji ve Biyogüvenlik'', Biyoteknolojiye Bir Bakış: Dünya ve Türkiye Sempozyum Bildirileri Kitabı, T.C. Çevre Bakanlığı, T.C. Tarım ve Köyişleri Bakanlığı ve Biyoteknoloji Derneği Ortak Yayınları, Ankara, pp. 1- 6,
• Kovačić M, Raguž N, Majić I, Lukić B, Sarajlić A, Puškadija Z, 2020. Results of the first generation of Carniolan honey bees (Apis mellifera carnica) selection to Varroa destructor resistant traits. sa55, 334.
• Koru BY, 2018. Bal Arılarında (Apis Mellifera) Beslenme Farklılığının Yaşam Uzunluğu, Gelişme, Davranış (Amılp-1, Vg) Ve Nörotransmitter Salınımını Düzenleyen (Brp) Genlerindeki Etkilerinin Araştırılması. Namık Kemal Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi. Tekirdağ. 65 sayfa.
• Kulhanek K, Steinhauer N, Rennich K, Caron DM, Sagili RR, Pettis JS, vanEngelsdorp D, 2017. A national survey of managed honey bee 2015–2016 annual colony losses in the USA. Journal of Apicultural Research, 56(4), 328-340.
• Kunc M, Dobeš P, Hurychová J, Vojtek L, Poiani SB, Danihlík J, Hyršl P, 2019. The Year of the Honey Bee (Apis mellifera L.) with Respect to Its Physiology and Immunity: A Search for Biochemical Markers of Longevity. Insects, 10(8), 244.
• Kurze C, Le Conte Y, Dussaubat C, Erler S, Kryger P, Lewkowski O, Moritz RF, 2015. Nosema tolerant honeybees (Apis mellifera) escape parasitic manipulation of apoptosis. PLoS One, 10(10), e0140174.
• Lazarov S, Stoyanov I, Georgıeva V, Zhelyazkova I, Ivanova E, 2019. Aloenzimna genetichna harakteristika na pchelni semeystva Apis mellifera (Hymenoptera: Apidae) ot Bulgaria s razlichno higienno povedeni. Journal of Central European Agriculture, 20(2), 592-597.
• Lee KV, Steinhauer N, Rennich K, Wilson ME, Tarpy DR, Caron DM, Sagili R, 2015. A national survey of managed honey bee 2013–2014 annual colony losses in the USA. Apidologie, 46(3), 292-305.
• Lee S, Lim S, Choi YS, Lee ML, Kwon HW, 2020. Volatile disease markers of American foulbrood-infected larvae in Apis mellifera. Journal of insect physiology, 122, 104040.
• Leonard SP, Powell JE, Perutka J, Geng P, Heckmann LC, Horak RD, Moran NA, 2020. Engineered symbionts activate honey bee immunity and limit pathogens. Science, 367(6477), 573-576.
• Li-Byarlay H, Boncristiani H, Howell G, Herman J, Clark L, Strand MK, Rueppell O, 2020. Transcriptomic and epigenomic dynamics of honey bees in response to lethal viral infection. Frontiers in genetics, 11, 1056.
• Locke B, Low M, Forsgren E, 2019. An integrated management strategy to prevent outbreaks and eliminate infection pressure of American foulbrood disease in a commercial beekeeping operation. Preventive Veterinary Medicine, 167, 48-52.
• Lodesani M, Franceschetti S, Dall’Ollio R, 2019. Evaluation of early spring bio-technical management techniques to control varroosis in Apis mellifera. Apidologie, 50(2), 131-140.
• Maleszka R, 2008. Epigenetic integration of environmental and genomic signals in honey bees: the critical interplay of nutritional, brain and reproductive networks. Epigenetics, 3(4), 188-192.
• McAfee A, Chapman A, Iovinella I, Gallagher-Kurtzke Y, Collins TF, Higo H, Foster LJ, 2018. A death pheromone, oleic acid, triggers hygienic behavior in honey bees (Apis mellifera L.). Scientific Reports 8, 5719.
• Mills S, 2019. Instrumental Insemination and Semen Cryopreservation: Can Honey Bees be Selectively Bred Like Cattle? Journal of the NACAA, 12(2).
• Möckel N, Gisder S, Genersch E, 2011. Horizontal transmission of deformed wing virus: pathological consequences in adult bees (Apis mellifera) depend on the transmission route. Journal of General Virology 92, 370–377.
• Nawrocka A, Kandemir İ, Fuchs S, Tofilski A, 2018. Computer software for identification of honey bee subspecies and evolutionary lineages. Apidologie, 49(2), 172-184.
• Nazzi F, Della Vedova G, D’Agaro M, 2004. A semiochemical from brood cells infested by Varroa destructor triggers hygienic behaviour in Apis mellifera. Apidologie, 35: 65–70.
• Nur Z, Seven Çakmak S, Çakmak İ, Onder NT, Gokçe E, Ustuner B, Alcay S, Toker MB, Soylu MK, 2020. Effects of trehalose supplementation on post-thaw sperm quality of honey bee drones. Online J. Anim. Feed Res., 10(5): 191-196
• O'Brien C, Arathi HS, 2018. Bee genera, diversity and abundance in genetically modified canola fields. GM crops & food, 9(1), 31-38.
• Oskay D, Kükrer M, Kence A, 2019. Muğla Bal Arısında (Apis mellifera anatoliaca) Amerikan Yavru Çürüklüğü Hastalığına Karşı Direnç Geliştirilmesi. Arıcılık Araştırma Dergisi, 11(1), 8-20.
• Özcan BD, Ayaşan T, 2009. Hayvan Beslemede Biyoteknoloji Uygulamaları. Tavukçuluk Araştırma Dergisi, 8(1), 58-63.
• Paillard M, Rousseau A, Giovenazzo P, Bailey JL, 2017. Preservation of domesticated honey bee (hymenoptera: apidae) drone semen. Journal of Economic Entomology, 110(4), 1412-1418.
• Patruica S, Dumitrescu G, Stancu A, Bura M, Dunea IB, 2012.The Effect of Prebiotic and Probiotic Feed Supplementation on the Wax Glands of Worker Bees (Apis mellifera). Scientific Papers: Animal Sciences and Biotechnologies, 45 (2), 268 – 271.
• Patruica S, Dumitrescu G, Popescu R, Filimon NM, 2013. The effect of prebiotic and probiotic products used in feed to stimulate the bee colony (Apis mellifera) on intestines of working bees. Journal of Food, Agriculture & Environment, 11(3&4), 2461-2464.
• Petersen GEL, Fennessy PF, Amer PR, Dearden PK, 2020. Designing and implementing a genetic improvement program in commercial beekeeping operations. Journal of Apicultural Research, 1-10. Pinheiro PV, de Faria JC, 2020. GMOs–Impact on Non-target Arthropods. GMOs, 87-127.
• Prosser SWJ, Hebert PDN, 2017. Rapid identification of the botanical and entomological sources of honey using DNA metabarcoding. Food Chemistry, 214: 183-191.
• Ptaszyńska AA, Borsuk G, Zdybicka-Barabas A, Cytryńska M, Małek W, 2016. Are commercial probiotics and prebiotics effective in the treatment and prevention of honeybee nosemosis C?. Parasitology research, 115(1), 397-406.
• Regan T, Barnett MW, Laetsch DR, Bush SJ, Wragg D, Budge GE, Freeman TC, 2018. Characterisation of the British honey bee metagenome. Nature communications, 9(1), 1-13.
• Ricigliano VA, 2020. Microalgae as a promising and sustainable nutrition source for managed honey bees. Archives of Insect Biochemistry and Physiology, 104(1), e21658
• Rittschof CC, Rubin BE, Palmer JH, 2019. The transcriptomic signature of low aggression in honey bees resembles a response to infection. BMC genomics, 20(1), 1-14.
• Rollo C, Czvzewska E, Borden J,1994. Fatty acid necromones for cockroaches. Naturwissenschaften 81, 409–410.
• Rosenkranz P, Aumeier P, Ziegelmann B, 2010. Biology and control of Varroa destructor. Journal of Invertebrate Pathology 103, S96–S119.
• Ruane J, Sonnino A, 2011. Agricultural biotechnologies in developing countries and their possible contribution to food security. Journal of Biotechnology, 156 (4), 356-363
• Saelao P, Simone-Finstrom M, Avalos A, Bilodeau L, Danka R, de Guzman L, Tokarz P, 2020. Genome-wide patterns of differentiation within and among US commercial honey bee stocks. BMC genomics, 21(1), 1-12.
• Sağırkaya H, 2009. Sığırlarda embriyo transfer uygulaması ve Türkiye açısından önemi. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 28(2), 11-20.
• Salvy M, Martin C, Bagneres AG, Provost É, Roux M, Le Conte Y, Clement J L, 2001. Modifications of the cuticular hydrocarbon profile of Apis mellifera worker bees in the presence of the ectoparasitic mite Varroa jacobsoni in brood cells. Parasitology 122, 145–159.
• Sammataro D, Untalan P, Guerrero F, Finley J, 2005. The resistance of Varroa mites (Acari: Varroidae) to acaricides and the presence of esterase. International Journal of Acarology, 31: 67–74
• Scott K, 1998. Handbook of Industrial Membranes, 2 nd ed., 14, pp. 655- 680
• Seitz N, Traynor KS, Steinhauer N, Rennich K, Wilson ME, Ellis JD, Rose R, Tarpy DR, Sagili RR, Caron DM, Delaplane KS, Rangel J, Lee K, Baylis K, Wilkes JT, Skinner JA, Pettis JS, vanEngelsdorp D, 2016. A national survey of managed honey bee 2014–2015 annual colony losses in the USA, Journal of Apicultural Research, DOI: 10.1080/00218839.2016.1153294.
• Schöning C, Gisder S, Geiselhardt S, Kretschmann I, Bienefeld K, Hilker M, Genersch E, 2012. Evidence for damage-dependent hygienic behaviour towards Varroa destructor-parasitised brood in the western honey bee, Apis mellifera. Journal of Experimental Biology, 215(2), 264-271.
• Schulte C, Theilenberg E, Müller-Borg M, Gempe T, Beye M, 2014. Highly efficient integration and expression of piggyBac-derived cassettes in the honeybee (Apis mellifera). Proceedings of the National Academy of Sciences, 111(24), 9003-9008.
• Schmehl DR, Teal PE, Frazier JL, Grozinger CM, 2014. Genomic analysis of the interaction between pesticide exposure and nutrition in honey bees (Apis mellifera). Journal of Insect Physiology, 71, 177-190.
• Shpigler HY, Saul MC, Murdoch EE, Cash‐Ahmed AC, Seward CH, Sloofman L, Robinson GE, 2017. Behavioral, transcriptomic and epigenetic responses to social challenge in honey bees. Genes, Brain and Behavior, 16(6), 579-591.
• Spötter A, Gupta P, Nürnberg G, Reinsch N, Bienefeld K, 2012. Development of a 44K SNP assay focussing on the analysis of a varroa‐specific defence behaviour in honey bees (Apis mellifera carnica). Molecular Ecology Resources, 12, 323–332.
• Spötter A, Gupta P, Mayer M, Reinsch N, Bienefeld K, 2016. Genome-wide association study of a Varroa-specific defense behavior in honeybees (Apis mellifera). Journal of Heredity, 107(3), 220-227
• Spivak M, Downey DL, 1998. Field assays for hygienic behavior in honey bees (Hymenoptera: Apidae). Journal of Economic Entomology, 91: 64–70.
• Spivak M, Reuter GS, 2001. Resistance to American foulbrood disease by honey bee colonies Apis mellifera bred for hygienic behavior. Apidologie. 32:555–65.
• Steinhauer NA, Rennich K, Wilson ME, Caron DM, Lengerich EJ, Pettis JS, 2014. Bee Informed Partnership. A national survey of managed honey bee 2012–2013 annual colony losses in the USA: results from the Bee Informed Partnership. Journal of Apicultural Research, 53(1), 1-18.
• Soares S, Grazina L, Costa J, Amaral JS, Oliveira B, Mafra I, 2018. Botanical authentication of lavender (Lavandula spp.) honey by a novel DNA-barcoding approach coupled to high resolution melting analysis. Food Control, 86: 367-373.
• Su TS, Wu CC, Chang LW, 2020. Integration of audio surveillance on a queen bee rearing and breeding management system. Journal of Information and Optimization Sciences, 1-16.
• Syromyatnikov MY, Borodachev AV, Kokina AV, Popov VN, 2018. A molecular method for the identification of honey bee subspecies used by beekeepers in Russia. Insects, 9(1), 10.
• Tarpy, DR, Nıelsen DI, 2002. Sampling error, effective paternity, and estimating the genetic structure of honey bee colonies (Hymenoptera: Apidae). Annals of the Entomological Society of America 95: 513–528.
• Taylor MA, Guzman-Novoa E, Morfin N, Buhr MM, 2009. Improving viability of cryopreserved honey bee (Apis mellifera L.) sperm with selected diluents, cryoprotectants, and semen dilution ratios. Theriogenology, 72(2), 149-159.
• Tlak Gajger I, Vlainić J, Šoštarić P, Prešern J, Bubnič J, Smodiš ŠMI, 2020. Effects on Some Therapeutical, Biochemical, and Immunological Parameters of Honey Bee (Apis mellifera) Exposed to Probiotic Treatments, in Field and Laboratory Conditions. Insects, 11(9), 638.
• Tola YH, Waweru JW, Hurst GD, Slippers B, Paredes JC, 2020. Characterization of the Kenyan Honey Bee (Apis mellifera) Gut Microbiota: A First Look at Tropical and Sub-Saharan African Bee Associated Microbiomes. Microorganisms, 8(11), 1721.
• Utzeri VJ, Ribani A, Fontanesi L, 2018. Authentication of honey based on a DNA method to differentiate Apis mellifera subspecies: Application to Sicilian honey bee (A. m. siciliana) and Iberian honey bee (A. m. iberiensis) honeys. Food Control, 91, 294-301.
• Watson JD, Crick FH, 1953. The structure of DNA. In Cold Spring Harbor symposia on quantitative biology (Vol. 18, pp. 123-131). Cold Spring Harbor Laboratory Press.
• Wang H, Liu C, Liu Z, Wang Y, Ma L, Xu B, 2020. The different dietary sugars modulate the composition of the gut microbiota in honeybee during overwintering. BMC microbiology, 20(1), 1-14.
• Wagoner K, Spivak M, Hefetz A, Reams T, Rueppell O, 2019. Stock-specific chemical brood signals are induced by Varroa and Deformed Wing Virus, and elicit hygienic response in the honey bee. Scientific reports, 9(1), 1-14.
• Wagoner KM, Millar JG, Schal C, Rueppell O, 2020. Cuticular pheromones stimulate hygienic behavior in the honey bee (Apis mellifera). Scientific Reports, 10(1). doi:10.1038/s41598-020-64144-8. Scientific Reports, 9 (1). doi:10.1038/s41598-019-45008-2.
• Wegener J, May T, Kamp G, Bienefeld K, 2014a. New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen. Journal of Economic Entomology, 107(1), 47-53.
• Wegener J, May T, Kamp G, Bienefeld K, 2014b. A successful new approach to honeybee semen cryopreservation. Cryobiology, 69(2), 236-242.
• Wilfert L, Long G, Leggett HC, Schmid-Hempel P, Butlin R, Martin SJM, Boots M, 2016. Deformed wing virus is a recent global epidemic in honeybees driven by Varroa mites. Science 351, 594–597
• Wilson EO, Durlach NI, Roth LM, 1958. Chemical releasers of necrophoric behavior in ants. Psyche 65, 108–114.
• Winston ML, 2003. Bees and biotechnology. Bee World, 84(4), 141-143
• Woyke J, 1962. Natural and artificial insemination of queen honeybees. Bee World, 43(1), 21-25.
• Wu X, Galbraith DA, Chatterjee P, Jeong H, Grozinger CM, Yi SV, 2020. Lineage and parent-of-origin effects in DNA methylation of honey bees (Apis mellifera) revealed by reciprocal crosses and whole-genome bisulfite sequencing. Genome Biology and Evolution, 12(8), 1482-1492.
• van Alphen JJ, Fernhout BJ, 2020. Natural selection, selective breeding, and the evolution of resistance of honeybees (Apis mellifera) against Varroa. Zoological Letters, 6, 1-20.
• vanEnglesdorp D, Hayes JJr, Underwood RM, Pettis J, 2008. A survey of honey bee colony losses in the US, Fall 2007 to Spring 2008. PloS one 3, e4071.
• Villa JD, Danka RG, Harris JW, 2009. Simplified methods of evaluating colonies for levels of Varroa Sensitive Hygiene (VSH). Journal of Apicultural Research and Bee World, 48(3): 162–167.
• Yeşilbağ D, 2004. Tarımsal ve hayvansal ürünlerde modern biyoteknoloji ve organik üretim. Uludag Univ. J. Fac. Vet. Med, 23(1-3), 157-162.
• Yıldız MA, Gürel F, Özkan M, 2010. Türkiye Bal Arısı Populasyonlarının Mikrosatelit DNA Analizi Yöntemiyle Tanımlanması, Scientific and Technical Research Council of Turkey, Project No: 108O200
• Yuanyuan J, Yi J, Yongmin L, Xuan Z, Xiaoyun C, Junfeng X, Xiaoli X, 2019. Potential effect of genetically modified maize DR 12-5 on honey bee (Apis mellifera). Acta Agriculturae Zhejiangensis, 31(11), 1834.