Quantification of The Expression Level of Some Drought Stress-Related Genes in Wheat (Triticum aestivum L.) Treated With Plant Growth Promoting Rhizobacteria

Abstract

Bread wheat (Triticum aestivum L.) is one of the most important plant species in terms of sustainable agriculture, as it is a basic food source and a product that can be produced all over the world. The fact that the wheat plant has a more complex genetic structure than corn, paddy and all other agricultural products makes the breeding of this species a difficult and time-consuming process. On the other hand, it is possible to bring the yield values to the desired points by understanding the reactions of wheat to environmental factors. In this study, the relationship of resistance to drought and diseases, which are the most common problems in wheat breeding, with PGPB (Plant Growth Promoting Bacteria), which has ACC deaminase effect, was investigated. In our study, the effect of ACC deaminase-synthesizing bacteria on two different bread wheat varieties (Need 79, Bezostaja 1) and in drought conditions was investigated. In this study, expression levels of some transcription factors involved in drought resistance mechanism with ACC deaminase effect were measured by q-RT PCR. In addition, the glutathione reductase level was measured in both wheat genotypes and its relationship with the genes was revealed. Although the data obtained as a result of the study have varying degrees of activity, it has been found that PGPB bacteria have a reducing effect on the negative effects of drought stress.

Keywords

• Rhizobacteria
• wheat
• ACC deaminase
• gene expression
• transcription factor
• glutathion

Project Number

2017-SİÜZİR-63

Bitki Gelişimini Tetikleyen Rizobakterilerin Uygulandığı Buğdayda (Triticum aestivum L.) Kuraklık Stresi ile İlişkili Bazı Genlerin İfade Seviyesinin Ölçülmesi

Abstract

Ekmeklik buğday (Triticum aestivum L.) temel besin kaynağı olması ve tüm dünyada üretimi yapılabilen bir ürün olması nedeniyle sürdürülebilir tarım açısından en önemli bitki türlerinden biridir. Buğday bitkisinin genetik yapı olarak mısır, çeltik ve diğer tüm tarımsal ürünlerden daha kompleks bir yapıya sahip olması bu türün ıslahını zor ve uzun zaman alan bir süreç haline getirmektedir. Diğer taraftan verim değerlerinin istenilen noktalara getirilebilmesi buğdayın çevresel faktörlere verdiği tepkilerin anlaşılması ile mümkün olabilmektedir. Bu çalışmada da buğday ıslahında en sık karşılaşılan sorunlardan kuraklık ve hastalıklara karşı direncin ACC deaminaz etkisi gösteren PGPB (Plant Growth Promoting Bacteria) ile ilişkisi incelenmiştir. Çalışmamızda ACC deaminaz sentezleyen bakterilerin iki farklı ekmeklik buğday çeşidinde (Gerek 79, Bezostaja 1) ve kuraklık koşullarındaki etkisi incelenmiştir. Çalışma ile ACC deaminaz etkisi ile kuraklığa karşı dayanıklılık mekanizmasında rol alan bazı transkripsiyon faktörlerin ifade seviyeleri q-RT PCR ile ölçülmüştür. Ayrıca her iki buğday genotipinde glutatyon redüktaz seviyesi ölçülerek genler ile olan ilişkisi ortaya konulmuştur. Çalışma sonucunda elde edilen veriler değişen etkinlik derecesine sahip olmakla birlikte PGPB bakterilerinin kuraklık stresinin olumsuz etkilerini azaltıcı etkiye sahip olduğu bulunmuştur.

Keywords

• Kuraklık
• buğday
• ACC deaminaz
• gen ifadesi
• transkripsiyon faktör
• glutatyon

Supporting Institution

Siirt Üniversitesi Bilimsel Araştırmalar Proje Birimi

Project Number

2017-SİÜZİR-63

Thanks

Bu çalışma Siirt Üniversitesi Bilimsel Araştımalar Proje (BAP) birimi tarafından 2017-SİÜZİR-63 nolu proje kapsamında desteklenmiştir.

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