Research Article
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Year 2021, , 3119 - 3127, 15.12.2021
https://doi.org/10.21597/jist.911056

Abstract

Supporting Institution

Kafkas Üniversitesi Bilimsel Araştırma Projeleri (BAP) Koordinatörlüğü

Project Number

2018-FM-16

Thanks

Erzurum Teknik Üniversitesi Yüksek Teknoloji Uygulama ve Araştırma Merkezi (YUTAM)

References

  • Abbasov M, Akparov Z, Gross T, Babayeva S, Izzatullayeva V, Hajiyev E, Rustamov K, Gross P, Tekin M, Akar T, Chao S, Brueggeman R, 2018. Genetic Relationship of Diploid Wheat (Triticum spp.) Species Assessed by SSR Markers. Genetic Resources and Crop Evolution; 65(5):1441-1453.
  • Akfirat FS, Uncuoglu AA, 2013. Genetic Diversity of Winter Wheat (Triticum aestivum L.) Revealed by SSR Markers. Biochemical Genetics, 51:223-229. DOI: 10.1007/s10528-012-9557-6.
  • Arystanbekkyzy M, Nadeem MA, Aktaş H, Yeken MZ, Zencirci N, Nawaz MA, Ali F, Haider MS, Tunc K, Chung G, Baloch FS, 2018. Phylogenetic and Taxonomic Relationship of Turkish Wild and Cultivated Emmer (Triticum turgidum ssp. dicoccoides) Revealed by IPBS-Retrotransposons Markers. International Journal of Agriculture and Biology, 21(1): 155-163.
  • Belete YS, Shimelis H, Laing MK, Mathew I, 2020. Genetic Diversity and Population Structure of Bread Wheat Genotypes Determined via Phenotypic and SSR Marker Analyses under Drought-Stress Conditions. Journal of Crop Improvement, doi: 10.1080/15427528.2020.1818342.
  • Chen X, Min D, Yasir TA, Hu Y-G, 2012. Genetic Diversity, Population Structure and Linkage Disequilibrium in Elite Chinese Winter Wheat Investigated with SSR Markers. PLoS One 7(9): e44510.
  • Demirel F, 2020. Genetic Diversity of Emmer Wheats Using IPBS-Retrotranspozon Markers. European Journal of Science and Technology, 20, 640-646.
  • Dong P, Wei Y-M, Chen G-Y, Li W, Wang J-R, Nevo E, Zheng Y-L, 2009. EST-SSR Diversity Correlated with Ecological and Genetic Factors of Wild Emmer in Israel. Hereditas 146: 1-10.
  • Doyle JJ, Doyle JL, 1990. Isolation of Plant DNA from Fresh Tissue. Focus 12: 13–15.
  • Emebiri L, Ogbonnaya F, Moody D, 2008. Population Structure in Wild Emmer (Triticum dicoccoides) Based on EST-SSR Markers.11th International Wheat Genetics Symposium, ISBN: 9781920899141.
  • Fahima T, Röder MS, Wendelhake K, Kirzhmer V, Nevo E, 2002. Microsatellite Polymorphism in Natural Populations of Wild Emmer Wheat, Triticum dicoccoides, Israel. Theoretical and Applied Genetics, 104: 17-29.
  • İlhan D, Li X, Brummer C, Şakiroğlu M, 2016. Genetic Diversity and Population Structure of Tetraploid Accessions of the Medicago sativa-falcata Complex. Crop Science, 56: 1146-1156.
  • Kara K, Rached-Kanouni M, Mnasri S, Khammar H, Ben Naceur MB, 2020. Genetic Variability Assessment in Bread Wheat (Triticum aestivum) Grown in Algeria Using Microsatellites SSR Markers. Biodiversitas, 21: 2638-2644.
  • Kaymaz A, İzbırak A, 2010. Molecular Characterization of Genetic Diversity Among T. dicoccoides and T. dicoccon Populations by RAPD-PCR Technique. Hacettepe Journal of Biology and Chemistry, 3814,333-344.
  • Konvalina P, Capouchova I, Stehno Z, 2012b. Agronomically Important Traits of Emmer. Plant Soil Environment, 58(8): 341-346.
  • Konvalina P, Capouchova I, Stehno Z, Moudry J, 2012a. Differences in Yield Parameters of Emmer in Comparison with Old and New Varieties of Bread Wheat. African Journal of Agricultural Research, 7(6), 986-992.
  • Kumar S, Kumar V, Kumari P, Singh AK, Singh R, 2016. DNA Fingerprinting and Genetic Diversity Studies in Wheat Genotypes Using SSR Markers. Journal of Environmental Biology, 37, 319-326.
  • Li Y, Fahima T, Korol AB, Peng J, Röder MS, Kirzhner V, Beiles A, Nevo E, 2000. Microsatellite Diversity Correlated with Ecological-Edaphic and Genetic Factors in Three Microsites of Wild Emmer in North Israel. Molecular Biology and Evolution, 17 (6), 851-862.
  • Liu J, Muse S, 2005. Power Marker: An Integrated Analysis Environment for Genetic Marker Analysis. Raleigh, NC: North Carolina State University, Bioindormatics Research Center, 21: 2128-2129.
  • Li-xin W, Jun Q, Li-fang C, Li-hua L, Hong-bo L, Bin-shuang P, Chang-ping Z, 2015. Assessment of Wheat Variety Distinctness Using SSR Markers. Journal of Integrative Agriculture, 14(10): 1923-1935.
  • Mondini L, Grausgruber H, Pagnotta MA, 2014. Evaluaiton of European Emmer Germplasm for Agro-Morphological, Grain Quality Traits and Molecular Traits. Genetic Resources and Crop Evolution, 61: 69-87.
  • Mourad Amira MI, Belamkar V, Baenziger PS, 2020. Molecular Genetic Analysis of Spring Wheat Core Collection Using Genetic Diversity, Population Structure, and Linkage Disequilibrium. BMC Genomics, 21:434.
  • Nei M, Tajima F, Tateno Y, 1983. Accuracy of Estimated Phylogenetic Trees from Molecular Data. Journal of Molecular Evolution, 19:153-170.
  • Özkan H, Willcox G, Graner A, Salamini F, Kilian B, 2010. Geographic Distribution and Domestication of Wild Emmer Wheat (Triticum dicoccoides). Genetic Resources and Crop Evoluation, 58:11-53.
  • Pagnotta MA, Mondini L, Codianni P, Fares C, 2009. Agronomical, Quality and Molecular Characterization of Twenty Italian Emmer (Triticum dicoccon) Accessions. Genet Resour Crop Evol, 56: 299-310.
  • Peakall R, Smouse PE, 2001. GenAlEx V6: Genetic Analysis in Excel Population Genetic Software for Teaching and Research. Australian National University, Canberra. http://wwwanueduau/BoZo/ GenAlE.
  • Rafeipour M, Mirzaghaderi G, Shaaf S, Badakhshan H, 2016. SSR Assessment of the Genetic Diversity of Emmer with Emphasis on Iranian Landraces (Triticum dicoccon Schrank). Genetic Resources and Crop Evolution, 63:595-600.
  • Raj RS, Yama SV, Viral KMB, Joshi MN, Shradha NT, Bagatharia SB, 2017. Ascertaining Narrow Genetic Base in Commercial Accessions of Wheat Commonly Grown in Gujarat via Molecular Markers. Electronic Journal of Plant Breeding, 8(2), 558-565.
  • Ren J, Chen L, Sun D, You FM, Wang J, Peng Y, Nevo E, Beiles A, Sun D, Luo M-C, Peng J, 2013. SNP-Revealed Genetic Diversity in Wild Emmer Correlates with Ecological Factors. BMC Evolutionary Biology, 13(1): 169.
  • Robbana C, Kehel Z, Naceur MB, Sansaloni C, Bassi F, Amri A, 2019. Genome-Wide Genetic Diversity and Population Structure of Tunisian Durum Wheat Landraces Based on DArTseq Technology. International Journal of Molecular Sciences, 20(6).
  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW, 1998. A Microsatellite Map of Wheat. Genetics, 149: 2007-2023.
  • Salehi M, Arzani A, Talebi M, Rokhzadi A, 2018. Genetic Diversity of Wheat Wild Relatives Using SSR Markers. Genetika, 50, 131-141.
  • Salem KFM, El-Zanaty AM, Esmail RM, 2008. Assessing Wheat (Triticum aestivum L.) Genetic Diversity Using Morphological Characters and Microsatallite Markers. Word Journal of Agricultural Sciences, 4(5): 538-544.
  • Salunkhe T, Tamhankar S, Tetali S, Zaharieva M, Bonnett D, Trethowan R, Misra S, 2013. Molecular Genetic Diversity Analysis in Emmer (Triticum dicoccon Schrank) from India. Genetic Resource and Crop Evolution, 60:165-174.
  • Shizuka T, Mori N, Ozkan H, Ohta S, 2015. Chloroplast DNA Haplotype Variation within Two Natural Populations of Wild Emmer Wheat (Triticum turgidum ssp. dicoccoides) in Southern Turkey. Biotechnology & Biotechnological Equipment, 29 (3): 423-430.
  • Tahır R, Abdul N, 2010. Germination Characteristics and Molecular Characterizations of Some Wheat Varieties in Sulaimanyah by SSR Marker. Turk Journal Biology, 34:109-117.
  • Tekin M, Cengiz MF, Abbasov M, Aksoy A, 2018. Comparison of Some Mineral Nutrients and Vitamins in Advanced Hulled Wheat Lines. Research Article, Cereal Chemistry; 95(3): 436-444.
  • Teklu Y, Hammer K, Huang XQ, Röder MS, 2006. Analysis of Microsatellite Diversity in Ethiopian Tetraploid Wheat Landraces. Genetic Resources and Crop Evolution, 53:1115-1126.
  • Teklu Y, Hammer K, Röder MS, 2007. Simple Sequence Marker Polymorphism in Emmer (Triticum dicoccon Schrank): Analysis of Genetic Diversity and Differentiation. Genetic Resource and Crop Evolution, 54:543-554.
  • Zarei Abbasabad E, Mohammadi SA, Moghaddam M, Jalal Kamali MR, 2016. Analysis of Genetic Diversity, Population Structure and Linkage Disequilibrium in Iranian Wheat Landraces Using SSR Markers. Plant Genetic Resources 1:1-8.
  • Zatybekov A, Anuarbek S, Abugalieva S, Turuspekov Y, 2020. Phenotypic and Genetic Variability of Tetraploid Wheat Collection Grown in Kazakhstan. Vavilovskii Zhurnal Genet Selektsii, 24(6): 605-612.

Genetic Diversity of Turkish Cultivated Emmer (Triticum dicoccum Schrank) Revealed by Microsatellite Markers

Year 2021, , 3119 - 3127, 15.12.2021
https://doi.org/10.21597/jist.911056

Abstract

The aim of this study is to evaluate the genetic diversity of twelve populations for cultivated emmer (Triticum dicoccum Schrank ex Schübl) and bread wheat (Triticum aestivum L.) species, which are important wheat gene resources and grown in different villages in Kars province (Turkey), using eleven microsatellite markers. SSR primers produced a total of 41 alleles and the average polymorphism percentage was 86.2%. The average number of alleles obtained from primers was 3.72. Polymorphic Information Content (PIC) values varied between 0.14 and 0.37 with the means of 0.26 value. The primers of Xgwm-46 (0.287), Xgwm-154 (0.304) and Xgwmn-361 (0.325) were identified as the most effective primers in understanding the genetic diversity of emmer genotypes. Local emmer wheat had a little higher allelic richness and gene diversity than cultivated wheat. Due to geographic and climatic variations, genetic differentiation was detected in these wheat populations.

Project Number

2018-FM-16

References

  • Abbasov M, Akparov Z, Gross T, Babayeva S, Izzatullayeva V, Hajiyev E, Rustamov K, Gross P, Tekin M, Akar T, Chao S, Brueggeman R, 2018. Genetic Relationship of Diploid Wheat (Triticum spp.) Species Assessed by SSR Markers. Genetic Resources and Crop Evolution; 65(5):1441-1453.
  • Akfirat FS, Uncuoglu AA, 2013. Genetic Diversity of Winter Wheat (Triticum aestivum L.) Revealed by SSR Markers. Biochemical Genetics, 51:223-229. DOI: 10.1007/s10528-012-9557-6.
  • Arystanbekkyzy M, Nadeem MA, Aktaş H, Yeken MZ, Zencirci N, Nawaz MA, Ali F, Haider MS, Tunc K, Chung G, Baloch FS, 2018. Phylogenetic and Taxonomic Relationship of Turkish Wild and Cultivated Emmer (Triticum turgidum ssp. dicoccoides) Revealed by IPBS-Retrotransposons Markers. International Journal of Agriculture and Biology, 21(1): 155-163.
  • Belete YS, Shimelis H, Laing MK, Mathew I, 2020. Genetic Diversity and Population Structure of Bread Wheat Genotypes Determined via Phenotypic and SSR Marker Analyses under Drought-Stress Conditions. Journal of Crop Improvement, doi: 10.1080/15427528.2020.1818342.
  • Chen X, Min D, Yasir TA, Hu Y-G, 2012. Genetic Diversity, Population Structure and Linkage Disequilibrium in Elite Chinese Winter Wheat Investigated with SSR Markers. PLoS One 7(9): e44510.
  • Demirel F, 2020. Genetic Diversity of Emmer Wheats Using IPBS-Retrotranspozon Markers. European Journal of Science and Technology, 20, 640-646.
  • Dong P, Wei Y-M, Chen G-Y, Li W, Wang J-R, Nevo E, Zheng Y-L, 2009. EST-SSR Diversity Correlated with Ecological and Genetic Factors of Wild Emmer in Israel. Hereditas 146: 1-10.
  • Doyle JJ, Doyle JL, 1990. Isolation of Plant DNA from Fresh Tissue. Focus 12: 13–15.
  • Emebiri L, Ogbonnaya F, Moody D, 2008. Population Structure in Wild Emmer (Triticum dicoccoides) Based on EST-SSR Markers.11th International Wheat Genetics Symposium, ISBN: 9781920899141.
  • Fahima T, Röder MS, Wendelhake K, Kirzhmer V, Nevo E, 2002. Microsatellite Polymorphism in Natural Populations of Wild Emmer Wheat, Triticum dicoccoides, Israel. Theoretical and Applied Genetics, 104: 17-29.
  • İlhan D, Li X, Brummer C, Şakiroğlu M, 2016. Genetic Diversity and Population Structure of Tetraploid Accessions of the Medicago sativa-falcata Complex. Crop Science, 56: 1146-1156.
  • Kara K, Rached-Kanouni M, Mnasri S, Khammar H, Ben Naceur MB, 2020. Genetic Variability Assessment in Bread Wheat (Triticum aestivum) Grown in Algeria Using Microsatellites SSR Markers. Biodiversitas, 21: 2638-2644.
  • Kaymaz A, İzbırak A, 2010. Molecular Characterization of Genetic Diversity Among T. dicoccoides and T. dicoccon Populations by RAPD-PCR Technique. Hacettepe Journal of Biology and Chemistry, 3814,333-344.
  • Konvalina P, Capouchova I, Stehno Z, 2012b. Agronomically Important Traits of Emmer. Plant Soil Environment, 58(8): 341-346.
  • Konvalina P, Capouchova I, Stehno Z, Moudry J, 2012a. Differences in Yield Parameters of Emmer in Comparison with Old and New Varieties of Bread Wheat. African Journal of Agricultural Research, 7(6), 986-992.
  • Kumar S, Kumar V, Kumari P, Singh AK, Singh R, 2016. DNA Fingerprinting and Genetic Diversity Studies in Wheat Genotypes Using SSR Markers. Journal of Environmental Biology, 37, 319-326.
  • Li Y, Fahima T, Korol AB, Peng J, Röder MS, Kirzhner V, Beiles A, Nevo E, 2000. Microsatellite Diversity Correlated with Ecological-Edaphic and Genetic Factors in Three Microsites of Wild Emmer in North Israel. Molecular Biology and Evolution, 17 (6), 851-862.
  • Liu J, Muse S, 2005. Power Marker: An Integrated Analysis Environment for Genetic Marker Analysis. Raleigh, NC: North Carolina State University, Bioindormatics Research Center, 21: 2128-2129.
  • Li-xin W, Jun Q, Li-fang C, Li-hua L, Hong-bo L, Bin-shuang P, Chang-ping Z, 2015. Assessment of Wheat Variety Distinctness Using SSR Markers. Journal of Integrative Agriculture, 14(10): 1923-1935.
  • Mondini L, Grausgruber H, Pagnotta MA, 2014. Evaluaiton of European Emmer Germplasm for Agro-Morphological, Grain Quality Traits and Molecular Traits. Genetic Resources and Crop Evolution, 61: 69-87.
  • Mourad Amira MI, Belamkar V, Baenziger PS, 2020. Molecular Genetic Analysis of Spring Wheat Core Collection Using Genetic Diversity, Population Structure, and Linkage Disequilibrium. BMC Genomics, 21:434.
  • Nei M, Tajima F, Tateno Y, 1983. Accuracy of Estimated Phylogenetic Trees from Molecular Data. Journal of Molecular Evolution, 19:153-170.
  • Özkan H, Willcox G, Graner A, Salamini F, Kilian B, 2010. Geographic Distribution and Domestication of Wild Emmer Wheat (Triticum dicoccoides). Genetic Resources and Crop Evoluation, 58:11-53.
  • Pagnotta MA, Mondini L, Codianni P, Fares C, 2009. Agronomical, Quality and Molecular Characterization of Twenty Italian Emmer (Triticum dicoccon) Accessions. Genet Resour Crop Evol, 56: 299-310.
  • Peakall R, Smouse PE, 2001. GenAlEx V6: Genetic Analysis in Excel Population Genetic Software for Teaching and Research. Australian National University, Canberra. http://wwwanueduau/BoZo/ GenAlE.
  • Rafeipour M, Mirzaghaderi G, Shaaf S, Badakhshan H, 2016. SSR Assessment of the Genetic Diversity of Emmer with Emphasis on Iranian Landraces (Triticum dicoccon Schrank). Genetic Resources and Crop Evolution, 63:595-600.
  • Raj RS, Yama SV, Viral KMB, Joshi MN, Shradha NT, Bagatharia SB, 2017. Ascertaining Narrow Genetic Base in Commercial Accessions of Wheat Commonly Grown in Gujarat via Molecular Markers. Electronic Journal of Plant Breeding, 8(2), 558-565.
  • Ren J, Chen L, Sun D, You FM, Wang J, Peng Y, Nevo E, Beiles A, Sun D, Luo M-C, Peng J, 2013. SNP-Revealed Genetic Diversity in Wild Emmer Correlates with Ecological Factors. BMC Evolutionary Biology, 13(1): 169.
  • Robbana C, Kehel Z, Naceur MB, Sansaloni C, Bassi F, Amri A, 2019. Genome-Wide Genetic Diversity and Population Structure of Tunisian Durum Wheat Landraces Based on DArTseq Technology. International Journal of Molecular Sciences, 20(6).
  • Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW, 1998. A Microsatellite Map of Wheat. Genetics, 149: 2007-2023.
  • Salehi M, Arzani A, Talebi M, Rokhzadi A, 2018. Genetic Diversity of Wheat Wild Relatives Using SSR Markers. Genetika, 50, 131-141.
  • Salem KFM, El-Zanaty AM, Esmail RM, 2008. Assessing Wheat (Triticum aestivum L.) Genetic Diversity Using Morphological Characters and Microsatallite Markers. Word Journal of Agricultural Sciences, 4(5): 538-544.
  • Salunkhe T, Tamhankar S, Tetali S, Zaharieva M, Bonnett D, Trethowan R, Misra S, 2013. Molecular Genetic Diversity Analysis in Emmer (Triticum dicoccon Schrank) from India. Genetic Resource and Crop Evolution, 60:165-174.
  • Shizuka T, Mori N, Ozkan H, Ohta S, 2015. Chloroplast DNA Haplotype Variation within Two Natural Populations of Wild Emmer Wheat (Triticum turgidum ssp. dicoccoides) in Southern Turkey. Biotechnology & Biotechnological Equipment, 29 (3): 423-430.
  • Tahır R, Abdul N, 2010. Germination Characteristics and Molecular Characterizations of Some Wheat Varieties in Sulaimanyah by SSR Marker. Turk Journal Biology, 34:109-117.
  • Tekin M, Cengiz MF, Abbasov M, Aksoy A, 2018. Comparison of Some Mineral Nutrients and Vitamins in Advanced Hulled Wheat Lines. Research Article, Cereal Chemistry; 95(3): 436-444.
  • Teklu Y, Hammer K, Huang XQ, Röder MS, 2006. Analysis of Microsatellite Diversity in Ethiopian Tetraploid Wheat Landraces. Genetic Resources and Crop Evolution, 53:1115-1126.
  • Teklu Y, Hammer K, Röder MS, 2007. Simple Sequence Marker Polymorphism in Emmer (Triticum dicoccon Schrank): Analysis of Genetic Diversity and Differentiation. Genetic Resource and Crop Evolution, 54:543-554.
  • Zarei Abbasabad E, Mohammadi SA, Moghaddam M, Jalal Kamali MR, 2016. Analysis of Genetic Diversity, Population Structure and Linkage Disequilibrium in Iranian Wheat Landraces Using SSR Markers. Plant Genetic Resources 1:1-8.
  • Zatybekov A, Anuarbek S, Abugalieva S, Turuspekov Y, 2020. Phenotypic and Genetic Variability of Tetraploid Wheat Collection Grown in Kazakhstan. Vavilovskii Zhurnal Genet Selektsii, 24(6): 605-612.
There are 40 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Moleküler Biyoloji ve Genetik / Moleculer Biology and Genetic
Authors

Hatice Demir 0000-0003-4790-7729

Doğan İlhan 0000-0003-2805-1638

Project Number 2018-FM-16
Publication Date December 15, 2021
Submission Date April 7, 2021
Acceptance Date June 11, 2021
Published in Issue Year 2021

Cite

APA Demir, H., & İlhan, D. (2021). Genetic Diversity of Turkish Cultivated Emmer (Triticum dicoccum Schrank) Revealed by Microsatellite Markers. Journal of the Institute of Science and Technology, 11(4), 3119-3127. https://doi.org/10.21597/jist.911056
AMA Demir H, İlhan D. Genetic Diversity of Turkish Cultivated Emmer (Triticum dicoccum Schrank) Revealed by Microsatellite Markers. Iğdır Üniv. Fen Bil Enst. Der. December 2021;11(4):3119-3127. doi:10.21597/jist.911056
Chicago Demir, Hatice, and Doğan İlhan. “Genetic Diversity of Turkish Cultivated Emmer (Triticum Dicoccum Schrank) Revealed by Microsatellite Markers”. Journal of the Institute of Science and Technology 11, no. 4 (December 2021): 3119-27. https://doi.org/10.21597/jist.911056.
EndNote Demir H, İlhan D (December 1, 2021) Genetic Diversity of Turkish Cultivated Emmer (Triticum dicoccum Schrank) Revealed by Microsatellite Markers. Journal of the Institute of Science and Technology 11 4 3119–3127.
IEEE H. Demir and D. İlhan, “Genetic Diversity of Turkish Cultivated Emmer (Triticum dicoccum Schrank) Revealed by Microsatellite Markers”, Iğdır Üniv. Fen Bil Enst. Der., vol. 11, no. 4, pp. 3119–3127, 2021, doi: 10.21597/jist.911056.
ISNAD Demir, Hatice - İlhan, Doğan. “Genetic Diversity of Turkish Cultivated Emmer (Triticum Dicoccum Schrank) Revealed by Microsatellite Markers”. Journal of the Institute of Science and Technology 11/4 (December 2021), 3119-3127. https://doi.org/10.21597/jist.911056.
JAMA Demir H, İlhan D. Genetic Diversity of Turkish Cultivated Emmer (Triticum dicoccum Schrank) Revealed by Microsatellite Markers. Iğdır Üniv. Fen Bil Enst. Der. 2021;11:3119–3127.
MLA Demir, Hatice and Doğan İlhan. “Genetic Diversity of Turkish Cultivated Emmer (Triticum Dicoccum Schrank) Revealed by Microsatellite Markers”. Journal of the Institute of Science and Technology, vol. 11, no. 4, 2021, pp. 3119-27, doi:10.21597/jist.911056.
Vancouver Demir H, İlhan D. Genetic Diversity of Turkish Cultivated Emmer (Triticum dicoccum Schrank) Revealed by Microsatellite Markers. Iğdır Üniv. Fen Bil Enst. Der. 2021;11(4):3119-27.