The Effect of Trolox on Oxidative Stress Index and Nitric Oxide Levels

Abstract

Free radicals, which are formed as a consequence of endogenic and exogenic factors in cells, that cause oxidative stress in living organisms can be neutralized through catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), A, E, C vitamins, glutathione, ubiquinone, and flavonoids. The aim of this study is to investigate the effect of trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a type of vitamin E, on rabbits regarding the total oxidant and antioxidant capacity (TOC, TAC) levels together with the NO levels. In this study, 0.5 ml physiological saline and 1 µmol kg-1 trolox were given respectively to control and experiment rabbits via intraperitoneal (i.p.) route, Plasmas of blood samples, which were obtained in the 1st, 3rd, and 6th hours following injection, were separated and stored at -20 oC until to be analyzed. Plasma TOC, TAC and NO levels were determined spectrophotometrically. When the TOC, TAC, NO levels and OSI values of rabbits that were given trolox were compared to those of the control group, statistically, it was observed that the NO levels were high (p< 0,01) in the 1st, 3rd, and 6th hours; however, there was no alteration in their TAC, TOC levels and OSI values. As a result, it was concluded that trolox given as a single dose to healthy rabbits did not affect TAC TOC levels and OSI value, but the increasing levels of NO might be due to trolox's increasing activity of eNOS.

Keywords

• Antioxidant capacity
• Oxidant capacity
• Nitric oxide
• Trolox

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