Sulfonate Group Containing Schiff Base Ligand and Their Transition Metal Complexes: Synthesis, Structural Characterization and Antimicrobial Activity Studies

Abstract

In this study, H4L Schiff base ligand which can form stable complexes with 4,4′-Diamino-2,2′-stilbenedisulfonic acid compound having primary diamine and disulfonic acid group in its molecular structure was synthesized. Cu(II), Zn(II) and Cd(II) complex compounds of the H4 Lligand were synthesized. Molecular structures of the compounds obtained were characterized using analytical and spectroscopic methods. Gram positive(5), gram negative(6), probiotic bacteria(2) antimicrobial and antifungal activities (MIC, MBC) of all compounds were studied. MIC values of the compounds on indicator bacteria at concentrations of 500, 250, 62.5, 31.2, 15.63 and 7.81 μg/mL, 250 mg/mL for H4L, 31.5 mg/mL for [Cu2H2L(CH3COO)2(H2O)2], 62.5 mg/mL for [Cd2H2LCl2(H2O2] and 125 mg/mL for [Zn2H2L(CH3COO)2(H2O)2], the complexes were determined to be more effective than the ligand. Bactericidal effects were determined in the MBC test at concentrations of 500-250 mg/mL. In the agar well diffusion test, the compounds were tested only at 500 mg/ml and different inhibition diameters were determined.

Keywords

• Complex compounds
• antimicrobial activity
• gram positive

Sülfonat Grubu İçeren Schiff Bazı Ligandı ve Geçiş Metal Kompleksleri: Sentezi, Yapısal Karakterizasyonları ve Antimikrobiyal Aktivitelerinin İncelenmesi

Abstract

Bu çalışmada, molekül yapısında primer diamin ve disülfonik asit grubu bulunduran 4,4′-Diamino-2,2′-stilbendisülfonik asit bileşiği ile 2,4-dihidroksi benzaldehit kullanılarak kararlı kompleksler oluşturabilen H4L Schiff bazı ligandı sentezlenmiştir. H4L ligandının Cu(II), Zn(II) ve Cd(II) kompleks bileşikleri sentezlenmiştir. Elde edilen bileşiklerin molekül yapıları analitik ve spektroskopik yöntemler kullanılarak karakterize edilmiştir. Tüm bileşiklerin gram pozitif (5), gram negatif (6) ve probiyotik bakterileri (2) kullanılarak antimikrobiyal aktiviteleri ve antifungal aktivite (MIC, MBC) çalışmaları yapılmıştır. Bileşiklerin 500, 250, 62.5, 31.2, 15.63 ve 7.81 μg/mL konsantrasyonlarda indikatör bakterileri üzerine MIC değerleri, H4L için 250 mg/mL, [Cu2H2L(CH3COO)2(H2O)2] için 31.5 mg/mL, [Cd2H2LCl2(H2O)2] için 62.5 mg/mL, [Zn2H2L(CH3COO)2(H2O)2] için 125 mg/mL olarak komplekslerin ligantdan daha etkili olduğu belirlenmiştir. MBC testinde 500-250 mg/mL konsantrasyonlarında bakterisid etki belirlenmiştir. Agar kuyu difüzyon testinde ise bileşikler sadece 500 mg/ml’de test edilmiş ve farklı inhibisyon çapları belirlenmiştir.

Keywords

• Kompleks bileşikler
• antimikrobiyal aktivite
• gram pozitif

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