Investigation of Potential Usability of Native Bacillus thuringiensis Isolates in Biological Control of Disease Vector, Culex pipiens (Diptera:Culicidae)

Year 2017, Volume: 7 Issue: 1, 47 - 53, 31.03.2017

Uğur Azizoğlu

Abstract

Culex pipiens (Diptera: Culicidae) is primary vector of West Nile virus. Products of entomopathogen
bacterium Bacillus thuringiensis (Bt) has effectively been used for decades for decreasing the chemical input into
the environment and C. pipiens mediated diseases. In the current study, biocontrol potential of 14 different native Bt
isolates was investigated on C. pipiens larvae. Last instar larvae of C. pipens were subjected to spore-crystal protein
mixture (500 µg ml-1) of 14 different Bt isolates carrying dipteran active cry genes. Of all the tested isolates, the
products of Bt SY50.4 caused 80% mortality on the larvae. Although Bt SY56.3 showed lower insecticidal effect
than that of Bt SY50.4, the activity of this isolate was higher than control. The other tested isolates didn’t show
signifcant mortality on larvae of the C. pipiens. Bt SY50.4 was determined as promising an isolate based on the
effectiveness on the C. pipiens larvae. Pupation and adult emergence periods of remaining larvae after treatment
were not signifcantly different when compared with the control

Keywords

Bacillus thuringiensis, biological control, Culex pipiens, life cycle

Hastalık Taşıyıcısı Culex pipiens (Diptera:Culicidae)’ in Biyolojik Mücadelesinde Yerel Bacillus thuringiensis İzolatlarının Kullanılabilme Potansiyelinin Araştırılması

Abstract

Culex pipiens (Diptera: Culicidae) Batı Nil Virüs’ünün başlıca vektörüdür. Kimyasal pestisit kullanımının
ve C. pipiens kaynaklı hastalıkların azaltılması için entomopatojen bakteri Bacillus thuringiensis (Bt) ürünleri
yıllardır etkili bir şekilde kullanılmaktadır. Bu çalışmada, 14 farklı yerel Bt izolatının C. pipiens larvaları üzerindeki
biyolojik mücadele potansiyeli araştırılmıştır. Dipteran spesifk cry gen taşıyan 14 farklı Bt izolatının spor-kristal
protein karşımı (500 µg ml-1) C. pipens’in son dönem larvalarına uygulanmıştır. Uygulanan izolatlar arasında Bt
SY50.4’ün spor-kristal protein karşımı % 80 larval ölüme sebep olmuştur. Bt SY56.3 izolatı, Bt SY50.4 kadar etkili
olmasa da kontrolden daha fazla ölüm oranına sebep olmuştur. Diğer izolatlar C. pipens larvaları üzerinde önemli bir
etki göstermemiştir. Bt SY50.4 izolatının C. pipiens üzerindeki etkinliği göz önüne alındığında biyolojik mücadele
açısından gelecek vaat ettiği belirlenmiştir. Uygulama sonrası yaşayan larvaların pupalaşma ve erginleşme süreçleri
kontrol grubu ile karşılaştırıldığında aralarında önemli bir fark olmadığı belirlenmiştir

Keywords

Bacillus thuringiensis, biyolojik mücadele, Culex pipiens, hayat döngüsü

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