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Investigations of in vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant

Year 2017, Volume: 7 Issue: 2, 141 - 148, 30.06.2017

Abstract

In our study, some in vitro biological activities of hexane (HEG), ethylacetate (EtOAc) and methanol
(MeOH) extracts of
Acanthus dioscoridis L. var. dioscoridis L. aerial parts have been investigated in details. Plant
extracts were tested in view of its
in vitro antioxidant activities as total phenolic and total flavonoid contents, DPPH
free radical-scavenging, metal chelating and reducing power activity. Total phenolic content of MeOH extract
has been found as 71.18 μg GAE/mg. The highest amount of flavonoids has been detected in EtOAc extract. The
highest DPPH radical-scavenging activity and reducing power activity has been determined in MeOH extract.
HEG extracts of
Acanthus dioscoridis has showed clearly higher metal chelating activity when compared to other
extracts.
SPUFA percentage was meaningfully higher than SMUFA and SSFA. By considering the high PUFA
content and strong antioxidant activities it could be concluded that
A. dioscoridis could be recommended to possible
use in medical science, cosmetic and food industry.



References

  • Amiguet VT, Kramp KL, Mao J, McRae C, Goulah A, Kimpe LE, et al., 2012. Supercritical carbondioxide extraction of polyunsaturated fatty acids from Northern shrimp (P andalus borealis Kreyer) processing by-products. Food Chemistry, 130(4): 853-858.
  • Anderson RA, 1995. Chromium and parenteral nutrition. Nutrition, 11(1): 83-86.
  • Anderson RA, 1993. Recent advances in the clinical and biochemical effects of chromium deficiency. Progress in Clinical and Biological Research, 380: 221-234.
  • da Silva JA, Naeem M, Idrees M, 2012. Beneficial and toxic effects of nickel in relation to medicinal and aromatic plants. Medicinal and Aromatic Plant Science and Biotechnology, 6(1): 94–104.
  • Davis P.H. Flora of Turkey and East Eagean Islands. 1993; Edinburgh UniversitiyPress, Edinburgh.
  • Dorman, HJD, Hiltunen R, 2004. Fe(III) reductive and free radical-scavenging properties of summer savory (Saturejahortensis L.) extract and subfractions. Food Chemistry, 88: 193-199.
  • Elham A, Mohammed MR, El-Hawary SS, Magda M.F, Rabab M, James J, et al., 2014. Potent insecticidal secondary metabolites from the medicinal plant Acanthus montanus. Records of Natural Products, 6(3): 301-305.
  • Esetlili BÇ, Pekcan T, Çobanoğlu Ö, Aydoğdu E, Turan S, Anaç D, 2014. Essential plant nutrients and heavy metals concentrations of some medicinal and aromatic plants. Journal of Agricultural Science, 20: 239-247.
  • FAO/WHO/IAEA, 1996. Trace elements in human nutrition and health. World health organization, Geneva, Switzerland.
  • Flora G, Gupta D, Tiwari A, 2012. Toxicity of lead: a review with recent updates. Interdisciplinary Toxicology, 5: 47–58.
  • FSA, 2003. Safe upper levels for vitamins and minerals. Expert group on vitamins and minerals. Food Standards Agency. ISBN 1-904026-11-7.
  • Garces R, Mancha M, 1993. One step lipid extraction and fatty acid methylesters preparation from tree plant tissues. Analytical Biochemistry, 211: 139–143.
  • Gharibi S, Tabatabaei BES, Saeidi G, 2015. Comparison of essential oil composition, flavonoid content and antioxidant activity in eight Achillea species. Journal of Essential Oil Bearing Plants, 18: 1382–1394.
  • Hai F, Xu-li T, Guo-qiang L, 2009. Sterols from the mangrove plant Acanthus ilicifolius. Chinese Journal of Marine Drugs, 28: 23-28.
  • Huang MY, Zhong LJ, Quan-YuLiu FW, Zhang YH. Chemical Constituents from the Roots of Acanthus ilicifolius Biochemical Systematics and Ecology, 2014; 55: 145-147.
  • Keskin C, Kişin E, Yavuz M, 2014.Trace metal determination in the medicinal plant Hyoscyamus (Solanaceae) by inductively coupled plasma optical emission spectrometry. Atomic Spectroscopy, 35(5): 193-199.
  • Lisa HM, Jordan PH, Dong YH, 2014. Pb Neurotoxicity: Neuropsychological effects of lead toxicity. BioMed Research International, 1-9, doi:10.1155/2014/840547.
  • Moreno MIN, Isla MI, Sampietro AR, Vattuone MA, 2000. Comparison of the free radical-scavenging activity of propolis from several regions of argentina. Journal of Ethnopharmacology, 71: 109–114.
  • Murphy DJ, 1990. Storage lipid bodies in plants and other organisms. Progress in Lipid Research, 29(4): 299-324.
  • Oyaizu M, 1988. Antioxidative activities of browning products of glucosamine fractioned by organic solvent and thin-layer chromatography, Nippon Shokuhin Kogyo Gakkaishi, 35: 771-775.
  • Pietta PG, 2000. Flavonoids as antioxidants. Journal of Natural Products, 63: 1035-1042.
  • Rahimmalek M, Bahreininejad B, Khorrami M, Tabatabaei BES, 2009. Genetic variability and geographic differentiation in Thymus daenensis subsp. daenensis, an endangered medicinal plant, as revealed by inter simple sequence repeat (ISSR) markers. Biochemical Genetics, 47: 831–842.
  • Saura-Calixto F, Goni I, 2006. Antioxidant capacity of the Spanish Mediterranean diet. Food Chemistry, 94: 442-447.
  • Shimada K, Fujikawa K, Yahara K, Nakamura T, 1992. Antioxidative properties of xanthin and autooxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry, 40: 945–948.
  • Singh D, Aeri V, 2013. Phytochemical and pharmacological potential of Acanthus ilicifolius. Journal of Pharmacy and Bioallied Sciences, 5: 17-20.
  • Singh R, Singh S, Kumar S, Arora S, 2007. Studies on antioxidant potential of methanol extract/fractions of Acacia auriculiformis A. Cunn. Food Chemistry, 103: 505-511.
  • Slinkard K, Singleton VL, 1997. Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1): 49-55.
  • Stanković MS, 2011. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac Journal of Scence, 33: 63-72.
  • Stern BR, 2010. Essentiality and toxicity in copper health risk assessment: overview, update and regulatory considerations. Journal of Toxicology and Environmental Health, Part A, 73(2): 114–127.
  • Tohidi B, Rahimmalek M, Arzani A, 2017. Essential oil composition, total phenolic, flavonoid contents, and antioxidant activity of Thymus species collected from different regions of Iran. Food Chemistry, 220: 153–161
  • WHO/FAO/IAEA, 1996. World health organization. Trace elements in human nutrition and health, Switzerland: Geneva.

Tıbbi Bir Bitki Olarak Acanthus dioscoridis L. var. dioscoridis’ in in vitro Antioksidan Aktiviteleri, Elementel Bileşimi ve Lipit Bileşenlerinin Araştırılması

Year 2017, Volume: 7 Issue: 2, 141 - 148, 30.06.2017

Abstract

Bu çalışmamızda Acanthus dioscoridis L. var. dioscoridis L.’ in toprak üstü kısımlarının hekzan (HEG),
etilasetat (EtOAc) ve metanol özütlerinin bazı
in vitro biyolojik aktiviteleri detaylı olarak incelenmiştir. Bitki
özütlerinin
in vitro antioksidan aktiviteleri total fenolik ve flavonoid içerikleri, DPPH serbest radikal söndürücü,
metal şelatlama ve indirgeme gücü aktiviteleri bakımından test edildi. MeOH özütünün toplam fenolik madde
içeriği 71.18 μg GAE/mg olarak tespit edilmiştir. En yüksek flavonoid miktarı EtOAc özütünde tespit edilmiştir. En
yüksek DPPH radikali söndürme aktivitesi ve indirgeme gücü aktivitesi MeOH özütünde belirlenmiştir.
Acanthus
dioscoridis
L. var. dioscoridis L.’ nin HEG özütü diğer özütler ile karşılaştırıldığında açık bir şekilde daha yüksek
metal şelatlama aktivitesi göstermiştir.
SPUFA yüzdesi anlamlı olarak SMUFA ve SSFA’ dan daha yüksekti. Yüksek
PUFA içeriği ve güçlü antioksidan aktiviteleri göz önüne alındığında
A. dioscoridis’ in tıbbi bilimlerde, kozmetik
ve gıda endüstrisinde olası kullanımı önerilebilir



References

  • Amiguet VT, Kramp KL, Mao J, McRae C, Goulah A, Kimpe LE, et al., 2012. Supercritical carbondioxide extraction of polyunsaturated fatty acids from Northern shrimp (P andalus borealis Kreyer) processing by-products. Food Chemistry, 130(4): 853-858.
  • Anderson RA, 1995. Chromium and parenteral nutrition. Nutrition, 11(1): 83-86.
  • Anderson RA, 1993. Recent advances in the clinical and biochemical effects of chromium deficiency. Progress in Clinical and Biological Research, 380: 221-234.
  • da Silva JA, Naeem M, Idrees M, 2012. Beneficial and toxic effects of nickel in relation to medicinal and aromatic plants. Medicinal and Aromatic Plant Science and Biotechnology, 6(1): 94–104.
  • Davis P.H. Flora of Turkey and East Eagean Islands. 1993; Edinburgh UniversitiyPress, Edinburgh.
  • Dorman, HJD, Hiltunen R, 2004. Fe(III) reductive and free radical-scavenging properties of summer savory (Saturejahortensis L.) extract and subfractions. Food Chemistry, 88: 193-199.
  • Elham A, Mohammed MR, El-Hawary SS, Magda M.F, Rabab M, James J, et al., 2014. Potent insecticidal secondary metabolites from the medicinal plant Acanthus montanus. Records of Natural Products, 6(3): 301-305.
  • Esetlili BÇ, Pekcan T, Çobanoğlu Ö, Aydoğdu E, Turan S, Anaç D, 2014. Essential plant nutrients and heavy metals concentrations of some medicinal and aromatic plants. Journal of Agricultural Science, 20: 239-247.
  • FAO/WHO/IAEA, 1996. Trace elements in human nutrition and health. World health organization, Geneva, Switzerland.
  • Flora G, Gupta D, Tiwari A, 2012. Toxicity of lead: a review with recent updates. Interdisciplinary Toxicology, 5: 47–58.
  • FSA, 2003. Safe upper levels for vitamins and minerals. Expert group on vitamins and minerals. Food Standards Agency. ISBN 1-904026-11-7.
  • Garces R, Mancha M, 1993. One step lipid extraction and fatty acid methylesters preparation from tree plant tissues. Analytical Biochemistry, 211: 139–143.
  • Gharibi S, Tabatabaei BES, Saeidi G, 2015. Comparison of essential oil composition, flavonoid content and antioxidant activity in eight Achillea species. Journal of Essential Oil Bearing Plants, 18: 1382–1394.
  • Hai F, Xu-li T, Guo-qiang L, 2009. Sterols from the mangrove plant Acanthus ilicifolius. Chinese Journal of Marine Drugs, 28: 23-28.
  • Huang MY, Zhong LJ, Quan-YuLiu FW, Zhang YH. Chemical Constituents from the Roots of Acanthus ilicifolius Biochemical Systematics and Ecology, 2014; 55: 145-147.
  • Keskin C, Kişin E, Yavuz M, 2014.Trace metal determination in the medicinal plant Hyoscyamus (Solanaceae) by inductively coupled plasma optical emission spectrometry. Atomic Spectroscopy, 35(5): 193-199.
  • Lisa HM, Jordan PH, Dong YH, 2014. Pb Neurotoxicity: Neuropsychological effects of lead toxicity. BioMed Research International, 1-9, doi:10.1155/2014/840547.
  • Moreno MIN, Isla MI, Sampietro AR, Vattuone MA, 2000. Comparison of the free radical-scavenging activity of propolis from several regions of argentina. Journal of Ethnopharmacology, 71: 109–114.
  • Murphy DJ, 1990. Storage lipid bodies in plants and other organisms. Progress in Lipid Research, 29(4): 299-324.
  • Oyaizu M, 1988. Antioxidative activities of browning products of glucosamine fractioned by organic solvent and thin-layer chromatography, Nippon Shokuhin Kogyo Gakkaishi, 35: 771-775.
  • Pietta PG, 2000. Flavonoids as antioxidants. Journal of Natural Products, 63: 1035-1042.
  • Rahimmalek M, Bahreininejad B, Khorrami M, Tabatabaei BES, 2009. Genetic variability and geographic differentiation in Thymus daenensis subsp. daenensis, an endangered medicinal plant, as revealed by inter simple sequence repeat (ISSR) markers. Biochemical Genetics, 47: 831–842.
  • Saura-Calixto F, Goni I, 2006. Antioxidant capacity of the Spanish Mediterranean diet. Food Chemistry, 94: 442-447.
  • Shimada K, Fujikawa K, Yahara K, Nakamura T, 1992. Antioxidative properties of xanthin and autooxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry, 40: 945–948.
  • Singh D, Aeri V, 2013. Phytochemical and pharmacological potential of Acanthus ilicifolius. Journal of Pharmacy and Bioallied Sciences, 5: 17-20.
  • Singh R, Singh S, Kumar S, Arora S, 2007. Studies on antioxidant potential of methanol extract/fractions of Acacia auriculiformis A. Cunn. Food Chemistry, 103: 505-511.
  • Slinkard K, Singleton VL, 1997. Total phenol analysis: Automation and comparison with manual methods. American Journal of Enology and Viticulture, 28(1): 49-55.
  • Stanković MS, 2011. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac Journal of Scence, 33: 63-72.
  • Stern BR, 2010. Essentiality and toxicity in copper health risk assessment: overview, update and regulatory considerations. Journal of Toxicology and Environmental Health, Part A, 73(2): 114–127.
  • Tohidi B, Rahimmalek M, Arzani A, 2017. Essential oil composition, total phenolic, flavonoid contents, and antioxidant activity of Thymus species collected from different regions of Iran. Food Chemistry, 220: 153–161
  • WHO/FAO/IAEA, 1996. World health organization. Trace elements in human nutrition and health, Switzerland: Geneva.
There are 31 citations in total.

Details

Primary Language English
Journal Section Gıda Mühendisliği / Food Engineering
Authors

Cumali Keskin

Publication Date June 30, 2017
Submission Date November 7, 2016
Acceptance Date May 17, 2017
Published in Issue Year 2017 Volume: 7 Issue: 2

Cite

APA Keskin, C. (2017). Investigations of in vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant. Journal of the Institute of Science and Technology, 7(2), 141-148.
AMA Keskin C. Investigations of in vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant. J. Inst. Sci. and Tech. June 2017;7(2):141-148.
Chicago Keskin, Cumali. “Investigations of in Vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus Dioscoridis L. Var. Dioscoridis L. As a Medicinal Plant”. Journal of the Institute of Science and Technology 7, no. 2 (June 2017): 141-48.
EndNote Keskin C (June 1, 2017) Investigations of in vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant. Journal of the Institute of Science and Technology 7 2 141–148.
IEEE C. Keskin, “Investigations of in vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant”, J. Inst. Sci. and Tech., vol. 7, no. 2, pp. 141–148, 2017.
ISNAD Keskin, Cumali. “Investigations of in Vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus Dioscoridis L. Var. Dioscoridis L. As a Medicinal Plant”. Journal of the Institute of Science and Technology 7/2 (June 2017), 141-148.
JAMA Keskin C. Investigations of in vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant. J. Inst. Sci. and Tech. 2017;7:141–148.
MLA Keskin, Cumali. “Investigations of in Vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus Dioscoridis L. Var. Dioscoridis L. As a Medicinal Plant”. Journal of the Institute of Science and Technology, vol. 7, no. 2, 2017, pp. 141-8.
Vancouver Keskin C. Investigations of in vitro Antioxidant Activities, Elemental Compositions and Lipid Constituents of Acanthus dioscoridis L. var. dioscoridis L. as a Medicinal Plant. J. Inst. Sci. and Tech. 2017;7(2):141-8.