Detection of Ten Resistance Genes Against P. syringae pv. phaseolicola and X. axonopodis pv. phaseoli in Twelve Local Bean Varieties Using SCAR Markers

Year 2017, Volume: 7 Issue: 2, 241 - 248, 30.06.2017

İsmail Poyraz , Berru Şahin Evren Atmaca

Abstract

Bean (Phaseolus vulgaris) is an important food and source of vitamin worldwide. It is preferred
in different cooking forms in different cultures. Some bacterial and fungal diseases cause serious loss in bean
production and impend nutrition and economy. In this study, we aimed to investigate the presence of some
resistance genes against two plant diseases (Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis
pv. phaseoli) in twelve local bean varieties (Aslan, Elinda, İstanbul, Bursa, Flash, Belluga, White cordinal,
Beryl, Yunus-90, Göynük-98, Önceler-98, Eskişehir-855). We scanned four resistance genes (SR13, ST8, SH11
and SB10) for P. syringae pv. phaseolicola and six resistance genes (SAP6, BAC6, SU91, BC420, R7313 and
R4864) for X. axonopodis pv. phaseoli. PCR amplifcations were performed with specifc SCAR markers for each
resistance gene. The obtained DNA bands were scored as present or absent for the detection of resistance genes.
For comparison, the virulence rates of two plant diseases against twelve bean varieties were obtained from feld
results. We especially observed that two bean varieties (Aslan and Beryl) including four resistance genes against P.
syringae pv. phaseolicola are more resistant to this disease in feld. We revealed analysis results for all resistance
genes in twelve bean varieties and compared with resistance rates in feld. The data obtained from this study will
seriously be conduce to the improvement of resistant varieties against pathogens in bean production

Keywords

PCR, phaseolus vulgaris, plant diseases, resistance gene markers

SCAR Markörler Kullanarak On İki Yerel Fasulye Çeşidinde P. Syringae Pv. Phaseolicola ve X. Axonopodis Pv. Phaseoli’ye Karşı On Direnç Geninin Tespit Edilmesi

Abstract

Fasulye (Phaseolus vulgaris), dünya genelinde önemli bir besin ve vitamin kaynağıdır. Farklı kültürlerde
farklı yemek formları olarak tercih edilmektedir. Bazı bakteriyel ve fungal hastalıklar, fasulye üretiminde ciddi
kayıplara neden olmakta ve beslenme ve ekonomiyi tehdit etmektedir. Bu çalışmada, on iki yerel fasulye çeşidinde
(Aslan, Elinda, İstanbul, Bursa, Flash, Belluga, White cordinal, Beryl, Yunus-90, Göynük-98, Önceler-98,
Eskişehir-855), iki bitki hastalığına (Pseudomonas syringae pv. phaseolicola and Xanthomonas axonopodis pv.
phaseoli) karşı bazı direnç genleri belirlenmesini amaçlanmıştır. P. syringae pv. phaseolicola için dört direnç geni
(SR13, ST8, SH11 ve SB10) ve X. axonopodis pv. phaseoli için ise altı (SAP6, BAC6, SU91, BC420, R7313 ve
R4864) direnç geni taranmıştır. Her bir direnç geni için polimeraz zincir reaksiyonu (PCR)-çoğaltımları spesifk
SCAR belirteçler ile gerçekleştirilmiştir. Direnç genlerinin tespiti için elde edilen DNA bantları, var veya yok olarak
kaydedilmiştir. Karşılaştırma yapmak için, iki bitki hastalığına karşı hastalık yapma oranları tarla verilerinden elde
edilmiştir. Özellikle, P. syringae pv. phaseolicola’ya karşı dört direnç geni içeren iki fasulye türünün (Aslan ve
Beryl), tarlada bu hastalığa karşı daha dirençli olduğunu gözlenmiştir. On iki fasulye çeşidindeki tüm direnç genleri
için analiz sonuçlarını ortaya konulmuş ve tarladaki direnç oranları ile karşılaştırılmıştır. Bu çalışmadan elde edilen
veriler, fasulye üretiminde patojenlere karşı dirençli çeşitlerin geliştirilmesine ciddi bir katkı sağlayacaktır

Keywords

Bitki Hastalıkları, direnç gen belirteçleri, pcr, phaseolus vulgaris

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