Determination of Antifungal and Antioxidant Activities of Salvia tomentosa Mill

Year 2017, Volume: 7 Issue: 4, 17 - 21, 31.12.2017

Yusuf Bayan Nusret Genç

Abstract

In this study, was determined antifungal and antioxidant activity of Salvia tomentosa. The essential

oil of Salvia tomentosa was determined of the percentages of mycelium inhibition agains plant pathogenic fungi

Rhizoctonia solani and Alternaria solani 40.63% and 60.13% respectively. To determine the antioxidant capacity,

Free radical reduction (scavenging), iron reduction power (FRAP), copper reduction power (CUPRAC) and free

radical cations reduction (TEAC), total phenolic (TP) and total flavonoids (TF) tests were performed. The results

of the tests, scavenging activity DPPH lC50 15.08±1.28 (μg mL-1), FRAP 1.81±0.27 mmol TE g-1 extract, CUPRAC

5.57±0.20 mmol TE g-1 extract and TEAC ise 3.30±0.33 lC50 (μg mL-1) was determined. Total phenolic and total

flavonoid was determined to be extracted content 131.34±8.39 mg GAE g-1 of extract and 35.05±2.82 QA g-1

respectively.

Keywords

Antifungal, antioxidant, Salvia tomentosa, total phenolic, essential oil

Salvia tomentosa Mill’in Antifungal ve Antioksidant Aktivitesinin Belirlenmesi

Abstract

Bu çalışma, Salvia tomentosa’nın antifungal ve antioksidant aktivitesi belirlenmesi amacıyla yürütülmüştür.

Salvia tomentosa’nın uçucu yağı bitki patojeni fungus olan, Rhizoctonia solani ve Alternaria solani ‘nin miselyum

gelişim sırasıyla % 40.63 ve % 60.13 engellemiştir. Antioksidan kapasitesini belirlemek için, Serbest Radikal

Giderme (DPPH), Demir İndirgeme Gücü (FRAP), Bakır İndirgeme Gücü (CUPRAC) ve Serbest Katyon Radikali

Giderme (TEAC) antioksidan aktivite testleri ile Toplam Fenolik (TP) ve Toplam Flavonoid (TF) miktarı analizleri

yapılmıştır. Yapılan antioksidan testleri sonucunda, DPPH lC50 15.08±1.28 (μg mL-1), FRAP 1.81±0.27 mmol TE g-1

eksrakt, CUPRAC 5.57±0,20 mmol TE g-1 ekstrakt ve TEAC ise 3.30±0.33 lC50 (μg mL-1) olarak belirlenmiştir.

Toplam fenolik ve toplam flavanoid içeriği sırasıyla 131.34±8.39 mg GAE g-1 ekstrakt ve 35.05±2.82 mg QE g-1

ekstrakt olarak tespit edilmiştir.

Keywords

Antifungal, antioksidant, Salvia tomentosa, toplam fenolik, uçucu yağ

References

• Chang C.-C,Yang MH, Wen HM. Chern JC 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods; J. FoodDrug Analysis, 10: 178-182.
• Erdogan-Orhan I, Baki E, Senol S, Yilmaz G 2010. Sage-called plant species sold in Turkey and their antioxidant activities. J Serb Chem Soc 75: 1491–1501.
• Esquivel B, Sanchez AA, Aranda E. 2000.Natural Products of Agricultural Interest from Mexican Labiatae. In: Shahidi F. and Ho CH. eds. Phytochemicals and Phytopharmaceuticals. AOCS Press: 371-385.
• Haznedaroğlu MZ, Karabay NU, Zeybek U 2001. Antibacterial activity of Salvia tomentosa essential oil. Fitoterapia. 72: 829-831.
• Liyana-Pathirana CM, F Shahidi 2005. Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. J. Agric. Food Chem., 53: 2433-2440.
• Masaki H, Sakaki S, Atsumi T, Sakurai H 1995. Activeoxygen scavenging activity of plant extracts. Biological and Pharmacological Bulletin, 18(1): 162–166.
• Oyaızu M, 1986. Studies on product of browning reaction prepared from glucose amine. Japan Journal of Nutrition, 44: 307-315.
• Papageorgiou V, Gardeli C, Mallouchos A, Papaioannou M, Komaitis M 2008. Variation of the chemical profile and antioxidant behavior of Rosmarinus officinalis L. and Salvia fruticosa Miller grown in Greece. J Agr Food Chem 56: 7254–7264.
• Re R, Pellegrini N, Protrggente A, Pannala A, Yang, M, Rice-Evans C 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Madicine, 26: 1231-1237.
• Singleton VL, Orthofer R, Lamuela-Raventos RM 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folinciocalteu reagent. Methods in Enzymology, 299: 152-178.
• Tepe B, Daferera D, Sokmen A, Sokmen M, Polissiou M 2005. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae). Food Chemistry 90: 333–340.
• Tepe B, Daferera D, Sokmen A, Sokmen M, Polissiou Moschos 2005. Antimicrobial and antioxidant activities of the essential oil and various extracts of Salvia tomentosa Miller (Lamiaceae) Food Chemistry. 90 :333–340.
• Ulubelen A 2003. Cardioactive and antibacterial terpenoids from some Salvia species. Phytochemistry, 64: 395–399.
• Ulubelen A, Topcu G, Bozok-Johansson C 1997. Norditerpenoids and diterpenoids from Salvia multicaulis with antituberculous activity. J Nat Prod 60: 1275-1280.
• Yılar, M, Kadıoglu I 2016. Antifungal Activities of some Salvia Species Extracts on Fusarium oxysporum f. sp. radicis- lycopersici (Forl) Mycelium Growth In-vitro. Egyptian Journal of Biological Pest Control, 26(1): 115-118