Antioxidant Activity of Fruit Extracts of Prangos ferulacea (L.) Lindl. from Turkey

Year 2017, Volume: 7 Issue: 4, 249 - 256, 31.12.2017

Cüneyt Cesur Belgin Coşge Şenkal Cennet Yaman Tansu Uskutoğlu Murat Koç

Abstract

It was aimed to determine anioxidant activity, and amount of total phenolic and flavonoid compounds

of methanolic and ethanolic extracts of P. ferulaceae (L.) Lindl. fruits from Turkey. The fruits were collected at

two different times, in May and July 2014. The total phenolic content of the extracts determined according to the

Folin-Ciocalteu Reactifi (FCR) method was calculated as mg Gallic acid equivalent (GAE) g-1 extract. The total

flavonoid content of the extracts was expressed as mg Quercetin (QE) g-1 extract. The percentage of antioxidant

activity of each samples was assessed by DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical assay. Total

phenolic and flavonoid contents of the extracts were determined to range from 11.750 to 79.111 mg GAE g-1 extract

and from 2.266 to 9.089 mg QE g-1 extract, respectively. According to the results of DPPH test, all fruit extracts

exhibited a slightly antioxidant activity.

Keywords

DPPH, Prangos ferulaceae, total flavonoid, total phenolic

Türkiye’deki Prangos ferulacea (L.) Lindl.’nin Meyve Özütlerinin Antioksidan Aktivitesi

Abstract

Türkiye’de doğal olarak yetişen P. ferulacea (L.) Lindl.’in metanol ve etanol ile hazırlanan meyve

özütlerinin toplam fenolik ve flavonoit miktarları ile antioksidan aktiviteleri belirlendi. Meyveler Mayıs ve Haziran

2014’de toplandı. Ekstraktların toplam fenolik içerikleri Folin-Ciocalteu Reactifi (FCR) yöntemine göre mg Gallik

asit eşdeğeri (GAE) g-1 ekstrakt olarak hesaplandı. Ekstraktların toplam flavonoit içeriği mg Kuarsetin (QE) g-1

ekstrakt olarak ifade edildi. Her bir örneğin antioksidan aktivite yüzdesi DPPH (2,2-diphenyl-1-picryl-hydrazylhydrate)

ile değerlendirildi. Özütlerin toplam fenolik ve flavonoit içerikleri sırasıyla 11.750-79.111 mg GAE g-1

ile 2.266-9.089 mg QE g-1 olarak belirlendi. DPPH testi sonuçlarına göre, tüm meyve özütlerinin zayıf antioksidan

aktiviteye sahip olduğu tespit edildi.

Keywords

DPPH, Prangos ferulaceae, toplam fenolik, toplam flavonoit

References

• Abdelhady MIS, Motaal AA, Beerhues L, 2011. Total Phenolic Content and antioxidant activity of standardized extracts from leaves and cell cultures of three Callistemon species. American Journal of Plant Sciences, 2:847-850.
• Acıkgoz N, Ilker E, Gokcol A, 2004. Biyolojik araştırmaların bilgisayarda değerlendirmeleri. Ege University Publication No:2, Izmir.
• Ahmed J, Guvenc A, Kucukboyaci N, Baldemir A, Coskun M, 2011. Total phenolic contents and antioxidant activities of Prangos Lindl. (Umbelliferae) species growing in Konya province (Turkey). Turk J Biol.,35:353-360.
• Akhgara M, Pahlavanzadeh-Irana S, Lotfı-Anarıb P, Faghıhızarandıc A, 2011. Composition of essential oils of fruits and leaves of Prangos ferulacea (L.) Lindl. growing wild in Iran. Trends in Modern Chemistry, 1:1-4.
• Arvouet-Grand A, Vennat B, Pourrat A, Legret P, 1994. Standardisation d’un extrait de propolis et identification des principaux constituants. J. de Pharmacie de Belgique, 49:462-468.
• Baser KHC, Ermin N, Adiguzel N, Aytac Z, 1996. Composition of the essential oil of Prangos ferulaceae (L.) Lindl. J. Essent. Oil Res., 8:297-298.
• Baser KHC, Ozek T, Demirici B, Duman H, 2000. Composition of essential oil of Prangos heyniae. H. Duman et M.F. Watson, a new endemic from Turkey. Flavour Fragrance Journal,15:47–49.
• Baydar H, 2013. Tıbbi ve Aromatik Bitkiler Bilimi ve Teknolojisi. Süleyman Demirel Üniversitesi, Ziraat Fakültesi, Yayın No:51.
• Bendini A, Cerretani L, Pizzolante L, et al, 2006. Phenol content related to antioxidant and antimicrobial activities of Passiflora spp. extracts. European Food Research and Technology, 223:102-109.
• Coruh N, Sagdicoglu Celep AG, Ozgokce F, 2007. Antioxidant properties of Prangos ferulacea (L.) Lindl., Chaerophyllum macropodum Boiss. and Heracleum persicum Desf. from Apiaceae family used as food in Eastern Anatolia and their inhibitory effects on glutathione-S-transferase. Food Chemistry, 100:1237-1242.
• Damyeh MS, Niakousari M, 2016. Impact of ohmic-assisted hydrodistillation on kinetics data, physicochemical and biological properties of Prangos ferulacea Lindle. essential oil: Comparison with conventional hydrodistillation. Innovative Food Science and Emerging Technologies, 33: 387–396.
• Dlugosz A, Lembas-Bogaczyk J, Lamer-Zarawsko E. 2006, Antoxid increases ferric reducing antioxidant power (FRAP) even stronger than vitamin C. Acta Pol. Pharm., 63:446-448.
• Gezer K, Duru ME, Kivrak I, Tırkoglu A, Mercan N, Turkoglu H, Gulcan S, 2006. Free-radical scavenging capacity and antimicrobial activity of wild edible mushroom of Turkey. African Journal of Biotechnology, 5:1924-1928.
• Guner A, Aslan S, Ekim T, Vural M, Babac MT, 2012. Türkiye bitkileri listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, İstanbul.
• Harish R, Shivanandappa T, 2006. Antioxidant activity and hepatoprotective potential of Phyllanthus niruri. Food Chemistry, 95:180-185.
• Hassimotto NMA, Genovese MI, Lajolo FM, 2005. Antioxidant activity of dietary fruits, vegetables, and commercial frozen fruit pulps. Journal of Agricultural and Food Chemistry, 53:2928-2935.
• Kafash-Farkhad N, Asadi-Samani M, Rafieian-Kopaei M, 2013.A review on phytochemistry and pharmacological effects of Prangos ferulacea (L.) Lindl. Life Science Journal, 10:360-367.
• Mammadov R, 2014. Tohumlu Bitkilerde Sekonder Metabolitler. Nobel Akademik Yayıncılık Eğitim Danışmanlık Tic. Ltd. Şti.Yayın No:841.
• Marotti M, Piccaglia R, Gıovanelli E, Deans SG, Eaglesham E,1994. Effects of variety and ontogenic stage on the essential oil composition and biological activity of fennel (Foeniculum vulgare Mill.). Journal of Essential Oil Research, 6:57-62.
• Mirzaei HH, Meshkatalsadat MH, Soheilivand S, 2007.Determination of essential oil composition of Prangos acaulis (DC) Bornm obtained by hydrodistillation and supercritical fluid extraction methods. Journal of Applied Sciences, 7:2535-2538.
• Mneimne M, Baydoun S, Nemer N, Apostolides NA, 2016.Chemical composition and antimicrobial activity of essential oils isolated from aerial parts of Prangos asperula Boiss. (Apiaceae) growing wild in Lebanon. Medicinal&Aromatic Plants, 5:3. 5:242.doi.10.4172/2167-0412.1000242.
• Ogut S, 2014. Importance of natural antioxidants. Journal of Adnan Menderes University Agricultural Faculty, 11:25-30.
• Razavi SM, Zahri S, Zarrini G, Nazemıyeh H, Mohammadı S, 2009. Biological activity of quercetin-3-O-glucoside, a known plant flavonoid. Russian Journal of Bioorganic Chemistry, 35:376-378.
• Razavi SM, Nazemiyeh H, Zarrini G, Asna-Asharii S, Dehghan G, 2010. Chemical composition and antimicrobial activity of essential oil of Prangos ferulaceae (L.) Lindl from Iran. Nat Prod Res., 24:530-533.
• Sajjadı SE, Mehregan I, 2003. Chemical composition of of the essential oil of Prangos asperula Boiss. subsp. haussknechtii (Boiss.) Herrnst. et Heyn fruits. Daru, 11:79-81.
• Shirazi OU, Khattak MAK, Shukri NAM, MN Nasyriq A, 2014. Determination of total phenolic, flavonoid content and free radical scavenging activities of common herbs and spices. Journal of Pharmacognosy and Phytochemistry, 3:104-108.
• Singleton VL, Orthofer R, Lamuela-Raventos RM, 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol, 299:152-178.
• Soni U, Brar S, Gauttamm VK, 2015. Effect of seasonal variation on secondary metabolites of medicinal plants. International Journal of Pharmaceutical Sciences and Research, 6:3654-3662.
• Tirzitis G, Bartosz G, 2010. Determination of antiradical and antioxidant activity: basic principles and new insights. Acta Biochimica Polonica, 57:139-142.
• TUBIVES, 2011.Turkish Plants Data Servicehttp//www.tubives.com. (Erişim tarihi: 01.05.2017).Wojdyło A, Oszmian J, Czemerys R, 2007. Antioxidant activity and phenolic compounds in selected herbs. Food Chemistry, 105: 940-949.