Phaseolus vulgaris’te Genomik Kararsızlık ve DNA Metilasyonunu İndükleyen Piklorama karşı Putresinin Koruyucu Rolü

Year 2017, Volume: 7 Issue: 4, 271 - 279, 31.12.2017

Mahmut Sinan Taşpınar Esra Arslan Murat Aydın Güleray Ağar

Abstract

Pikloram (4-amino-3-5-6-trikloropikolinik asit) önemli bir sentetik oksin olup, tarımda en çok kullanılan

herbisitlerden biridir. Pikloram kullanımı, ekosistem ve insan sağlığı için potansiyel bir tehlike oluşturmaktadır.

Bir poliamin çeşidi olan putresinin bitki metabolizmasında; membran stabilitesinin korunması, serbest radikallerin

uzaklaştırılması ve nükleik asit ve protein sentezi gibi rolü vardır. Bu çalışma, pikloram uygulanan Phaseolus

vulgaris’ teki DNA hasar düzeyleri ve DNA metilasyon değişiklikleri ile putresinin bu değişimler üzerinde

koruyucu etkisinin olup olmadığının belirlenmesini amaçlamıştır. DNA hasar düzeylerini ve DNA metilasyon

değişimlerini belirlemek için Rastgele Çoğaltılmış Polimorfik DNA (RAPD) ve Çift Restriksiyon Enzim Kesimi-

Rastgele Çoğaltım (CRED-RA) kullanılmıştır. Sonuçlar, tüm pikloram dozlarının (5, 10, 20 ve 40 mg L-1) RAPD

profil değişikliklerini (DNA hasarının) artırdığını ve Genomik Kararlılık Stabilitesini (GTS) azalttığını ve ayrıca

DNA hipometilasyonunun oluştuğunu göstermiştir. Bununla birlikte, kullanılan tüm putresin konsantrasyonları

(0.01, 0.1 ve 1 ppm) pikloramın bu zararlı etkilerini azaltmıştır. Sonuç olarak, putresin, bitkilerdeki kimyasal

mutajenlere karşı genotoksik hasarı azaltmak için bir alternatif olabilir.

Keywords

DNA metilasyonu, genomik kararsızlık, pikloram, putresin

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