Research Article
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Determination of Genetic Diversity in Apodemus mystacinus (Mammalia: Rodentia) based on SSRs

Year 2019, Volume: 9 Issue: 2, 1098 - 1108, 01.06.2019
https://doi.org/10.21597/jist.409142

Abstract

The aim of this study is to determine the genetic diversity of species in Rocky mouse, Apodemus mystacinus, using SSR (the simple sequence repeat) loci and to reveal the reasons of this variations, if any. In this study, 69 A. mystacinus samples were studied collected from 18 localities in Turkey. 7 SSR loci were used to determine the genetic diversity of A. mystacinus. As a result of this study, A. mystacinus includes 2 genetic groups that indicate the presence of two subspecies as A. m. mystacinus that is distributed in western Anatolia and A. m. euxinus in eastern Anatolia. This result also supports that one of the micro refugium areas is eastern Turkey and the other western and southern Turkey.

Supporting Institution

Ankara University, Scientific Research Projects Coordination Unit

Project Number

14B0430001

Thanks

This study was supported by Ankara University, Scientific Research Projects Coordination Unit (BAPRO: 14B0430001). We would like to thank to Prof. Dr. Reyhan ÇOLAK and Prof. Dr. Ercüment ÇOLAK for sharing their valuable contributions, equipments and collecting specimens in this study.

References

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Apodemus mystacinus’un (Mammalia: Rodentia) Genetik Çeşitliliğinin SSR ile Belirlenmesi

Year 2019, Volume: 9 Issue: 2, 1098 - 1108, 01.06.2019
https://doi.org/10.21597/jist.409142

Abstract

Bu çalışmanın amacı, SSR (basit dizi tekrarları) lokuslarını kullanarak kayalık faresinin, Apodemus mystacinus, genetik çeşitliliğini belirlemek ve varsa bu çeşitliliğin nedenleri ortaya koymaktır. Bu çalışmada, Türkiye'deki 18 lokaliteden toplanan 69 A. mystacinus örneği çalışılmıştır. A. mystacinus'un genetik çeşitliliğini belirlemek için 7 SSR lokusu kullanılmıştır. Bu çalışmanın sonucuna göre A. mystacinus, iki alttürün varlığını gösteren 2 genetik grup içermektedir; Anadolu’nun batısında A. m. mystacinus ve Anadolu’nun doğusunda A. m. euxinus. Bu sonuç aynı zamanda mikro sığınak alanlarından birinin Türkiye'nin doğusunda, diğerinin ise batısında ve güneyinde olduğunu desteklemektedir.

Project Number

14B0430001

References

  • Allegrucci G, Ketmaier V, Di Russo C, Rampini M, Sbordoni V, Cobolli M, 2017. Molecular phylogeography of Troglophilus cave crickets (Orthoptera, Rhaphidophoridae): A combination of vicariance and dispersal drove diversification in the East Mediterranean region. Journal of Zoological Systematic and Evolutionary Research, 55: 310–325.
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  • Burgos-Paz W, Ceron-Munoz M, SolartePortilla C, 2011. Genetic diversity and population structure of the Guinea pig (Cavia porcellus, Rodentia, Caviidae) in Colombia. Genetics and Molecular Biology, 34: 711-718.
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  • Çolak E, Yiğit N, Çolak R, Sözen M, Özkurt Ş, Kankılıç T, 2004. Taxonomic Status and Distribution of Apodemus mystacinus (Danford and Alston, 1877) (Mammalia: Rodentia) in Turkey. Turkish Journal of Zoology, 28: 285-294. Çolak R, Kandemir İ, Çolak E, Yiğit N, 2007. Esterase Variation in Rocky Mouse, Apodemus mystacinus (DanforD and alston, 1877) (Mammalia: Rodentia)
in Turkey. Acta Zoologica Bulgarica, 59 (1): 47-52.
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  • Deffontaine V, Libois R, Kotlik P, 2005. Beyond the Mediterranean peninsulas: Evidence of Central European glacial refugia for a temperate forest mammal species, the bank vole (Clethrionomys glareolus). Molecular Ecology, 14: 1727–1739.
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  • Fritz U, Ayaz D, Hundsdörfer AK, Kotenko T, Guicking D, Wink M, Tok V, Çiçek K, Buschbom J, 2009. Mitochondrial diversity of European pond turtles (Emys orbicularis) in Anatolia and Ponto-Capian Region: Multiple old refuges, hotspot of extant diversification and critically endangered endemics. Organisms Diverity and Evolution, 9: 100-114.
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  • Gortat T, Rutkowski R, Gryczynska-Siemiatkowska A, Kozakiewicz A, Kozakiewicz M, 2013. Genetic structure in urban and rural populations of Apodemus agrarius in Poland. Mammalian Biology, 78: 171–177.
  • Gündüz I, Jaarola M, Tez C, Yeniyurt C, Polly PD, Searle JB, 2007. Multigenic and morphometric differentiation of ground squirrels (Spermophilus, Sciuridae, Rodentia) in Turkey, with a description of a new species. Molecular Phylogenetics and Evolution, 43: 3-6.
  • Gür H, 2016. The Anatolian diagonal revisited: Testing the ecological basis of a biogeographic boundary. Zoology in the Middle East, 62:189–199.
  • Gür H, 2017. Anadolu diyagonali: Bir biyocoğrafi sınırın anatomisi. Kebikeç, İnsan Bilimleri İçin Kaynak Araştırmaları Dergisi, 43: 177-187. Gür H, Perktaş U, Gür MK, 2017. Do climate-driven altitudinal range shifts explain the intraspecific diversification of a narrow ranging montane mammal, Taurus ground squirrels?. Mammal Research, 63:1-15.
  • Gvozdik V, Moravec , Klütsch C, Kotlik P, 2010. Phylogeography of the Middle Eastern tree frogs (Hyla, Hylidae, Amphibia) as inferred from nuclear and mitochondrial DNA variation, with a description of a new species. Molecular Phylogenetics and Evoution, 55: 1146–1166.
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  • Hurner H, Krystufek B, Sara M, Ribas A, Ruc T., Sommer R, 2010. Mitochondrial phylogeography of the edible dormouse (Glis glis) in the western Palearctic region. Journal of Mammalogy, 91(1): 233–242.
  • İbiş O, Koepfli K-P, Özcan S, Tez C, 2018. Genetic analysis of Turkish martens: Do two species of the genus Martes occur in Anatolia?. Zoologica Scripta, 47: 390–403.
  • Jaarola M, Martınkova N, Gündüz İ, Brunhoff C, Zima J, Nadachowski A. et al., 2004. Molecular phylogeny of the speciose vole genus Microtus (Arvicolinae, Rodentia) inferred from mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 33: 647–663.
  • Jandzik D, Jablonski D, Zinenko O, Kukushkin OV, Moravec J, Gvoždík V, 2018. Pleistocene extinctions and recent expansions in an anguid lizard of the genus Pseudopus. Zoologica Scripta, 47:21–32.
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  • Macholan M, Munclinger P, Sugerkova M, Dufkova P, Bimova B, Bozikova E, Zima J, Pialek J, 2007. Genetic Analysis Of Autosomal And X-Linked Markers Across A Mouse Hybrid Zone. Evolution, 61(4): 746–771.
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  • Michaux J, Bellinvia E, Lymberakis P, 2005.Taxonomy, evolutionary history and biogeography of the broad-toothed field mouse (Apodemus mystacinus) in the eastern Mediterranean area based on mitochondrial and nuclear genes. Biological Journal of the Linnean Society, 85: 53–63.
  • Michaux JR, Chevret P, Filippucci MG, Macholán M, 2002. Phylogeny of the genus Apodemus with a special emphasis on the subgenus Sylvaemus using the nuclear IRBP gene and two mitochondrial markers: cytochrome b and 12S rRNA. Molecular Phylogenetics and Evolution, 23: 123–136.
  • Michaux JR, Filippucci M-G, Libois R, 2004. Phylogeographic history of the yellow-necked fieldmouse (Apodemus flavicollis) in Europe and in the Near and Middle East. Molecular Phylogenetics and Evolution, 32: 188–198.
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There are 63 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Moleküler Biyoloji ve Genetik / Moleculer Biology and Genetic
Authors

Gül Olgun Karacan 0000-0002-7160-5766

Dilek Beteş This is me 0000-0002-3380-5804

Project Number 14B0430001
Publication Date June 1, 2019
Submission Date March 26, 2018
Acceptance Date December 22, 2018
Published in Issue Year 2019 Volume: 9 Issue: 2

Cite

APA Olgun Karacan, G., & Beteş, D. (2019). Determination of Genetic Diversity in Apodemus mystacinus (Mammalia: Rodentia) based on SSRs. Journal of the Institute of Science and Technology, 9(2), 1098-1108. https://doi.org/10.21597/jist.409142
AMA Olgun Karacan G, Beteş D. Determination of Genetic Diversity in Apodemus mystacinus (Mammalia: Rodentia) based on SSRs. J. Inst. Sci. and Tech. June 2019;9(2):1098-1108. doi:10.21597/jist.409142
Chicago Olgun Karacan, Gül, and Dilek Beteş. “Determination of Genetic Diversity in Apodemus Mystacinus (Mammalia: Rodentia) Based on SSRs”. Journal of the Institute of Science and Technology 9, no. 2 (June 2019): 1098-1108. https://doi.org/10.21597/jist.409142.
EndNote Olgun Karacan G, Beteş D (June 1, 2019) Determination of Genetic Diversity in Apodemus mystacinus (Mammalia: Rodentia) based on SSRs. Journal of the Institute of Science and Technology 9 2 1098–1108.
IEEE G. Olgun Karacan and D. Beteş, “Determination of Genetic Diversity in Apodemus mystacinus (Mammalia: Rodentia) based on SSRs”, J. Inst. Sci. and Tech., vol. 9, no. 2, pp. 1098–1108, 2019, doi: 10.21597/jist.409142.
ISNAD Olgun Karacan, Gül - Beteş, Dilek. “Determination of Genetic Diversity in Apodemus Mystacinus (Mammalia: Rodentia) Based on SSRs”. Journal of the Institute of Science and Technology 9/2 (June 2019), 1098-1108. https://doi.org/10.21597/jist.409142.
JAMA Olgun Karacan G, Beteş D. Determination of Genetic Diversity in Apodemus mystacinus (Mammalia: Rodentia) based on SSRs. J. Inst. Sci. and Tech. 2019;9:1098–1108.
MLA Olgun Karacan, Gül and Dilek Beteş. “Determination of Genetic Diversity in Apodemus Mystacinus (Mammalia: Rodentia) Based on SSRs”. Journal of the Institute of Science and Technology, vol. 9, no. 2, 2019, pp. 1098-0, doi:10.21597/jist.409142.
Vancouver Olgun Karacan G, Beteş D. Determination of Genetic Diversity in Apodemus mystacinus (Mammalia: Rodentia) based on SSRs. J. Inst. Sci. and Tech. 2019;9(2):1098-10.