Akkeçilerde İnsülin Benzeri Büyüme Faktörü-I’in (IGF-I) Laktasyon Dönemindeki Değişimi

Year 2019, Volume: 9 Issue: 2, 1193 - 1200, 01.06.2019

Naciye Melis Terzi , Erkan Pehlivan , Gürsel Dellal

https://doi.org/10.21597/jist.469393

Abstract

Bu araştırmada, Ankara Üniversitesi Ziraat Fakültesi Zootekni Bölümü Hayvancılık İşletmesinde yetiştirilen 6 baş 2 yaşlı ve 5 baş 3 yaşlı olmak üzere toplam 11 baş dişi Akkeçide laktasyon döneminde IGF-I’in değişimi incelenmiştir. Çalışmada, IGF-I konsantrasyonlarını belirlemek için laktasyon dönemi boyunca keçilerin boyun toplardamarından her iki haftada bir düzenli olarak kan örnekleri alınmıştır. Kan örnekleri 4000 devir/dak’da 5 dakika süreyle santrifüj edilmiş ve serumlar -20 °C’de laboratuvar analizleri gerçekleştirilene kadar muhafaza edilmiştir. Kan serumunda IGF-I analizleri enzim immunoassay (EIA) yöntemiyle gerçekleştirilmiştir. Araştırmanın gerçekleştirildiği döneme ait meteorolojik veriler Meteoroloji Genel Müdürlüğü’nden temin edilmiş ve bu değerlerden sıcaklık-nem indeksi (SNİ) hesaplanmıştır. Araştırmada, IGF-I konsantrasyonları bakımından dönemler ve yaş grupları arasındaki farklılıklar, tekrarlanan ölçümlü varyans analizi tekniği ile değerlendirilmiştir. İstatistik analiz sonucunda, sadece incelenen dönemler arasındaki farklılıklar istatistik olarak önemli (P<0.01) bulunmuştur. Sonuç olarak, Akkeçilerde laktasyon döneminde, IGF-I konsantrasyonlarının fotoperiyot değişimlerinden önemli düzeyde etkilendiği ve gün uzunluğundaki artışa bağlı olarak IGF-I salınımının arttığı belirlenmiştir.

Keywords

Akkeçi, IGF-I, laktasyon, fotoperiyot, sıcaklık, SNİ

Supporting Institution

Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Project Number

16L0447003

Thanks

Bu çalışma, Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından “16L0447003” kodlu proje ile desteklenmiştir.

Change of Insulin-Like Growth Factor-1 in Lactation Period in White Goats

Abstract

In this study, change of IGF-I in lactation period was investigated on 11 heads female white goat (6 heads 2 aged and 5 heads 3 aged) raised at the farm of Department of Animal Science, Faculty of Agriculture, Ankara University, Turkey. To determine IGF-I concentrations, the blood samples were regularly taken from jugular vein of goats in every two weeks during lactation period. The blood samples were centrifuged at 4000xg for 5 min. and serum was stored at -20 °C until analysis time. IGF-I analysis in the blood serum were performed by enzyme immunoassay (EIA) method. Climatic values of the experimental period were obtained from the local meteorological service and the temperature-humidity index (THI) was calculated. In the study, the observed IGF-I concentrations with respect to periods and age groups was analyzed by the repeated measures of ANOVA analysis. Significant differences (P<0.01) were found among the periods for IGF-I concentrations. In conclusion IGF-I concentrations were significantly influenced by photoperiod changes and IGF-I releases were depend on increased length of day during lactation period in White goats.

Keywords

IGF-I, White goat, lactation, photoperiod, temperature, THI

Project Number

16L0447003

References

• Aggarwal A, Upadhyay R, 2013. Heat Stress and Animal Productivity, DOI 10.1007/978-81-322-0879-2_3, Springer, India.
• Anonim, 2016. T. C. Orman ve Su İşleri Bakanlığı, Meteoroloji Genel Müdürlüğü, Veri Kontrol ve İstatistik Şube Müdürlüğü, Ankara.
• Baştürk E, 2007. Nodüler guatr oluşumunda insülin benzeri büyüme faktörünün (IGF-1) rolü. T.C. Sağlık Bakanlığı, Dr. Lütfi Kırdar Kartal Eğitim ve Araştırma Hastanesi, 1. Genel Cerrahi Kliniği, Uzmanlık Tezi.
• Dahl GE, Elsasser TH, Capuco AV, Erdman RA, Peters RR, 1997. Effects of long day photoperiod on milk yield and circulating insulin-like growth factor-1. J Dairy Sci, 80: 2784–2789.
• Dahl GE, Buchanan BA, Tucker HA, 2000. Photoperiodic effects on dairy cattle: A review. J Dairy Sci, 83: 885–893.
• Faulkner A, 1999. Changes in plasma and milk concentrations of glucose and IGF-1 in response to exogenous growth hormone in lactating goats. Journal of Dairy Research, 66: 207-214.
• Gürbüz F, Başpınar E, Çamdeviren H, Keskin S, 2003. Tekrarlanan ölçümlü deneme düzenlerinin analizi. Yüzüncü Yıl Üniversitesi Matbaası, Van.
• Hadsell DL, Bonnette SG, Lee AV, 2002. Genetic manipulation of the IGF-I axis to regulate mammary gland development and function. J Dairy Sci, 85: 365-377.
• Hamzaoui S, Salama KAA, Albanell E, Such X, Caja G, 2013. Physiological responses and lactational performances of late-lactation dairy goats under heat stress conditions. J Dairy Sci, 96: 6355–6365.
• Hashizume T, Takahashi Y, Numata M, Sasaki K, Ueno K, Ohtsuki K, Kawai M, Ishii A, 1999. Plasma Profiles of Growth Hormone, Prolactin and Insulin-like Growth Factor-I during Gestation, Lactation and the Neonatal Period in Goats. J Reprod Dev, 45: 273–281.
• Jin J, Sawai K, Hashizume T, 2013. Effects of photoperiod on secretory patterns of growth hormone in adult male goats. Animal Science Journal, 84: 790–797.
• Ma H, Zhang W, Song WH, Sun P, Jia ZH, 2012. Effects of tryptophan supplementation on cashmere fiber characteristics, serum tryptophan, and related hormone concentrations in cashmere goats. Domestic Animal Endocrinology, 43: 239–250.
• Mabjeesh SJ, Gal-Garber O, Shamay A, 2007. Effect of Photoperiod in the Third Trimester of Gestation on Milk Production and Circulating Hormones in Dairy Goats. J Dairy Sci, 90: 699–705.
• Magistrelli D, Valli A, Rosi F, 2005. Insulin and IGF-1 in goat milk: influence of the diet. Ital J Anim Sci, 4 (2): 386-388.
• Marai IFM, Ayyat MS, Abd El-Monem UM, 2001. Growth performance and reproductive traits at first parity of New Zealand White female rabbits as affected by heat stress and its alleviation under egyptian conditions. Tropical Animal Health and Production, 33: 451-462.
• Marai IFM, El-Darawany AA, Fadiel A, Abdel-Hafez MAM, 2007. Physiological traits as affected by heat stress-a review. Small Ruminant Research, 71: 1-12.
• McGuire MA, Vicini JL, Bauman DE, Veenhuizent JJ, 1992. Insulin-like growth factors and binding proteins in ruminants and their nutritional regulation. J Anim Sci, 70: 2901-2910.
• Moyes TE, Stockdale CR, Humphyrs S, Macmillan KL, 2003. Differences in plasma concentration of insulin-like growth factor-1 between pregnant and nonpregnant dairy cows. Reproduction, Fertility and Development, 15: 22.
• Nielsen MO, Skakkebaek NE, Giwercman A, 1990. Insulın-like growth factor I (somatomedın C) in goats during normal lactation and in response to somatotropin treatment. Comp Biochem Physiol, 95A (2): 303-306.
• Noyan A, 1988. Fizyoloji. Meteksan Ltd., 5. Baskı, S. 1007. Ankara.
• Prosser CG, 1996. Insulin-like growht factors in milk and mammary gland. Journal of Mammary Gland Biology and Neoplasia, 1 (3): 297-306.
• Rasouli S, Abdolmohammadi A, Zebarjadi A, Mostafae A, 2017. Evaluation of polymorphism in IGF-I and IGFBP-3 genes and their relationship with twinning rate and growth traits in Markhoz goats. Ann Anim Sci, 17 (1): 89-103.
• Rhind SM, McMillen SR, 1995. Seasonal Changes in Systemic Hormone Profiles and their Relationship to Patterns of Fibre Growth and Moulting in Goats of Contrasting Genotypes. Aust J Agric Res, 46: 1273-1283.
• Richards MW, Spicer LJ, Wettemann RP, 1995. Influence of diet and ambient temperature on bovine serum insulin-like growth factor-I and thyroxine: relationships with non-esterified fatty acids, glucose, insulin, luteinizing hormone and progesterone. Animal Reproduction Science 37: 267-279.
• Rosi F, Rapetti L, 2004. Bioactive components in goat milk and plasma. Journal of Animal and Feed Sciences, 13 (1): 701-704.
• Spicer LJ, Buchanan BA, Chapin LT, Tucker HA, 1994. Effect of 4 months of exposure to various durations of light on serum insulin-like growth factor-1 (IGF-1) in prepubertal Holstein heifers. J Anim Sci, 72 (1): 178.
• Suttie JM, Breier BH, Gluckman PD, Littlejohn RP, Webster JR, 1992. Effects of melatonin implants on insulin like growth factor 1 in male red deer (Cervus elaphus). Gen Comp Endocrinol, 87: 111–119.
• Squires EJ, 2003. Applied animal endocrinology. CABI Publushing, ISBN: 0-85199-594-2, USA. pp. 234.
• Thammacharoen S, Nguyen T, Suthikai W, Chanchai W, Chanpongsang S, Chaiyabutr N, 2014. Somatotropin supplementation decreases feed intake incrossbred dairy goats during the early phase of lactation. Small Ruminant Research, 121: 368–375.
• Yılmaz B, 1999. Hormonlar ve Üreme Fizyolojisi. Feryal Matbaacılık, 1.Basım, ISBN: 975-96982-0 X, Ankara.