Year 2020, Volume 10 , Issue 1, Pages 320 - 327 2020-03-01

Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri
Synthesis, characterization, and inhibitory properties of novel N-benzylbenzimidazole-silver(I) complexes on carbonic anhydrase and polyphenol oxidase enzymes

Adem ERGÜN [1] , Mert Olgun KARATAŞ [2]


Gümüş komplekslerinin biyolojik özellikleri çok iyi bilinmesine rağmen enzim inhibisyonu özelliklerine yönelik çalışmalar oldukça sınırlıdır. Diğer taraftan benzimidazol türevlerinin anti-kanser etki mekanizmalarından birinin enzim inhibisyonu olduğu bilinmektedir. Bu nedenle bu çalışmada, beş yeni N-benzilbenzimidazol-gümüş(I) kompleksi sentezlendi, 1H NMR, 13C NMR, IR ve kütle spektroskopik yöntemleri ve elementel analiz sonuçları ile tamamen karakterize edildi. Elde edilen komplekslerin insan karbonik anhidraz I - II ve polifenol oksidaz enzimleri üzerindeki inhibisyon etkileri incelendi ve komplekslerin bu enzimlerin aktivitelerini sırasıyla 29.58-32.65 µM, 21.05-23.65 µM ve 30.38-44.45 µM’lık IC50 değerleri ile inhibe ettiği gözlendi. Gümüş bileşiklerinin insan vücudunda düşük konsantrasyonlarda toksik etki göstermediği de göz önüne alınırsa, bu çalışmada test edilen kompleksler karbonik anhidraz inhibitörü olarak glokom tedavisinde ve polifenol oksidaz inhibitörü olarak ise hiper-pigmentasyon hastalığı tedavisinde kullanılmak için umut verici adaylardır.      

The biological properties of silver complexes are well known but the studies about the enzyme inhibitory properties of them are very rare. On the other hand, it is known that one of the anticancer mechanisms of action of benzimidazole derivatives is enzyme inhibition. Therefore, in this study, five novel N-benzylbenzimidazole-silver(I) complexes were synthesized and fully characterized by 1H NMR, 13C NMR, IR, mass spectroscopic methods and elemental analyses. The inhibitory properties of the synthesized complexes were tested on the activity of human carbonic anhydrase I - II and polyphenol oxidase, and it was observed that the complexes inhibited the activity of these enzymes with the IC50 values of 29.58-32.65 µM, 21.05-23.65 µM and 30.38-44.45 µM, respectively. We suggest that by also considering the low toxicity of silver compounds to human body at low concentrations, the evaluated complexes in this study are promising agents in the treatment of glaucoma and hyperpigmentation as carbonic anhydrase and polyphenol oxidase inhibitors.

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Primary Language tr
Subjects Engineering, Chemical
Published Date Mart-2020
Journal Section Kimya / Chemistry, Kimya Mühendisliği / Chemical Engineering
Authors

Orcid: 0000-0003-4647-6058
Author: Adem ERGÜN
Institution: BALIKESİR ÜNİVERSİTESİ
Country: Turkey


Orcid: 0000-0001-8500-2088
Author: Mert Olgun KARATAŞ (Primary Author)
Institution: İNÖNÜ ÜNİVERSİTESİ
Country: Turkey


Dates

Application Date : April 9, 2019
Acceptance Date : September 3, 2019
Publication Date : March 1, 2020

Bibtex @research article { jist551498, journal = {Journal of the Institute of Science and Technology}, issn = {2146-0574}, eissn = {2536-4618}, address = {}, publisher = {Igdir University}, year = {2020}, volume = {10}, pages = {320 - 327}, doi = {10.21597/jist.551498}, title = {Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri}, key = {cite}, author = {ERGÜN, Adem and KARATAŞ, Mert Olgun} }
APA ERGÜN, A , KARATAŞ, M . (2020). Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri. Journal of the Institute of Science and Technology , 10 (1) , 320-327 . DOI: 10.21597/jist.551498
MLA ERGÜN, A , KARATAŞ, M . "Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri". Journal of the Institute of Science and Technology 10 (2020 ): 320-327 <https://dergipark.org.tr/en/pub/jist/issue/52503/551498>
Chicago ERGÜN, A , KARATAŞ, M . "Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri". Journal of the Institute of Science and Technology 10 (2020 ): 320-327
RIS TY - JOUR T1 - Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri AU - Adem ERGÜN , Mert Olgun KARATAŞ Y1 - 2020 PY - 2020 N1 - doi: 10.21597/jist.551498 DO - 10.21597/jist.551498 T2 - Journal of the Institute of Science and Technology JF - Journal JO - JOR SP - 320 EP - 327 VL - 10 IS - 1 SN - 2146-0574-2536-4618 M3 - doi: 10.21597/jist.551498 UR - https://doi.org/10.21597/jist.551498 Y2 - 2019 ER -
EndNote %0 Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri %A Adem ERGÜN , Mert Olgun KARATAŞ %T Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri %D 2020 %J Journal of the Institute of Science and Technology %P 2146-0574-2536-4618 %V 10 %N 1 %R doi: 10.21597/jist.551498 %U 10.21597/jist.551498
ISNAD ERGÜN, Adem , KARATAŞ, Mert Olgun . "Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri". Journal of the Institute of Science and Technology 10 / 1 (March 2020): 320-327 . https://doi.org/10.21597/jist.551498
AMA ERGÜN A , KARATAŞ M . Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri. Iğdır Üniv. Fen Bil Enst. Der.. 2020; 10(1): 320-327.
Vancouver ERGÜN A , KARATAŞ M . Yeni N-benzilbenzimidazol-gümüş(I) komplekslerinin sentezi, karakterizasyonu, karbonik anhidraz ve polifenol oksidaz enzimleri üzerindeki inhibitör özellikleri. Journal of the Institute of Science and Technology. 2020; 10(1): 327-320.