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Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon ve Tür Farkının Etkisi

Year 2020, Volume: 10 Issue: 1, 648 - 657, 01.03.2020
https://doi.org/10.21597/jist.596057

Abstract

Bu çalışmanın amacı Tükiye’nin farklı lokasyonlarından toplanmış bazı endemik Haplophyllum A. Juss. (H. myrtifolium, H. vulcanicum, H. pumiliforme ve H. sahinii) türlerinin toplam fenolik, toplam flavonoid ve antioksidan aktivitelerinin belirlenmesidir. Örneklerin toplam fenolik miktarı 31.8 ila 49.8 mg GAE g-1 ekstrakt arasında değişmiştir. H. vulcanicum ve H. sahinii diğer türlere göre daha yüksek toplam fenolik içeriğine sahip olmuştur. En yüksek toplam flavonoid miktarı H. myrtifolium türünün her iki lokasyonundaki örneklerinde (62.0 ve 63.0 mg KE g-1 ekstrakt), en düşük ise H. pumiliforme türünün her iki lokasyonundaki örneklerinde (36.2 ve 31.7 mg KE g-1 ekstrakt) tespit edilmiştir. Antioksidan aktivitesi olarak DPPH ve ABTS radikal kovucu aktiviteleri incelenmiştir. H. myrtifolium türünün lokasyon1 örnekleri ile H. pumiliforme türünün lokasyon2 örnekleri en yüksek ABTS radikal kovucu aktivitesi sergilemiştir (sırasıyla %78.6 ve %79.0). H. vulcanicum türünün iki lokasyonundaki örnekleri ise iki aktivite içinde en düşük değer göstermiştir. Sonuç olarak, H. pumiliforme türünün düşük fitokimyasal içerdiğine sahip olduğu, fakat yüksek oranda antioksidan aktivite sergilediği tespit edilmiştir. H. vulcanicum, H. sahini ve H. pumiliforme hakkındaki toplam biyoaktif madde içeriği ve antioksidan aktivitesi ilk kez tarafımızdan bildirilmiştir.

Supporting Institution

Selçuk üniversitesinin Bilimsel Araştırma Projeleri Koordinatörlüğüne

Project Number

13201023

Thanks

Selçuk üniversitesinin Bilimsel Araştırma Projeleri Koordinatörlüğüne (Proje no: 13201023) desteklerinden dolayı teşekkür ederiz.

References

  • Al-Rehaily AJ, Alqasoumi SI, Yusufoglu HS, Al-Yahya MA, Demirci B, Tabanca N, Wedge DE, Demirci F, Bernier UR, Becnel JJ, Temel HE, Can Baser KH, 2014. Chemical composition and biological activity of Haplophyllum tuberculatum Juss essential oil. Journal of Essential Oil Bearing Plants, 17(3): 452-459.
  • Arvouet-Grand A, Vennat B, Pourrat A, Legret P, 1994. Standardization of a propolis extract and identification of the main constituents. Journal de pharmacie de Belgique, 49: 462–468.
  • Ashraf MA, Iqbal M, Rasheed R, Hussain I, Riaz M, Arif MS, 2018. Chapter 8 - Environmental Stress and Secondary Metabolites in Plants: An Overview. Plant Metabolites and Regulation Under Environmental Stress, 153-167.
  • Awaad AS, Alothman, EAA, 2018. Antiulcer and Anti-ulcerative Colitis Activities of Haplophyllum tuberculatum (Forsskal). International Journal of Pharmacology, 14(1): 31-38.
  • Bajaj S, Urooj A, Prabhasankar P, 2006. Effect of incorporation of mint on texture, colour and sensory parameters of biscuits. International Journal of Food Properties, 9: 691-700.
  • Ben Farhat M, Landoulsi A, Chaouch-Hamada R, Sotomayor JA, Jordánc MJ, 2013. Characterization and quantification of phenolic compounds and antioxi-dant properties of Salvia species growing in different habitats. Industrial Crops and Products, 49: 904–914.
  • Deng B, Fang S, Shang X, Fu X, Yang W, 2019. Influence of genotypes and environmental factors on leaf triterpenoid content and growth of Cyclocarya paliurus. Journal of Forestry Research, 30(3): 789–798.
  • Debouba M, Khemakhem B, Zouari S, Meskine A, Gouia H, 2014. Chemical and Biological Activities of Haplophyllumtuberculatum Organic Extracts and Essential Oil. Journal of Essential Oil Bearing Plants, 17(5): 787-796.
  • Düzgüneş O, Kesici T, Gürbüz F, 1983. İstatistik Metotları 1. Anakara Üniversitesi Ziraat Fakültesi Yay. No: 862, Ankara.
  • Eissa T, González-Burgos E, Carretero M, Gómez-Serranillos M, 2014. Biologicalactivity of HPLC-characterized ethanol extract from the aerial parts of Haplophyllum tuberculatum, Pharmaceutical Biology, 52(2): 151–156.
  • Gezer K, Duru ME, Kivrak I, Turkoglu A, Mercan N, Turkoglu H, Gülcan S, 2006. Free-radical scavenging capacity and antimicrobial activity of wild edible mushroom from Turkey. African Journal of Biotechnology, 5(20): 1924-1928.
  • Gholivand MB, Rahimi-Nasrabadi M, Batooli H, Samimi H, 2012. Chemical composition and antioxidant activity of the essential oil and various extracts of Haplophyllum robustum Bge, Natural Product Research, 26(10): 883-891.
  • Hamdia A, Vianea J, Mahjoub MA, Majouli K, Gad MHH, Kharbach M, Demeyer K, Marzouk Z, Heyden YV, 2018. Polyphenolic contents, antioxidant activities and UPLC–ESI–MSanalysis of Haplophyllum tuberculatum A. Juss leaves extracts. International Journal of Biological Macromolecules, 106: 1071–1079.
  • Henriquez C, Aliaga C, Lissi E, 2004. Kinetics profiles in the reaction of ABTS derived radicals with simple phenols and polyphenols. Journal of the Chilean Chemical Society, 49: 65–67.
  • Karahisar E, Tugay O, Erdoğan Orhan İ, Şenol Deniz FS, Luca SV, Skalicka-Wozniak K, Sahin M, 2019. Metabolite Profiling by Hyphenated Liquid Chromatographic Mass Spectrometric Technique (HPLC-DAD-ESI-Q-TOF-MS/MS) and Neurobiological Potential of Haplophyllum sahinii and H. vulcanicum Extracts. Chemistry & Biodiversity, 16(9): 1-14.
  • Kuete V, Wiench B, Alsaid MS, Alyahya MA, Fankam AG, Shahat AA, Efferth T, 2013. Cytotoxicity, mode of action and antibacterial activities of selected Saudi Arabian medicinal plants. BMC Complementary and Alternative Medicine, 13(1): 354..
  • Mohamed AH, Ali MB, Bashir Ak and Salih AM, 2008. Influence of Haplophyllum tuberculatum on the cardiovascular system. Pharmaceutical Biology, 34(3): 213-217.
  • Napoli E, Siracusa L, Ruberto G, Lazzara ACS, Speciale A, Cimino F, Saija A, Cristani M, 2018. Phytochemical profiles, phototoxic and antioxidant properties of eleven Hypericum species – A comparative study. Phytochemistry, 152: 162-173.
  • Narayanasamy B, Jeyakumar N, Manoharan DK, 2018. Effect of natural antioxidants on the oxidation stability of methyl ester of rubber seed oil. Journal Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 40(6): 680-687.
  • Noipa T, Srijaranai S, Tuntulani T, Ngeontae W, 2011. New approach for evalua-tion of the antioxidant capacity based on scavenging DPPH free radical in micellesystems. Food Research International, 44: 798–806.
  • Orhan IE, Küpeli Akkol E, Suntar I, Yesilada E, 2019. Assessment of anticholinesterase and antioxidant properties of the extracts and (+)-catechin obtained from Arceuthobium oxycedri (D.C.) M. Bieb (dwarf mistletoe). South African Journal of Botany, 120: 309-312.
  • Pasqualone A, Bianco AM, Paradiso VM, Summo C, Gambacorta G, Caponio F. Blanco A, 2015. Production and characterization of functional biscuits obtained from purple wheat. Food Chemistry, 180: 64-70.
  • Raissi A, Arbabi M, Roustakhiz J, Hosseini M, 2016. Haplophyllum tuberculatum: An overview. J. HerbMed Pharmacol, 5(4): 125-130.
  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C, 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26:1231–1237.
  • Sabry OMM, El Sayed AM, Sleem A, 2016b. Potential anti-microbial, anti-inflammatory and anti-oxidant activities of Haplophyllum tuberculatum growing in Libya. Journal of Pharmacognosy and Natural Products, 2:1.
  • Sabry OMM, El Sayed AM, Alshalmani SK, 2016a. GC/MS analysis and potential cytotoxic activity of Haplophyllum tuberculatum essential oils against lung and liver cancer cells. Pharmacognosy Journal, 8(1): 66-69.
  • Shah MA, Don Bosco SJ, Mir SA, 2014. Plant extracts as natural antioxidants in meat and meat products. Meat Science, 98: 21-33.
  • Singleton VL, Orthofer R, Lamuela-Raventós RM, 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. Academic Press, p. 152–178.
  • Skrzypczak A, Przystupa N, Zgadzaj A, Parzonko A, Sykłowska-Baranek K, Paradowska K, Nałęcz-Jaweck G, 2015. Antigenotoxic, anti-photogenotoxic and antioxidant activities of natural naphthoquinone shikonin and acetylshikonin and Arnebia euchroma callus extracts evaluated by the umu-test and EPR method. Toxicology in Vitro, 30(1): 364-372.
  • Snedecor GW, Cochran WG, 1967. Statistical Methods. The Iowa State University.
  • Tlili N, Elfalleh W, Hannachi H, Yahia Y, Khaldi A, Ferchichi A, Nasri N, 2013.Screening of natural antioxidants from selected medicinal plants. International Journal of Food Properties, 16: 1117–1126.
  • Tlili N, Mejri H, Anouer F, Saadaouie E, Khaldie A, Nasri N, 2015. Phenolic profile and antioxidant activity of Capparis spinosa seeds harvested from different wild habitats. Industrial Crops and Products, 76: 930–935.
  • Tlili N, Mejri H, Yahia Y, Saadaoui E, Rejeb S, Khaldi A, Nasri N, 2014. Phyto-chemicals and antioxidant activities of Rhus tripartitum (Ucria) fruits dependingon locality and different stages of maturity. Food Chemistry, 160: 98–103.
  • Townsend CC, 1986. Taxonomic revision of the genus Haplophyllum (Rutaceae). In: Hooker’s icones plantarum vol 40 parts 1, Bentham-Moxon Trustees Royal Botanical Gardens, Kew.
  • Tugay O, Ulukuş D, 2017. Haplophyllum sahinii (Rutaceae), a new species from Central Anatolia (Turkey). Phytotaxa, 297: 265–272.
  • Ulubelen A, Öztürk M, 2008. Alkaloids, coumarins and lignans from Haplophyllum species. Records of Natural Products, 2(3): 54-69.
  • Ulukuş D, Tugay O, 2018. Haplophyllum ermenekense (Rutaceae),a new speciesfromTurkey. Phytokeys, 8(111): 119–131.

The Effect of Location And Species Differences on Antioxidant Activities of Endemic Haplophyllum A. Juss.

Year 2020, Volume: 10 Issue: 1, 648 - 657, 01.03.2020
https://doi.org/10.21597/jist.596057

Abstract

The aim of current study was to determinate antioxidant activities, the total flavonoid and phenolic contents of aerial parts of some endemic Haplophyllum A. Juss. (H. myrtifolium, H. vulcanicum, H. pumiliforme and H. sahinii) collected from different locations of Turkey. The total phenolic content of the samples ranged from 31.8 to 49.8 mg GAEg-1 extract. H. vulcanicum and H. sahinii had higher total phenolic content than other species. The highest total flavonoid amount was detected in the samples of both locations of H. myrtifolium (62.0 and 63.0 mg KE g-1 extract) whereas the lowest was reported in the samples of both locations of H. pumiliforme (36.2 and 31.7 mg KE g-1 extract). Antioxidant activities were measured by radical scavenging activities such as the 2, 2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Location1 samples of H. myrtifolium and location2 samples of H. pumiliforme showed the strongest ABTS radical scavening activity (78.6% and 79.0%, respectively). The samples in two locations of H. vulcanicum had the lowest value for two activities. As a result, H. pumiliforme was found to have low phytochemical content but exhibited high antioxidant activity. The results of antioxidant activities and total bioactive contents about H. vulcanicum, H. sahini and H. pumiliforme is reported by us for the first time.

Project Number

13201023

References

  • Al-Rehaily AJ, Alqasoumi SI, Yusufoglu HS, Al-Yahya MA, Demirci B, Tabanca N, Wedge DE, Demirci F, Bernier UR, Becnel JJ, Temel HE, Can Baser KH, 2014. Chemical composition and biological activity of Haplophyllum tuberculatum Juss essential oil. Journal of Essential Oil Bearing Plants, 17(3): 452-459.
  • Arvouet-Grand A, Vennat B, Pourrat A, Legret P, 1994. Standardization of a propolis extract and identification of the main constituents. Journal de pharmacie de Belgique, 49: 462–468.
  • Ashraf MA, Iqbal M, Rasheed R, Hussain I, Riaz M, Arif MS, 2018. Chapter 8 - Environmental Stress and Secondary Metabolites in Plants: An Overview. Plant Metabolites and Regulation Under Environmental Stress, 153-167.
  • Awaad AS, Alothman, EAA, 2018. Antiulcer and Anti-ulcerative Colitis Activities of Haplophyllum tuberculatum (Forsskal). International Journal of Pharmacology, 14(1): 31-38.
  • Bajaj S, Urooj A, Prabhasankar P, 2006. Effect of incorporation of mint on texture, colour and sensory parameters of biscuits. International Journal of Food Properties, 9: 691-700.
  • Ben Farhat M, Landoulsi A, Chaouch-Hamada R, Sotomayor JA, Jordánc MJ, 2013. Characterization and quantification of phenolic compounds and antioxi-dant properties of Salvia species growing in different habitats. Industrial Crops and Products, 49: 904–914.
  • Deng B, Fang S, Shang X, Fu X, Yang W, 2019. Influence of genotypes and environmental factors on leaf triterpenoid content and growth of Cyclocarya paliurus. Journal of Forestry Research, 30(3): 789–798.
  • Debouba M, Khemakhem B, Zouari S, Meskine A, Gouia H, 2014. Chemical and Biological Activities of Haplophyllumtuberculatum Organic Extracts and Essential Oil. Journal of Essential Oil Bearing Plants, 17(5): 787-796.
  • Düzgüneş O, Kesici T, Gürbüz F, 1983. İstatistik Metotları 1. Anakara Üniversitesi Ziraat Fakültesi Yay. No: 862, Ankara.
  • Eissa T, González-Burgos E, Carretero M, Gómez-Serranillos M, 2014. Biologicalactivity of HPLC-characterized ethanol extract from the aerial parts of Haplophyllum tuberculatum, Pharmaceutical Biology, 52(2): 151–156.
  • Gezer K, Duru ME, Kivrak I, Turkoglu A, Mercan N, Turkoglu H, Gülcan S, 2006. Free-radical scavenging capacity and antimicrobial activity of wild edible mushroom from Turkey. African Journal of Biotechnology, 5(20): 1924-1928.
  • Gholivand MB, Rahimi-Nasrabadi M, Batooli H, Samimi H, 2012. Chemical composition and antioxidant activity of the essential oil and various extracts of Haplophyllum robustum Bge, Natural Product Research, 26(10): 883-891.
  • Hamdia A, Vianea J, Mahjoub MA, Majouli K, Gad MHH, Kharbach M, Demeyer K, Marzouk Z, Heyden YV, 2018. Polyphenolic contents, antioxidant activities and UPLC–ESI–MSanalysis of Haplophyllum tuberculatum A. Juss leaves extracts. International Journal of Biological Macromolecules, 106: 1071–1079.
  • Henriquez C, Aliaga C, Lissi E, 2004. Kinetics profiles in the reaction of ABTS derived radicals with simple phenols and polyphenols. Journal of the Chilean Chemical Society, 49: 65–67.
  • Karahisar E, Tugay O, Erdoğan Orhan İ, Şenol Deniz FS, Luca SV, Skalicka-Wozniak K, Sahin M, 2019. Metabolite Profiling by Hyphenated Liquid Chromatographic Mass Spectrometric Technique (HPLC-DAD-ESI-Q-TOF-MS/MS) and Neurobiological Potential of Haplophyllum sahinii and H. vulcanicum Extracts. Chemistry & Biodiversity, 16(9): 1-14.
  • Kuete V, Wiench B, Alsaid MS, Alyahya MA, Fankam AG, Shahat AA, Efferth T, 2013. Cytotoxicity, mode of action and antibacterial activities of selected Saudi Arabian medicinal plants. BMC Complementary and Alternative Medicine, 13(1): 354..
  • Mohamed AH, Ali MB, Bashir Ak and Salih AM, 2008. Influence of Haplophyllum tuberculatum on the cardiovascular system. Pharmaceutical Biology, 34(3): 213-217.
  • Napoli E, Siracusa L, Ruberto G, Lazzara ACS, Speciale A, Cimino F, Saija A, Cristani M, 2018. Phytochemical profiles, phototoxic and antioxidant properties of eleven Hypericum species – A comparative study. Phytochemistry, 152: 162-173.
  • Narayanasamy B, Jeyakumar N, Manoharan DK, 2018. Effect of natural antioxidants on the oxidation stability of methyl ester of rubber seed oil. Journal Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 40(6): 680-687.
  • Noipa T, Srijaranai S, Tuntulani T, Ngeontae W, 2011. New approach for evalua-tion of the antioxidant capacity based on scavenging DPPH free radical in micellesystems. Food Research International, 44: 798–806.
  • Orhan IE, Küpeli Akkol E, Suntar I, Yesilada E, 2019. Assessment of anticholinesterase and antioxidant properties of the extracts and (+)-catechin obtained from Arceuthobium oxycedri (D.C.) M. Bieb (dwarf mistletoe). South African Journal of Botany, 120: 309-312.
  • Pasqualone A, Bianco AM, Paradiso VM, Summo C, Gambacorta G, Caponio F. Blanco A, 2015. Production and characterization of functional biscuits obtained from purple wheat. Food Chemistry, 180: 64-70.
  • Raissi A, Arbabi M, Roustakhiz J, Hosseini M, 2016. Haplophyllum tuberculatum: An overview. J. HerbMed Pharmacol, 5(4): 125-130.
  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C, 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology & Medicine, 26:1231–1237.
  • Sabry OMM, El Sayed AM, Sleem A, 2016b. Potential anti-microbial, anti-inflammatory and anti-oxidant activities of Haplophyllum tuberculatum growing in Libya. Journal of Pharmacognosy and Natural Products, 2:1.
  • Sabry OMM, El Sayed AM, Alshalmani SK, 2016a. GC/MS analysis and potential cytotoxic activity of Haplophyllum tuberculatum essential oils against lung and liver cancer cells. Pharmacognosy Journal, 8(1): 66-69.
  • Shah MA, Don Bosco SJ, Mir SA, 2014. Plant extracts as natural antioxidants in meat and meat products. Meat Science, 98: 21-33.
  • Singleton VL, Orthofer R, Lamuela-Raventós RM, 1999. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. Academic Press, p. 152–178.
  • Skrzypczak A, Przystupa N, Zgadzaj A, Parzonko A, Sykłowska-Baranek K, Paradowska K, Nałęcz-Jaweck G, 2015. Antigenotoxic, anti-photogenotoxic and antioxidant activities of natural naphthoquinone shikonin and acetylshikonin and Arnebia euchroma callus extracts evaluated by the umu-test and EPR method. Toxicology in Vitro, 30(1): 364-372.
  • Snedecor GW, Cochran WG, 1967. Statistical Methods. The Iowa State University.
  • Tlili N, Elfalleh W, Hannachi H, Yahia Y, Khaldi A, Ferchichi A, Nasri N, 2013.Screening of natural antioxidants from selected medicinal plants. International Journal of Food Properties, 16: 1117–1126.
  • Tlili N, Mejri H, Anouer F, Saadaouie E, Khaldie A, Nasri N, 2015. Phenolic profile and antioxidant activity of Capparis spinosa seeds harvested from different wild habitats. Industrial Crops and Products, 76: 930–935.
  • Tlili N, Mejri H, Yahia Y, Saadaoui E, Rejeb S, Khaldi A, Nasri N, 2014. Phyto-chemicals and antioxidant activities of Rhus tripartitum (Ucria) fruits dependingon locality and different stages of maturity. Food Chemistry, 160: 98–103.
  • Townsend CC, 1986. Taxonomic revision of the genus Haplophyllum (Rutaceae). In: Hooker’s icones plantarum vol 40 parts 1, Bentham-Moxon Trustees Royal Botanical Gardens, Kew.
  • Tugay O, Ulukuş D, 2017. Haplophyllum sahinii (Rutaceae), a new species from Central Anatolia (Turkey). Phytotaxa, 297: 265–272.
  • Ulubelen A, Öztürk M, 2008. Alkaloids, coumarins and lignans from Haplophyllum species. Records of Natural Products, 2(3): 54-69.
  • Ulukuş D, Tugay O, 2018. Haplophyllum ermenekense (Rutaceae),a new speciesfromTurkey. Phytokeys, 8(111): 119–131.
There are 37 citations in total.

Details

Primary Language Turkish
Subjects Agronomy
Journal Section Tarla Bitkileri / Field Crops
Authors

Cennet Yaman 0000-0002-2364-8171

Osman Tugay 0000-0003-3980-7648

Deniz Ulukuş 0000-0002-9627-5492

Project Number 13201023
Publication Date March 1, 2020
Submission Date July 24, 2019
Acceptance Date November 21, 2019
Published in Issue Year 2020 Volume: 10 Issue: 1

Cite

APA Yaman, C., Tugay, O., & Ulukuş, D. (2020). Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon ve Tür Farkının Etkisi. Journal of the Institute of Science and Technology, 10(1), 648-657. https://doi.org/10.21597/jist.596057
AMA Yaman C, Tugay O, Ulukuş D. Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon ve Tür Farkının Etkisi. J. Inst. Sci. and Tech. March 2020;10(1):648-657. doi:10.21597/jist.596057
Chicago Yaman, Cennet, Osman Tugay, and Deniz Ulukuş. “Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon Ve Tür Farkının Etkisi”. Journal of the Institute of Science and Technology 10, no. 1 (March 2020): 648-57. https://doi.org/10.21597/jist.596057.
EndNote Yaman C, Tugay O, Ulukuş D (March 1, 2020) Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon ve Tür Farkının Etkisi. Journal of the Institute of Science and Technology 10 1 648–657.
IEEE C. Yaman, O. Tugay, and D. Ulukuş, “Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon ve Tür Farkının Etkisi”, J. Inst. Sci. and Tech., vol. 10, no. 1, pp. 648–657, 2020, doi: 10.21597/jist.596057.
ISNAD Yaman, Cennet et al. “Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon Ve Tür Farkının Etkisi”. Journal of the Institute of Science and Technology 10/1 (March 2020), 648-657. https://doi.org/10.21597/jist.596057.
JAMA Yaman C, Tugay O, Ulukuş D. Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon ve Tür Farkının Etkisi. J. Inst. Sci. and Tech. 2020;10:648–657.
MLA Yaman, Cennet et al. “Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon Ve Tür Farkının Etkisi”. Journal of the Institute of Science and Technology, vol. 10, no. 1, 2020, pp. 648-57, doi:10.21597/jist.596057.
Vancouver Yaman C, Tugay O, Ulukuş D. Endemik Haplophyllum A. Juss. Türlerinin Antioksidan Aktivitesi Üzerine Lokasyon ve Tür Farkının Etkisi. J. Inst. Sci. and Tech. 2020;10(1):648-57.