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Nitric Oxide Molecule as A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows

Year 2020, Volume: 10 Issue: 4, 2740 - 2746, 15.12.2020

Canan Gülmez , Onur Atakisi

https://doi.org/10.21597/jist.737379

Abstract

The mastitis is an important disease affecting daily milk yield and quality in lactating cows, it negatively affects both animal welfare and economic income. The aim of this study is to investigate the effect of subclinical mastitis on nitric oxide (NO), total antioxidant capacity (TAC), total oxidant capacity (TOC), albumin, total protein, globulin, glucose and gamma glutamyl transpeptidase activity (GGT) levels in plasma. The research was carried out in Brown Swiss and Holstein cows. The mammary lobes of the animals were scanned with the California Mastitis Test (CMT) and those with subclinical mastitis were identified. Samples with CMT (-) were classified as healthy (n = 10) and those with CMT (+) were classified as subclinical mastitis group (n = 10). The study presented is a basic feature and results clearly showed that significant amounts of NO was released in plasma during subclinical mastitis. There was no statistically significant difference between cows with or without subclinical mastitis for plasma TAC, TOC, albumin, total protein, globulin, glucose, albumin/globulin levels and GGT activity. Consequently, nitric oxide is an important mediator in subclinical mastitis as a potential target of therapeutic strategies and can be evaluated in alternative diagnosis.

Keywords

Subclinical mastitis Cow Nitric oxide

References

  • Alba DF, da Rosa G, Hanauer D, Saldanha TF, Souza CF, Baldissera MD, Da Silva AS, 2019. Subclinical mastitis in Lacaune sheep: Causative agents, impacts on milk production, milk quality, oxidative profiles and treatment efficacy of ceftiofur. Microbial Pathogenesis, 137: 103732.
  • Atakisi O, Oral H, Atakisi E, Merhan O, Pancarci SM, Ozcan A, Marasli S, Polat B, Colak A, Kaya S, 2010. Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Research in Veterinary Science, 89(1): 10-13.
  • Basu S, Eriksson M, 2001. Retinol palmitate counteracts oxidative injury during experimental septic shock. Annals of the Academy of Medicine, 30: 265–269.
  • Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA, 1990. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proceedings of the National Academy of Sciences, 87: 1620–24.
  • Bhosale RR, Osmani RA, Ghodake PP, Shaikh SM, Chavan SR, 2014. Mastitis: an intensive crisis in veterinary science. International Journal of Pharma Research and Health Sciences, 2: 96–103.
  • Bouchard L, Blais S, Desrosiers C, Zhao X, Lacasse P,1999. Nitric oxide production during endotoxin-induced mastitis in the cow. Journal of Dairy Science, 82(12): 2574-2581.
  • Brito M, González FD, Ribeiro LA, Campos R., Lacerda, L, Barbosa PR, Bergmann, G, 2006. Composição do sangue e do leite em ovinos leiteiros do sul do Brasil: variações na gestação e na lactação. Ciência Rural, 36 (3): 942–948.
  • Cardozo VF, Lancheros CA, Narciso AM, Valereto EC, Kobayashi RK, Seabra AB, Nakazato, G, 2014. Evaluation of antibacterial activity of nitric oxide-releasing polymeric particles against Staphylococcus aureus and Escherichia coli from bovine mastitis. International Journal of Pharmaceutics, 473(1-2): 20-29.
  • Contreras-Aguilar MD, Monkeviciene I, Ceron JJ, Silinskas I, Vallejo-Mateo PJ, Tecles F, Zelvyte R, 2019. Biochemical changes in saliva of cows with inflammation: A pilot study. Research in Veterinary Science, 124: 383-386.
  • Conzuelo F, Campuzano S, Gamella M, Pinacho DG, Reviejo AJ, Marco MP, Pingarrón JM, 2013. Integrated disposable electrochemical immunosensors for the simultaneous determination of sulfonamide and tetracycline antibiotics residues in milk. Biosensensors and Bioelectronics, 50: 100–105.
  • Cortinhas CS, Tomazi T, Zoni MSF, Moro E, Santos, MV, 2016 Randomized clinical trial comparing ceftiofur hydrochloride with a positive control protocol for intramammary treatment of nonsevere clinical mastitis in dairy cows. Jounal Dairy Science, 99 (7): 5619–5628.
  • Cristina M, Melo RT, Mendonça EP, Coelho LR, Rossi DA, 2010. Uso de ceftiofur em vacas leiteiras e sua excreção no leite, PUBVET. Londrina 4 (33): 931–937.
  • Enger BD, 2019. Invited Review: Reevaluating how mastitis reduces milk yield: Discussion of competitive substrate utilization. Applied Animal Science, 35(4): 408-415.
  • Erel O, 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38: 1103–1111.
  • Giannini EG, Testa R, Savarino V, 2005. Liver enzyme alteration: a guide for clinicians. Canadian Medical Association Journal, 172(3): 367-379.
  • Ilgun T, Dalgınlı KY, Gulmez C, Atakisi O, 2016. Changes in the levels of liver HSP70, plasma nitric oxide and antioxidative system in experimentally induced endotoxemia mouse model and the role of reduced glutathione. Turkish Journal of Biology, 40: 1272-1277.
  • Ingman WV, Glynn DJ, Hutchinson MR, 2014. Inflammatory mediators in mastitis and lactation insufficiency. Journal of Mammary Gland Biology and Neoplasia, 19(2):161-167.
  • Lee M, Rey K, Besler K, Wang C, Choy J, 2017. Immunobiology of nitric oxide and regulation of inducible nitric oxide synthase. Macrophages, 62: 181-207
  • Lykkesfeldt J, Svendsen O, 2007. Oxidants and antioxidants in disease: oxidative stress in farm animals. Veterinary Journal, 173: 502–511.
  • Miranda KM, Espey MG, Wink DA, 2001. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide: Biology and Chemistry, 5: 62–71.
  • Nedić S, Vakanjac S, Samardžija M, Borozan S, 2019. Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus. Research in Veterinary Science, 125: 323-332.
  • Neto AE, Júnior FG, Santos JCA, Silva LCA, Matos RAT, 2015. Evaluation of antibiotic residues in milk samples from cows after dry cow therapy. Arquivos do Instituto Biológico, 82: 1–4.
  • Osman KM, Hassan HM, Ibrahim IM, Mikhail MM, 2010. The impact of staphylococcal mastitis on the level of milk IL-6, lysozyme and nitric oxide. Comparative immunology, microbiology and infectious diseases, 33(1): 85-93.
  • Piotrowska-Tomala KK, Bah MM, Jankowska K, Lukasik K, Warmowski P, Galvao AM, Skarzynski DJ, 2015. Lipopolysaccharides, cytokines, and nitric oxide affect secretion of prostaglandins and leukotrienes by bovine mammary gland during experimentally induced mastitis in vivo and in vitro. Domestic Animal Endocrinology, 52:90-99.
  • Pryor WA, Squadrito GL, 1995. The chemistry of peroxynitrite: a product from the reaction of nitric oxide with superoxide. American Journal of Physiology, 268: 699–722.
  • Regev G, Martins J, Sheridan MP, Leemhuis J, Thompson J, Miller C, 2018. Feasibility and preliminary safety of nitric oxide releasing solution as a treatment for bovine mastitis. Research in Veterinary Science, 118: 247-253.
  • Seegers H, Fourichon C, Beaudeau F, 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research. 34: 475–491.
  • Silanikove N, Merin U, Shapiro F, Leitner G, 2014. Subclinical mastitis in goats is associated with upregulation of nitric oxide-derived oxidative stress that causes reduction of milk antioxidative properties and impairment of its quality. Journal of Dairy Science, 97(6): 3449-3455.
  • Simontacchi M, Garcia-Mata C, Bartoli Cg, Santa-Maria Ge, Lamattina L, 2013. Nitric oxide as a key component in hormone-regulated processes. Plant Cell Reports, 32(6): 853-866.
  • Tothova CS, Nagy O, Kovac G, 2014. Acute phase proteins and their use in the diagnosis of diseases in ruminants: a review. Veterinarni Medicina, 59(4).
  • Turk R, Piras C, Kovačić M, Samardžija M, Ahmed H, De Canio M, ... Roncada P, 2012. Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis. Journal of Proteomics, 75(14): 4412-4428.
  • Zduńczyk S. Janowski T, 2020. Bacteriophages and associated endolysins in therapy and prevention of mastitis and metritis in cows: Current knowledge. Animal Reproduction Science, 106504.

Nitric Oxide Molecule as A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows

Year 2020, Volume: 10 Issue: 4, 2740 - 2746, 15.12.2020

Canan Gülmez , Onur Atakisi

https://doi.org/10.21597/jist.737379

Abstract

The mastitis is an important disease affecting daily milk yield and quality in lactating cows, it negatively affects both animal welfare and economic income. The aim of this study is to investigate the effect of subclinical mastitis on nitric oxide (NO), total antioxidant capacity (TAC), total oxidant capacity (TOC), albumin, total protein, globulin, glucose and gamma glutamyl transpeptidase activity (GGT) levels in plasma. The research was carried out in Brown Swiss and Holstein cows. The mammary lobes of the animals were scanned with the California Mastitis Test (CMT) and those with subclinical mastitis were identified. Samples with CMT (-) were classified as healthy (n = 10) and those with CMT (+) were classified as subclinical mastitis group (n = 10). The study presented is a basic feature and results clearly showed that significant amounts of NO was released in plasma during subclinical mastitis. There was no statistically significant difference between cows with or without subclinical mastitis for plasma TAC, TOC, albumin, total protein, globulin, glucose, albumin/globulin levels and GGT activity. Consequently, nitric oxide is an important mediator in subclinical mastitis as a potential target of therapeutic strategies and can be evaluated in alternative diagnosis.

Keywords

Subclinical mastitis Cow Nitric oxide

References

  • Alba DF, da Rosa G, Hanauer D, Saldanha TF, Souza CF, Baldissera MD, Da Silva AS, 2019. Subclinical mastitis in Lacaune sheep: Causative agents, impacts on milk production, milk quality, oxidative profiles and treatment efficacy of ceftiofur. Microbial Pathogenesis, 137: 103732.
  • Atakisi O, Oral H, Atakisi E, Merhan O, Pancarci SM, Ozcan A, Marasli S, Polat B, Colak A, Kaya S, 2010. Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk. Research in Veterinary Science, 89(1): 10-13.
  • Basu S, Eriksson M, 2001. Retinol palmitate counteracts oxidative injury during experimental septic shock. Annals of the Academy of Medicine, 30: 265–269.
  • Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA, 1990. Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide. Proceedings of the National Academy of Sciences, 87: 1620–24.
  • Bhosale RR, Osmani RA, Ghodake PP, Shaikh SM, Chavan SR, 2014. Mastitis: an intensive crisis in veterinary science. International Journal of Pharma Research and Health Sciences, 2: 96–103.
  • Bouchard L, Blais S, Desrosiers C, Zhao X, Lacasse P,1999. Nitric oxide production during endotoxin-induced mastitis in the cow. Journal of Dairy Science, 82(12): 2574-2581.
  • Brito M, González FD, Ribeiro LA, Campos R., Lacerda, L, Barbosa PR, Bergmann, G, 2006. Composição do sangue e do leite em ovinos leiteiros do sul do Brasil: variações na gestação e na lactação. Ciência Rural, 36 (3): 942–948.
  • Cardozo VF, Lancheros CA, Narciso AM, Valereto EC, Kobayashi RK, Seabra AB, Nakazato, G, 2014. Evaluation of antibacterial activity of nitric oxide-releasing polymeric particles against Staphylococcus aureus and Escherichia coli from bovine mastitis. International Journal of Pharmaceutics, 473(1-2): 20-29.
  • Contreras-Aguilar MD, Monkeviciene I, Ceron JJ, Silinskas I, Vallejo-Mateo PJ, Tecles F, Zelvyte R, 2019. Biochemical changes in saliva of cows with inflammation: A pilot study. Research in Veterinary Science, 124: 383-386.
  • Conzuelo F, Campuzano S, Gamella M, Pinacho DG, Reviejo AJ, Marco MP, Pingarrón JM, 2013. Integrated disposable electrochemical immunosensors for the simultaneous determination of sulfonamide and tetracycline antibiotics residues in milk. Biosensensors and Bioelectronics, 50: 100–105.
  • Cortinhas CS, Tomazi T, Zoni MSF, Moro E, Santos, MV, 2016 Randomized clinical trial comparing ceftiofur hydrochloride with a positive control protocol for intramammary treatment of nonsevere clinical mastitis in dairy cows. Jounal Dairy Science, 99 (7): 5619–5628.
  • Cristina M, Melo RT, Mendonça EP, Coelho LR, Rossi DA, 2010. Uso de ceftiofur em vacas leiteiras e sua excreção no leite, PUBVET. Londrina 4 (33): 931–937.
  • Enger BD, 2019. Invited Review: Reevaluating how mastitis reduces milk yield: Discussion of competitive substrate utilization. Applied Animal Science, 35(4): 408-415.
  • Erel O, 2005. A new automated colorimetric method for measuring total oxidant status. Clinical Biochemistry, 38: 1103–1111.
  • Giannini EG, Testa R, Savarino V, 2005. Liver enzyme alteration: a guide for clinicians. Canadian Medical Association Journal, 172(3): 367-379.
  • Ilgun T, Dalgınlı KY, Gulmez C, Atakisi O, 2016. Changes in the levels of liver HSP70, plasma nitric oxide and antioxidative system in experimentally induced endotoxemia mouse model and the role of reduced glutathione. Turkish Journal of Biology, 40: 1272-1277.
  • Ingman WV, Glynn DJ, Hutchinson MR, 2014. Inflammatory mediators in mastitis and lactation insufficiency. Journal of Mammary Gland Biology and Neoplasia, 19(2):161-167.
  • Lee M, Rey K, Besler K, Wang C, Choy J, 2017. Immunobiology of nitric oxide and regulation of inducible nitric oxide synthase. Macrophages, 62: 181-207
  • Lykkesfeldt J, Svendsen O, 2007. Oxidants and antioxidants in disease: oxidative stress in farm animals. Veterinary Journal, 173: 502–511.
  • Miranda KM, Espey MG, Wink DA, 2001. A rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide: Biology and Chemistry, 5: 62–71.
  • Nedić S, Vakanjac S, Samardžija M, Borozan S, 2019. Paraoxonase 1 in bovine milk and blood as marker of subclinical mastitis caused by Staphylococcus aureus. Research in Veterinary Science, 125: 323-332.
  • Neto AE, Júnior FG, Santos JCA, Silva LCA, Matos RAT, 2015. Evaluation of antibiotic residues in milk samples from cows after dry cow therapy. Arquivos do Instituto Biológico, 82: 1–4.
  • Osman KM, Hassan HM, Ibrahim IM, Mikhail MM, 2010. The impact of staphylococcal mastitis on the level of milk IL-6, lysozyme and nitric oxide. Comparative immunology, microbiology and infectious diseases, 33(1): 85-93.
  • Piotrowska-Tomala KK, Bah MM, Jankowska K, Lukasik K, Warmowski P, Galvao AM, Skarzynski DJ, 2015. Lipopolysaccharides, cytokines, and nitric oxide affect secretion of prostaglandins and leukotrienes by bovine mammary gland during experimentally induced mastitis in vivo and in vitro. Domestic Animal Endocrinology, 52:90-99.
  • Pryor WA, Squadrito GL, 1995. The chemistry of peroxynitrite: a product from the reaction of nitric oxide with superoxide. American Journal of Physiology, 268: 699–722.
  • Regev G, Martins J, Sheridan MP, Leemhuis J, Thompson J, Miller C, 2018. Feasibility and preliminary safety of nitric oxide releasing solution as a treatment for bovine mastitis. Research in Veterinary Science, 118: 247-253.
  • Seegers H, Fourichon C, Beaudeau F, 2003. Production effects related to mastitis and mastitis economics in dairy cattle herds. Veterinary Research. 34: 475–491.
  • Silanikove N, Merin U, Shapiro F, Leitner G, 2014. Subclinical mastitis in goats is associated with upregulation of nitric oxide-derived oxidative stress that causes reduction of milk antioxidative properties and impairment of its quality. Journal of Dairy Science, 97(6): 3449-3455.
  • Simontacchi M, Garcia-Mata C, Bartoli Cg, Santa-Maria Ge, Lamattina L, 2013. Nitric oxide as a key component in hormone-regulated processes. Plant Cell Reports, 32(6): 853-866.
  • Tothova CS, Nagy O, Kovac G, 2014. Acute phase proteins and their use in the diagnosis of diseases in ruminants: a review. Veterinarni Medicina, 59(4).
  • Turk R, Piras C, Kovačić M, Samardžija M, Ahmed H, De Canio M, ... Roncada P, 2012. Proteomics of inflammatory and oxidative stress response in cows with subclinical and clinical mastitis. Journal of Proteomics, 75(14): 4412-4428.
  • Zduńczyk S. Janowski T, 2020. Bacteriophages and associated endolysins in therapy and prevention of mastitis and metritis in cows: Current knowledge. Animal Reproduction Science, 106504.
There are 32 citations in total.

Details

Primary Language English
Subjects Structural Biology
Journal Section Kimya / Chemistry
Authors

Canan Gülmez 0000-0003-3253-1407

Onur Atakisi 0000-0003-1183-6076

Publication Date December 15, 2020
Submission Date May 14, 2020
Acceptance Date July 27, 2020
Published in Issue Year 2020 Volume: 10 Issue: 4

Cite

APA Gülmez, C., & Atakisi, O. (2020). Nitric Oxide Molecule as A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows. Journal of the Institute of Science and Technology, 10(4), 2740-2746. https://doi.org/10.21597/jist.737379
AMA Gülmez C, Atakisi O. Nitric Oxide Molecule as A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows. J. Inst. Sci. and Tech. December 2020;10(4):2740-2746. doi:10.21597/jist.737379
Chicago Gülmez, Canan, and Onur Atakisi. “Nitric Oxide Molecule As A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows”. Journal of the Institute of Science and Technology 10, no. 4 (December 2020): 2740-46. https://doi.org/10.21597/jist.737379.
EndNote Gülmez C, Atakisi O (December 1, 2020) Nitric Oxide Molecule as A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows. Journal of the Institute of Science and Technology 10 4 2740–2746.
IEEE C. Gülmez and O. Atakisi, “Nitric Oxide Molecule as A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows”, J. Inst. Sci. and Tech., vol. 10, no. 4, pp. 2740–2746, 2020, doi: 10.21597/jist.737379.
ISNAD Gülmez, Canan - Atakisi, Onur. “Nitric Oxide Molecule As A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows”. Journal of the Institute of Science and Technology 10/4 (December 2020), 2740-2746. https://doi.org/10.21597/jist.737379.
JAMA Gülmez C, Atakisi O. Nitric Oxide Molecule as A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows. J. Inst. Sci. and Tech. 2020;10:2740–2746.
MLA Gülmez, Canan and Onur Atakisi. “Nitric Oxide Molecule As A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows”. Journal of the Institute of Science and Technology, vol. 10, no. 4, 2020, pp. 2740-6, doi:10.21597/jist.737379.
Vancouver Gülmez C, Atakisi O. Nitric Oxide Molecule as A Potential Target of Alternative Strategies for the Diagnosis of Subclinical Mastitis in Cows. J. Inst. Sci. and Tech. 2020;10(4):2740-6.