Hypericum L. Cinsine Ait Bazı Türlerin Uçucu Yağlarının Antibakteriyel Etkisi

Year 2023, Volume: 13 Issue: 1, 73 - 79, 01.03.2023

Altuğ Karaman , Neslihan Taşar , Ferit Can Yazdıç , Osman Gedik

https://doi.org/10.21597/jist.1136935

Abstract

Bitkilerden elde edilen biyolojik aktif bileşenlerin incelenmesi son zamanlarda en çok araştırılan
konular arasındadır. Türkiye florasının çok zengin bir yapıya sahip olması, bu coğrafyada yetişen
bitkilerin araştırılmasının önemli olduğunu göstermektedir. Bu çalışmada, kimyasal bileşen
olarak eski ve yaygın bir kullanım alanına sahip olan aynı zamanda önemli bir tıbbi bitki olan
Hypericum cinsine ait 3 taksondan elde edilen uçucu yağlar antibakteriyel etkileri yönüyle
araştırılmıştır. Hypericum spectabile Jaub. & Spach (endemik tür), H. scabrum L., H. venustum
Fenzl. türlerinin uçucu yağları distilasyon yöntemi kullanılarak elde edilmiştir. Elde edilen bu
yağların antibakteriyel etkileri, in vitro olarak birçok ilaca direnç gösterdiği bilinen; Enterobacter
aerogenes (ATCC 13048), Enterococcus faecalis (ATCC 29212), Salmonella enteritidis (ATCC
13075), Staphylococcus aureus subsp. aureus (ATCC 25923), Escherichia coli (ATCC 25922)
ve Serratia marcescens (ATCC 13880) patojen bakterileri kullanılarak araştırılmıştır. Türlerin
farklı uçucu yağları, çeşitli mikroorganizmalara karşı antibakteriyel aktivite için oyuk agar testi
kullanılarak analiz edilmiştir. Tüm uçucu yağların test edilen tüm patojen strainlerine karşı
değişen oranlarda (4,80±0,87-27,73±1,27 mm) aktivite gösterdiği gözlenmiştir. Bu türler,
literatürde antibakteriyel aktivite açısından yeni değerlendirilen türler arasında gösterilebilir.

Keywords

endemik, hypericum, uçucu yağ, antibakteriyel

Antibacterial Effect of Essential Oil Components of Some Species Belonging to the Genus Hypericum L.

Abstract

Examination of biologically active compounds obtained from plants is among the most
researched subjects recently. The fact that the flora of Turkey has a very rich structure shows that
it is important to research the plants that grow in this geography. In this study, essential oils
obtained from 3 taxa of Hypericum genus, which has an old and widespread use as a chemical
component and an important medicinal plant, were investigated in terms of their antibacterial
effects. Hypericum spectabile Jaub. & Spach (endemic species), Hypericum scabrum L.,
Hypericum venustum Fenzl. the essential oils of the species were obtained by using the
distillation method. The antibacterial effects of these obtained oils are known to be resistant to
many drugs in vitro; Enterobacter aerogenes (ATCC 13048), Enterococcus faecalis (ATCC
29212), Salmonella enteritidis (ATCC 13075), Staphylococcus aureus subsp. aureus (ATCC
25923), Escherichia coli (ATCC 25922) and Serratia marcescens (ATCC 13880) pathogenic
bacteria. Different extraction extracts of the species were analyzed using the well agar test for
antibacterial activity against various microorganisms. It was observed that all extracts showed
varying rates (4.80±0.87-27.73±1.27 mm) activity against all tested pathogen strains. These
species can be shown among the newly evaluated species in the literature in terms of antibacterial
activity.

Keywords

endemic, hypericum, essential oil, antibacterial

References

• Amiri, M. S., Jabbarzadeh, P., & Akhondi, M. (2012). An ethnobotanical survey of medicinal plants used by indigenous people in Zangelanlo district, Northeast Iran. J Med Plants Res, 6(5), 749-753.
• Azhdarzadeh, F., & Hojjati, M. (2016). Chemical composition and antimicrobial activity of leaf, ripe and unripe peel of bitter orange (Citrus aurantium) essential oils. Nutrition and Food Sciences Research, 3(1), 43-50.
• Bajpai, V. K., Baek, K. H., & Kang, S. C. (2012). Control of Salmonella in foods by using essential oils: A review. Food Research International, 45(2), 722-734.
• Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of pharmaceutical analysis, 6(2), 71-79.
• Barbagallo, C., & Chisari, G. (1987). Antimicrobial activity of three Hypericum species. Fitoterapia, 58(3), 175-177.
• Baytop T. (1983). Therapy with medicinal plants in Turkey, Istanbul University Press, Istanbul, pp.197.
• Cavaliere, C., Rea, P., Lynch, M. E., & Blumenthal, M. (2009). Herbal supplement sales experience slight increase in 2008. HerbalGram.
• Chouhan, S., Sharma, K., & Guleria, S. (2017). Antimicrobial activity of some essential oils—present status and future perspectives. Medicines, 4(3), 58.
• Cox, S. D., Mann, C. M., Markham, J. L., Bell, H. C., Gustafson, J. E., Warmington, J. R., & Wyllie, S. G. (2000). The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). Journal of applied microbiology, 88(1), 170-175.
• Cox, S. D., Mann, C. M., Markham, J. L., Gustafson, J. E., Warmington, J. R., & Wyllie, S. G. (2001). Determining the antimicrobial actions of tea tree oil. Molecules, 6(2), 87-91.
• Davis P.H. (1967). Flora of Turkey. Volume II. University of Edinburg, Edinburg.
• Diao, W. R., Hu, Q. P., Zhang, H., & Xu, J. G. (2014). Chemical composition, antibacterial activity and mechanism of action of essential oil from seeds of fennel (Foeniculum vulgare Mill.). Food control, 35(1), 109-116.
• Dobrindt, U., Hentschel, U., Kaper, J. B., & Hacker, J. (2002). Genome plasticity in pathogenic and nonpathogenic enterobacteria. Pathogenicity Islands and the Evolution of Pathogenic Microbes: Volume I, 157-175.
• Dorman, H. D., & Deans, S. G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology, 88(2), 308-316.
• Duval-Iflah, Y., Raibaud, P., Tancrede, C., & Rousseau, M. (1980). R-plasmic transfer from Serratia liquefaciens to Escherichia coli in vitro and in vivo in the digestive tract of gnotobiotic mice associated with human fecal flora. Infection and Immunity, 28(3), 981-990.
• Ebrahimzadeh, M. A., Nabavi, S. M., Nabavi, S. F., & Ahangar, N. (2013). Anticonvulsant activity of Hypericum scabrum L.; possible mechanism involved. Eur Rev Med Pharmacol Sci, 17(16), 2141-4.
• Eslami, B., Nabavi, S. F., Nabavi, S. M., Ebrahimzadeh, M. A., & Mahmoudi, M. (2011). Pharmacological activities of Hypericum scabrum L. Eur Rev Med Pharmacol Sci, 15(5), 32-37.
• Fadli, M., Saad, A., Sayadi, S., Chevalier, J., Mezrioui, N. E., Pagès, J. M., & Hassani, L. (2012). Antibacterial activity of Thymus maroccanus and Thymus broussonetii essential oils against nosocomial infection–bacteria and their synergistic potential with antibiotics. Phytomedicine, 19(5), 464-471.
• Fahed, L., Beyrouthy, M. E., Ouaini, N., Eparvier, V., Stien, D., Vitalini, S., & Iriti, M. (2021). Antimicrobial activity and synergy investigation of Hypericum scabrum essential oil with antifungal drugs. Molecules, 26(21), 6545.
• Fakir, H., Korkmaz, M., & Güller, B. (2009). Medicinal plant diversity of western Mediterrenean region in Turkey. Journal of Applied Biological Sciences, 3(2), 33-43.
• Fischbach, M. A. (2011). Combination therapies for combating antimicrobial resistance. Current opinion in microbiology, 14(5), 519-523.
• Gu, K., Song, Z., Zhou, C., Ma, P., Li, C., Lu, Q., ... & Wang, H. (2022). Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken. Journal of nanobiotechnology, 20(1), 167.
• Güner, A., Özhatay, N., Ekim, T., Başer, K. H. C., & Hedge, I. C. (Eds.). (2000). Flora of Turkey and the east Aegean Islands. Edinburgh University Press.
• Güner, A., Aslan, S., Ekim, T., Vural, M., & Babaç, M. T. (2012). Türkiye bitkileri listesi. Damarlı Bitkiler, Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını, 262.
• Jiang, W. Z., Henry, I. M., Lynagh, P. G., Comai, L., Cahoon, E. B., & Weeks, D. P. (2017). Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa, using CRISPR/Cas9 gene editing. Plant biotechnology journal, 15(5), 648-657.
• Jones, F. A. (1996). Herbs–useful plants. Their role in history and today. European journal of gastroenterology & hepatology, 8(12), 1227-1231.
• Keles, O., AK, S., Bakırel, T., & Alpınar, K. (2001). Türkiye'de yetişen bazı bitkilerin antibakteriyel etkisinin incelenmesi. Turkish Journal of Veterinary and Animal Sciences, 25(4), 559-565.
• Li, Z. H., Cai, M., Liu, Y. S., Sun, P. L., & Luo, S. L. (2019). Antibacterial activity and mechanisms of essential oil from Citrus medica L. var. sarcodactylis. Molecules, 24(8), 1577.
• Mahboubi, M., & Bidgoli, F. G. (2010). In vitro synergistic efficacy of combination of amphotericin B with Myrtus communis essential oil against clinical isolates of Candida albicans. Phytomedicine, 17(10), 771-774.
• Nogueira, T., Marcelo-Curto, M. J., Figueiredo, A. C., Barroso, J. G., Pedro, L. G., Rubiolo, P., & Bicchi, C. (2008). Chemotaxonomy of Hypericum genus from Portugal: Geographical distribution and essential oils composition of Hypericum perfoliatum, Hypericum humifusum, Hypericum linarifolium and Hypericum pulchrum. Biochemical Systematics and Ecology, 36(1), 40-50.
• Palombo, E. A. (2011). Traditional medicinal plant extracts and natural products with activity against oral bacteria: potential application in the prevention and treatment of oral diseases. Evidence-based complementary and Alternative Medicine, 2011.
• Perez, C. (1990). Antibiotic assay by agar-well diffusion method. Acta Biol Med Exp, 15, 113-115.
• Rančić, A., Soković, M., Vukojević, J., Simić, A., Marin, P., Duletić-Laušević, S., & Djoković, D. (2005). Chemical composition and antimicrobial activities of essential oils of Myrrhis odorata (L.) Scop, Hypericum perforatum L and Helichrysum arenarium (L.) Moench. Journal of Essential Oil Research, 17(3), 341-345.
• Robson, N. K. B. (2001). Studies in the genus Hypericum L.(Guttiferae) 4 (1). Sections 7. Roscyna to 9. Hypericum sensu lato (part 1). Bulletin of the Natural History Museum Botany Series, 31, 37-88.
• Russo, A., Formisano, C., Rigano, D., Senatore, F., Delfine, S., Cardile, V., ... & Bruno, M. (2013). Chemical composition and anticancer activity of essential oils of Mediterranean sage (Salvia officinalis L.) grown in different environmental conditions. Food and Chemical Toxicology, 55, 42-47.
• Sezik, E., Yesilada, E., Shadidoyatov, H., Kulivey, Z., Nigmatullaev, A. M., Aripov, H. N., ... & Honda, G. (2004). Folk medicine in Uzbekistan: I. Toshkent, Djizzax, and Samarqand provinces. Journal of Ethnopharmacology, 92(2-3), 197-207.
• Sharma, A., Bajpai, V. K., & Baek, K. H. (2013). Determination of antibacterial mode of action of a llium sativum essential oil against foodborne pathogens using membrane permeability and surface characteristic parameters. Journal of Food Safety, 33(2), 197-208.
• Steel, R. G., & Torrie, J. H. (1980). Principles and procedures of statistics mcgraw-hill book co. Inc., New York, 481.
• Torğut, G., Yazdıç, F. C., & Gürler, N. (2022). Synthesis, characterization, pH‐sensitive swelling and antimicrobial activities of chitosan–graft‐poly (hydroxyethyl methacrylate) hydrogel composites for biomedical applications. Polymer Engineering & Science, 62(8), 2552-2559.
• Valgas, C., Souza, S. M. D., Smânia, E. F., & Smânia Jr, A. (2007). Screening methods to determine antibacterial activity of natural products. Brazilian journal of microbiology, 38, 369-380.
• Xiang, F., Bai, J., Tan, X., Chen, T., Yang, W., & He, F. (2018). Antimicrobial activities and mechanism of the essential oil from Artemisia argyi Levl. et Van. var. argyi cv. Qiai. Industrial crops and products, 125, 582-587.
• Zinoviadou, K. G., Koutsoumanis, K. P., & Biliaderis, C. G. (2009). Physico-chemical properties of whey protein isolate films containing oregano oil and their antimicrobial action against spoilage flora of fresh beef. Meat Science, 82(3), 338-345.