Genome-wide Development and Physical Mapping of SSR Markers in Sugar Beet (Beta vulgaris L.)

Year 2023, Volume: 13 Issue: 1, 112 - 119, 01.03.2023

İbrahim Çelik

https://doi.org/10.21597/jist.1187003

Cited By: 1

Abstract

Sugar beet (Beta vulgaris L.) is one of the important sugar crops. Thus, development of
molecular tools to understand molecular mechanism of agronomic traits such as root yield and
sucrose content by using comprehensive genome analysis is important for sugar beet molecular
breeding. A prime to such a comprehensive genome analysis is high throughout marker
development. In the present study, mining of sugar beet genome for simple sequence repeats
(SSRs) revealed 37704 motifs. Dinucleotide repeats were the most abundant accounting for
69.3 %. A total of 22500 SSR primers were developed and these markers were physically
mapped in sugar beet genome. The markers distributed over nine chromosomes and the map
had a higher resolution (16.73 kb SSR-1). Also 102 SSR markers were found to be putatively
associated with carbohydrate synthesis. The present study is the first report of genome-wide
development of SSR markers and construction of SSR based physical map in sugar beet. SSR
markers increased the number of available sugar beet specific SSR markers by 55-fold. SSR
markers developed in the present study are valuable molecular genetic tools for comprehensive
genome analysis to facilitate sugar beet molecular breeding

Keywords

Genomics, Beta vulgris L., genomic SSR, functional annotation

Supporting Institution

Çalışmayı destekleyen kurum yoktur.

Project Number

-

Thanks

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