Uçucu Yağlar İçeren Kitosan Bazlı Filmlerin Antimikrobiyal Aktivitelerinin İncelenmesi

Year 2023, Volume: 13 Issue: 1, 143 - 151, 01.03.2023

Sevim Feyza Erdoğmuş , Özlem Erdal Altıntaş

https://doi.org/10.21597/jist.1200424

Abstract

Bu çalışmada, kitosan bazlı film üretilerek bu filmlerin yüzey özellikleri incelenmiş ve en uygun
formülasyon belirlenmiştir. Film yapısının karakterizasyonu, taramalı elektron mikroskobu,
fourier dönüşümlü kızılötesi spektroskopisi, x-ışınları difraksiyonu analizleri yapılarak
değerlendirilmiştir. Bu formülasyon farklı konsantrasyonlarda çay ağacı ve\veya melisa uçucu
yağı ilavesi yapılarak antimikrobiyal etkinlikleri karşılaştırılmıştır. Filmler mikroyapı açısından
değerlendirildiğinde homojen ve esnek bir yüzey yapısı oluşturmuştur. Film yapılarının
antimikrobiyal aktivitesi Listeria monocytogenes ATCC 19115, Staphylococcus aureus ATCC
25923, Klebsiella pneumoniae NRRLB 4420, Enterococcus faecalis ATCC 51289, Escherichia
coli ATCC 35218, Bacillus subtilis NRS 744, Staphylococcus aureus ATCC 6538, Escherichia
coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC
12600, Pseudomonas aeruginosa ATCC 11778 ve Candida albicans ATCC 10231 üzerinde test
edilmiştir. Test edilen film yapıları patojen mikroorganizmalar üzerinde antimikrobiyal etki
göstermiştir. Uçucu yağların antimikrobiyal aktiviteyi arttırdığı belirlenmiştir.

Keywords

Antimikrobiyal aktivite, Kitosan film, Uçucu yağ

Investigation of Antimicrobial Activities of Chitosan Based Films That Contain Esential Oils

Abstract

In this study, chitosan based films were produced and the most suitable formula in terms of
surface properties was determined. The characterization of the film layer was evaluated by
scanning electron microscopy, Fourier transform infrared spectroscopy, x-ray diffraction
analyses. Different concentrations of tea tree and/or lemon balm essential oil were added to this
formula and their antimicrobial activities were compared. When the films were evaluated in
terms of microstructure, they formed a homogeneous and flexible surface structure.
Antimicrobial activities of films were tested on Listeria monocytogenes ATCC 19115,
Staphylococcus aureus ATCC 25923, Klebsiella pneumoniae NRRLB 4420, Enterococcus
faecalis ATCC 51289, Escherichia coli ATCC 35218, Bacillus subtilis NRS 744,
Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Pseudomonas aeruginosa
ATCC 27853, Staphylococcus aureus ATCC 12600, Pseudomonas aeruginosa ATCC 11778 and
Candida albicans ATCC 10231. The tested film structures showed an antimicrobial effect on the
pathogenic microorganisms. It has been determined that essential oils increase the antimicrobial
activity.

Keywords

Antimicrobial activity, Chitosan film, Esential oil

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