Bazı Azo Boyalarının QSAR Yöntemi ve Daphnia Magna ile Akut Toksisite Testi ile İncelenmesi

Year 2023, Volume: 13 Issue: 2, 1110 - 1119, 01.06.2023

Yasemin Keşkek Karabulut , Yelda Yalçın Gürkan

https://doi.org/10.21597/jist.1214772

Abstract

Azo boyalar, tekstil boyalarının en önemli sınıfını temsil eder. Azo boyaların biyotransformasyonu aromatik aminleri serbest bırakabilir. Bazı aromatik aminlerin genotoksik ve/veya kanserojen özelliklere sahip olduğu iyi bilinmektedir. Bu çalışmada, tekstil sektöründe yaygın olarak kullanılan aromatik aminlerden oluşan azo boyalarının çevre üzerindeki etkilerinin akut su toksisitesi testi ile araştırılması amaçlanmıştır. Daphnia Magna kullanılarak yapılan akut su toksisite testi deneysel ve teorik olarak incelenmiştir. Teorik çalışmalarda son zamanlarda ilgi gören ve ülkeler arası yönetmeliklerde de kullanılan OECD QSAR Toolbox programı tercih edilmiştir. Yapılan çalışmalar sonucunda deneysel ve teorik sonuçların paralel sonuçlar verdiği görülmüştür. Bu çalışma, Daphnia Magna üzerinde yapılan akut toksisite testlerinde maliyeti, süreyi ve hayvan ölümlerini azaltmak amacıyla teorik çalışmaların deneysel çalışmalara ikame olabileceğini göstermiştir. Ayrıca, bu çalışma sonuçlarına göre Basic Yellow 28, Dispers Blue 291 ve Dispers Brown 27-1 azo boyalarının ekotoksikolojik olarak toksik olduğu deneysel ve teorik yöntemler ile bulunmuştur

Keywords

Aromatik aminler, Azo boyaları, QSAR, Teorik kimya, Daphnia Magna, Ekotoksikoloji

Supporting Institution

Setaş Kimya San. A.Ş.

Project Number

BY18-0005

Thanks

Bu çalışma, SETAŞ Kimya San. A.Ş. tarafından Arge Merkezi Bakanlık Projeleri kapsamında BY18-0005 proje numarası ile desteklenmiştir.

Investigation of Some Azo Dyes by QSAR Method and Acute Toxicity Test with Daphnia Magna

Abstract

Azo dyes represent the most important class of textile dyes. Biotransformation of azo dyes can release aromatic amines. It is well known that some aromatic amines have genotoxic and/or carcinogenic properties. In this study, it was aimed to investigate the effects of azo dyes, which are composed of aromatic amines, and are widely used in the textile industry, on the environment using acute water toxicity test. The acute water toxicity test using Daphnia Magna was investigated experimentally and theoretically. The OECD QSAR Toolbox program, which has recently attracted attention in theoretical studies and is also used in international regulations, has been preferred. As a result of the studies, it was seen that the experimental and theoretical results gave parallel results. This study showed that theoretical studies can be substituted for experimental studies in order to reduce cost, time, and animal mortality in acute toxicity tests on Daphnia Magna. In addition, according to the results of this study, it was found by experimental and theoretical methods that Basic Yellow 28, Disperse Blue 291, and Disperse Brown 27-1 azo dye are ecotoxicologically toxic.

Keywords

Aromatic amines, Azo Dyes, QSAR, Theoretical chemistry, Daphnia Amgna, Ecotoxicology

Project Number

BY18-0005

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