Onobrychis argyrea subsp. argyrea Ekstrelerinin Antioksidan, Antimikrobiyal ve Antiproliferatif Aktivitelerinin Belirlenmesi

Year 2023, Volume: 13 Issue: 2, 1134 - 1141, 01.06.2023

Sevgi Altın , Cemalettin Alp , Ekrem Köksal , Sümeyye Akyüz

https://doi.org/10.21597/jist.1240818

Cited By: 4

Abstract

Fabaceae familyasına ait bitkiler, hayvan beslenmesinde ve insan sağlığının korunmasında (antidiyabetik,
antikanserojen, antioksidan, antienflamatuar ve kardiyovasküler) büyük öneme sahiptir. Bu familyanın
önemli cinslerinden biri olan Onobrychis yüksek fenolik içeriğe sahiptir ve güçlü bir antioksidan,
antikanserojen ve antimikrobiyal ajan olarak bilinir. Bu çalışmada, Onobrychis argyrea subsp. argyrea
(O. argyrea) türünün kloroform (OAC), etanol (OAE) ve su (OAW) ekstreleri hazırlanmış ve bu
ekstrelerin antiproliferatif, antimikrobiyal ve antioksidan aktiviteleri incelenmiştir. Ekstraktların
antiproliferatif etkileri farklı kanser hücre dizileri üzerinde XTT kolorimetrik yöntemi ile
değerlendirilmiştir. Bulgulara göre, OAE ekstresi tüm hücre hatlarına karşı en yüksek etkinliği
göstermiştir. MCF-7 %83.71, A549 %92.14 ve HT-29 %72.24 inhibe edildi. Tüm ekstrelerin
Staphylococcus aureus, Escherichia coli ve Streptococcus pneumoniae'ye karşı antimikrobiyal aktivitesi,
disk difüzyon tekniği kullanılarak belirlendi. Son olarak antioksidan gücü DPPH ve FRAP yöntemleri
kullanılarak belirlendi. DPPH yönteminde, OAE eksrtesi en yüksek radikal süpürme aktivitesi gösterdi
(IC50: 34.12±0.2). OAE (257.95±1.40) ve OAW (282.14±0.83) ekstreleri, FRAP yönteminde benzer
sonuçlarda yüksek aktivite göstermiştir. Toplam fenolik madde içeriği OAE için 226.15±1.89 mg GAE/g
ve OAW için 146.52±0.71 mg GAE/g olarak belirlendi. Bu sonuçlar, çalışılan bitkinin önemli bir
antioksidan kaynağı olduğunu göstermektedir. Bütün bu sonuçlardan bitkinin çeşitli biyolojik aktivitelere
ve zengin ikincil metabolitlere sahip olduğu anlaşılmaktadır.

Keywords

Onobrychis argyrea subsp. argyrea, antioksidan, antiproliferatif, antimikrobial

Determination of Antioxidant, Antimicrobial, and Antiproliferative Activities of Onobrychis argyrea subsp. argyrea Extracts

Abstract

Plants belonging to the Fabaceae family have great importance in animal nutrition and the protection of human health (antidiabetic, anticarcinogenic, antioxidant, anti-inflammatory, and cardiovascular). Onobrychis, known as a potent antioxidant, anticarcinogenic and antimicrobial agent, its high phenolic substance is one of the important genera of this family. In this study, chloroform (OAC), ethanol (OAE), and water (OAW) extract of Onobrychis argyrea subsp. argyrea (O. argyrea) specie was prepared; and the antiproliferative, antimicrobial, and antioxidant activities were investigated. The antiproliferative effects of the extracts were evaluated on different cancer cell lines by XTT colourimetric method. OAE demonstrated the most excellent efficacy against all cell lines, according to the findings. MCF-7 83.71%, A549 92.14%, and HT-29 72.24% were all inhibited. The antimicrobial activity of OAE and OAW extracts against Staphylococcus aureus, Escherichia coli, and Streptococcus pneumoniae was determined using the disc-diffusion technique. Finally, antioxidant power was determined using DPPH and FRAP methods. In the DPPH research, OAE and OAW extracts showed the highest levels of inhibition (IC50: 34.12±0.2 and 21.58±0.12, respectively). OAE (257.95±1.40) and OAW (282.14±0.83) extracts showed
high activity with similar results in the FRAP method. Total phenolic content was determined as 226.15±1.89 mg GAE/g for OAE and 146.52±0.71 mg GAE/g for OAW, respectively. These results show that the species is an essential source of antioxidants. These studies are the first data for Onobrychis argyrea subsp. argyrea. All these results show that the plant has various biological activities and rich
secondary metabolites.

Keywords

Onobrychis argyrea subsp. argyrea, antioxidant, antiproliferative, antimicrobial

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