Farklı Sıcaklık ve Sürelerde Bekletilen Kasımpatı Polenlerinde Polen Kalitesinin Belirlenmesi

Year 2023, Volume: 13 Issue: 4, 2303 - 2314, 01.12.2023

Hilal Dursun , Soner Kazaz , Tuğba Kılıç

https://doi.org/10.21597/jist.1290665

Abstract

Polen kalitesi, tohum oluşumu üzerindeki etkilerinden dolayı ıslah programlarında en önemli faktörlerden biridir. Etkili bir ıslah programı için ıslahçılar, polenin canlılığını, çimlenme oranını ve polenlerin canlılığını koruma süresini mutlaka bilmelidir. Bu çalışma, farklı sıcaklık ve sürelerde bekletilen 'Chic' ve 'Barolo' sprey kasımpatı çeşitlerinin polen canlılığı ve çimlenme oranlarını belirlemek amacıyla yapılmıştır. Her iki çeşide ait polenler, tam otomasyonlu bir ıslah serasında yetiştirilen bitkilerden elde edilmiştir. Polenler, +24°C’de ve +4°C’de 7 gün boyunca bekletilmiştir. 0. gün dahil olmak üzere 8 gün boyunca günlük olarak TTC yöntemi ile polen canlılıkları, modifiye ME3-m ortamı ile asılı damla yönteminde ise çimlenme oranları belirlenmiştir. Elde edilen bulgulara göre, 'Chic' çeşidinin 'Barolo' çeşidinden daha fazla canlı polene sahip olduğu saptanmıştır. Canlı polen ve çimlenme oranları, bekletme sürelerinin artmasıyla sürekli olarak azalma eğilimi göstermiştir. 7. günde çimlenme oranı 'Barolo' ve 'Chic' çeşitlerinde sırasıyla %93.44 ve %71.64 oranında azalmıştır. Ancak +4°C’de tutulan polenin canlılığını koruma kapasitesi her iki çeşitte de daha iyi bulunmuştur. Çalışmada, polenlerin öncelikle taze olarak uygulanması, gerektiğinde +4°C’de saklanarak kullanılması, +24°C'de bekletilen polenlerin ise 2 güne kadar kullanılması gerektiği sonucuna varılmıştır. +4°C’de bu süre çeşide göre 4 güne kadar uzayabilir.

Keywords

Krizantem, Canlı polen oranı, TTC yöntemi, Monnier, Asılı damla

Pollen Quality of Some Spray Chrysanthemum Varieties at Different Holding Time and Temperature

Abstract

Pollen quality is one of the most important factors in breeding programs because of its effects on seed formation. For an effective breeding program, breeders must know pollen viability, germination rate, and the duration of maintaining pollen viability. This study was carried out to determine pollen viability and germination rates of 'Chic' and 'Barolo' spray chrysanthemum varieties kept at different temperatures and times. Pollen from both varieties was obtained from plants grown in a fully automated greenhouse. Pollens were stored at +24°C and +4°C for 7 days. Pollen viability was determined by the TTC method and germination rates were determined by the hanging drop method with modified ME3-m medium, daily for 8 days, including day 0. The 'Chic' varieties had more viable pollen than the 'Barolo' variety. Viable pollen and germination rates tended to decrease continuously with time. On the 7th day, the germination rate decreased by 93.44% and 71.64% in the 'Barolo' and 'Chic' varieties, respectively. However, the capacity to maintain the viability of pollen kept at +4°C was found to be better in both varieties. In the study, it was concluded that the pollen should be applied freshly but stored at +4°C when necessary. The pollen kept at +24°C can be used for up to 2 days. This period can be extended up to 4 days at +4°C, depending on the variety.

Keywords

Mums, Viable pollen rate, TTC methods, Monnier, Hanging drop

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