Duman ve Duman Kökenli Bileşiklerin Liquidambar orientalis Mill. (Anadolu Sığla Ağacı)'in Tohum Çimlenmesi Üzerindeki Etkileri

Year 2023, Volume: 13 Issue: 4, 2393 - 2402, 01.12.2023

Şükrü Serter Çatav , Kenan Akbaş

https://doi.org/10.21597/jist.1309431

Abstract

Duman ve duman kökenli bileşikler, bitkilerde tohum çimlenmesi, somatik embriyogenez ve çiçeklenme gibi fizyolojik ve gelişimsel süreçleri düzenleyebilmektedir. Dumanla ilişkili çimlenme yanıtları, karrikinler, gliseronitril, siringaldehit, katekol ve 3,4,5-trimetilfuran-2(5H)-on gibi uyarıcı ve inhibe edici bileşiklerin mevcudiyeti nedeniyle oldukça karmaşık bir yapıya sahiptir. Bu çalışmada, duman suyu, karrikinolid (KAR1), mandelonitril (MAN) ve katekol (KAT) uygulamalarının Liquidambar orientalis Mill.'in tohum çimlenmesi üzerindeki etkileri incelenmiştir. Ayrıca KAR1 ve siyanohidrin ile indüklenen tohum çimlenmesinde rol aldıkları düşünülen giberellik asit (GA3) ve reaktif oksijen türleri de (ROT) deneylere dâhil edilmiştir. ROT uygulamaları için hidrojen peroksit ve metil violojen çözeltileri kullanılmıştır. Çimlenme deneyleri, 20 °C'ye ayarlanmış inkübatörlerde farklı ışık koşulları altında gerçekleştirilmiştir. Çalışmanın sonuçları, L. orientalis tohumlarının dumana karşı duyarlı olduğunu göstermiştir. Buna ek olarak, 0.01 ve 0.1 µM KAR1 ve 50 µM MAN solüsyonları hem karanlık hem de aydınlık koşullar altında çalışma türünün çimlenmesini teşvik etmiştir (p < 0.05). Öte yandan, KAT uygulamaları çimlenme üzerinde olumlu veya olumsuz bir etki yaratmamıştır. KAR1 ve siyanohidrine hassas olan L. orientalis tohumları 10-5 ila 10-3 M konsantrasyon aralığında GA3'e karşı pozitif bir çimlenme cevabı vermiştir. GA3 uygulamaları yüksek çimlenme başarısı için ışığa olan gereksinimi de ortadan kaldırmıştır. ROT ile uyarılan çimlenme ise sadece ışık varlığında gözlemlenmiştir. Bu çalışmadan elde edilen bulgular, dar yayılışlı bir tür olan L. orientalis'in çimlenme başarısını arttırmak için kullanılabilir.

Keywords

Duman suyu, Karrikinolid, Giberellik asit, Reaktif oksijen türleri, Siyanohidrin

Supporting Institution

Muğla Sıtkı Koçman Üniversitesi Öğretim Üyesi Yetiştirme Programı (ÖYP) Koordinatörlüğü

The Effects of Smoke and Smoke-Derived Compounds on Seed Germination of Liquidambar orientalis Mill. (Anatolian Sweetgum Tree)

Abstract

Smoke and smoke-derived compounds can regulate physiological and developmental processes, such as seed germination, somatic embryogenesis, and flowering in plants. Smoke-related germination responses are highly complex due to the presence of stimulatory and inhibitory compounds, such as karrikins, glyceronitrile, syringaldehyde, catechol, and 3,4,5-trimethylfuran-2(5H)-one. In this study, the effects of smoke water, karrikinolide (KAR1), mandelonitrile (MAN), and catechol (CAT) treatments on seed germination of Liquidambar orientalis Mill. were investigated. Moreover, gibberellic acid (GA3) and reactive oxygen species (ROS), which are thought to play a role in KAR1- and cyanohydrin-induced seed germination, were also included in the experiments. Hydrogen peroxide and methyl viologen solutions were used for ROS treatments. Germination experiments were carried out under different light conditions in incubators set at 20 °C. The results of the study showed that L. orientalis seeds were sensitive to smoke. In addition, 0.01 and 0.1 µM KAR1 and 50 µM MAN solutions promoted the germination of the study species under both dark and light conditions (p < 0.05). On the other hand, CAT treatments did not have a positive or negative effect on germination. L. orientalis seeds sensitive to KAR1 and cyanohydrin gave a positive germination response to GA3 in the concentration range of 10-5 to 10-3 M. GA3 treatments also eliminated the requirement of light for high germination success. ROS-induced germination was only observed in the presence of light. The findings of this study can be used to increase the germination success of L. orientalis, a narrowly distributed species.

Keywords

Smoke-water, Karrikinolide, Gibberellic acid, Reactive oxygen species, Cyanohydrin

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