Antibiotic resistance patterns of STEC and ETEC strains: A study on frozen foods of animal origin and children with acute diarrhea

Abstract

Objective: Shigatoxin producing E. coli (STEC) and Enterotoxigenic E. coli (ETEC) are infectious pathogens that have been implicated in food and waterborne diseases in human all the world. The aim of this study was to determine the incidence and evaluate antibiotic resistance patterns of STEC and ETEC strains. Methods: In total 125 frozen foods of animal origin and 466 rectal swabs from children with acute diarrhea were taken to isolate and identify E. coli strains based on standard procedures. Antimicrobial susceptibility tests for E. coli strains were performed according to the Clinical Laboratory Standards Institute. Resistance to two or more classes of antimicrobials in STEC and ETEC strains was recorded. Results: A total of 87 strains of E. coli strains were detected from 466 rectal swabs from children with acute diarrhea and 40 strains of E. coli strains were detected from the 125 frozen food samples of animal origin. Test results indicated a 5.0% and 2.5% prevalence Shiga toxin (stx) and enterotoxin (estA) genes among E. coli strains isolated from frozen foods of animal origin. Similarly, 5.7% and 4.5% prevalence of Shiga toxin (stx) and enterotoxin (estA) genes among E. coli strains isolated from children. Conclusion: We conclude that E. coli stains having Shiga toxin (stx), and enterotoxin (estA) genes considered not to be a potential source of infections for in Sanandaj; perhaps other enteric pathogens are the major cause of food-borne diseases.

Keywords

• E. coli, acute diarrhea, children, frozen food sample, stx and estA gene

Antibiotic resistance patterns of STEC and ETEC strains: A study on frozen foods of animal origin and children with acute diarrhea

Öz

Amaç: Shigatoxin üreten E. coli (STEC) ve Enterotoksijenik E. coli (ETEC) tüm dünyada gıda ve su kaynaklı insan hastalıklarından sorumlu tutulan enfeksiyöz patojenlerdir. Bu çalışmanın amacı, STEC ve ETEC suşlarının insidansını belirlemek ve antibiyotik direnç paternlerini değerlendirmektir.Yöntemler: E. coli suşlarını izole edilmesi ve standart prosedürler esas alınarak tanımlanması için hayvansal kökenli toplam 125 dondurulmuş gıda ve akut ishali olan çocuklardan 466 rektal sürüntü alındı. E. coli suşlarının antimikrobiyal duyarlılık testleri Klinik Laboratuvar Standartları Enstitüsü’ne göre yapıldı. STEC ve ETEC suşlarında iki ya da daha fazla antimikrobiyal gruba karşı direnç varlığı kaydedildi.Bulgular: Akut ishali olan çocuklardan alınan 466 rektal sürüntü örneğinden toplam 87 E. coli suşu ve 125 dondurulmuş hayvansal kökenli gıda örneğinden 40 E. coli suşu tespit edildi. Test sonuçları hayvansal kökenli dondurulmuş gıdalardan izole edilen E. coli suşlarındaki Shiga toksin (stx) ve enterotoksin (estA) genlerinin prevalansını %5,0 ve %2,5 olarak gösterdi. Benzer şekilde, çocuklardan izole edilen E. coli suşlarında Shiga toksin (stx) ve enterotoksin (estA) genlerinin prevalansı ise %5,7 ve %4,5 idi.Sonuç: Shiga toksin (stx), ve enterotoksin (estA) genleri bulunduran E. coli suşlarının, Sanandaj için potansiyel bir enfeksiyon kaynağı olmadığı sonucuna vardık; belki de gıda kaynaklı hastalıkların en önemli nedeni diğer enterik patojenlerdir

Anahtar Kelimeler

• E. coli, akut ishal, çocuklar, dondurulmuş gıda örneği, stx ve estA geni

References

1. Beutin L, Krause G, Zimmermann S, Kaulfuss S, Gleier K. Characterization of Shiga Toxin-Producing Escherichia coli Strains Isolated from Human Patients in Germany over a 3-Year Period. J Clin Microbiol 2004;42:1099-1108.
2. World Health Organization Scientific Working Group: Zoonotic non-O157 Shiga toxin-producing Escherichia coli (STEC), 23-26 June 1998, Berlin, Germany, 1-30. W.H.O./CSR/ APH/98.8. World Health Organization, Geneva, Switzerland.
3. Rani U, Ayesha M F, Viswanath P, Sandhu JS. Studies on detection, enumeration and survival of E. coli ‘‘ready to eat” processed cereal food product. Asian J Microbiol Biotechnol Environ Sci 2005;7:395-400.
4. Kaper JB, Nataro JP, Mobley HL. Pathogenic Escherichia coli. Nat Rev Microbiol 2004;2:123-140.
5. Nguyen TV, Le Van P, Le Huy C, Gia KN, Weintraub A. De- tection and Characterization of Diarrheagenic Escherichia coli from Young Children in Hanoi, Vietnam. J Clin Microb 2005;3:755-760.
6. World Health Organization (WHO). Food safety and food- borne illness. WHO fact sheet No. 237. 2002; http://www. who.int/mediacentre/factsheets/fs237/en/.
7. FAO/WHO: Food borne disease surveillance in Japan. 2004. http://www.fao.org/docrep/meeting/008/ae340e/ae340e00. htm.
8. Khorramizadeh MR, Soltan-Dallal MM, Safavifar F, et al. As- sessment of Ail Gene Marker Amplicon for Molecular Char- acterization of Pathogenic Yersinia enterocolitica in Food Samples Collected in Iran. Iranian J Publ Health 2007;36;8- 15.

9. Boerlin P, Travis R, Gyles C, et al. Antimicrobial resistance and virulence genes of Escherichia coliisolates from swine in Ontario. Appl. Environ. Microbiol 2005;71:6753-6761.
10. Choi C, Kwon D, Chae C. Prevalence of the enteroaggrega- tive Escherichia coli heat-stable enterotoxin gene and its re- lationship with fimbrial and enterotoxin genes in E. coli isolat- ed from diarrheic piglets. J Vet Diagn Invest 2001;13:26-29.
11. Aslani MM, Alikhani MY. Molecular and phenotypic charac- terization of atypical enteropathogenic Escherichia coli se- rotypes isolated from children with and without diarrhea. J Microbiol Immunol Infect 2011;44:27-32.
12. Institute of Standards and Industrial Research of Iran. Meth- od for isolation and identification of E. coli. 2946, 1995.
13. Baron J, Fingold S. Methods for identification of etiologic agents of infectious diseases. In: Bailly’s and Scotts Diag- nostic Microbiology. 10th Ed. Mosby Inc: New York; 1996.
14. Kalantar E, Soheili F, Salimi H, Soltan-Dallal MM. Frequency, antimicrobial susceptibility and plasmid profiles of Escherich- ia coli pathotypes obtained from children with acute diarrhea. Jundishapur J Microbiol 2011;4:23-28.
15. Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing; 20th infor- mational supplement. CLSI/NCCLS M100-S20. 2010; 3 (1): Clinical and Laboratory Standards Institute, Wayne, Pa.
16. Lin Z, Kurazono H, Yamasaki S, Takeda Y. Detection of various variant verotoxin genes in Escherichia coli by poly- merase chain reaction. Microbiol Immunol 1993;37:543-548.
17. Rappelli P, Maddau G, Mannu F, Colombo MM, Fiori PL, Cappuccinelli P. Development of a set of multiplex PCR as- says for the simultaneous identification of enterotoxigenic, enteropathogenic, enterohemorrhagic and enteroinvasive Escherichia coli. New Microbiol 2001;24:77-83.
18. Centers for Disease Control and Prevention. Preliminary foodnet data on the incidence of foodborne illnesses- selected sites, United States, 2001. Morb Mortal Wkly 2002;51:325-329.
19. Aslani MM, Bouzari S. An epidemiological study on verotoxin- producing Escherichia coli (VTEC) infection among popula- tion of northern region of Iran (Mazandaran and Golestan provinces). Eur J Epidemiol 2003;18:345-349.
20. Alikhani MY, Mirsalehian A, Fatollahzadeh B, Pourshafie MR, Aslani MM. Prevalence of Enteropathogenic and Shiga Toxin-producing Escherichia coli among Children with and without diarrhoea in Iran. J Health Popul Nutr 2007;25:88-93.
21. WHO - World Health Organization. Reducing Risks, Pro- moting Healthy Life. The World Health Report. 2002; (http:// www.who.int/whr/en/).
22. Zamboni A, Fabbricotti SH, Fagundes-Neto U, Scaletsky IC. Enteroaggregative Escherichia coli virulence factors are found to be associated with infantile diarrhea in Brazil. J Clin Microbiol 2004;42:1058-1063.
23. Aslani MM, Ahrabi SS, Alikhani YM, Jafari F, Zali RM, Mani M. Molecular detection and antimicrobial resistance of diarrhea- genic Escherichia coli strains isolated from diarrheal cases. Saudi Med J 2008;29:388-92.
24. Mainil J. Shiga/verocytotoxin and Shiga/verotoxigenic Esch- erichia coli in animals. Vet Res 1999;30:235-257.