Antibiotic resistance patterns of STEC and ETEC strains: A study on frozen foods of animal origin and children with acute diarrhea

Abstract

Objective: Shigatoxin producing E. coli (STEC) and Enterotoxigenic E. coli (ETEC) are infectious pathogens that have been implicated in food and waterborne diseases in human all the world. The aim of this study was to determine the incidence and evaluate antibiotic resistance patterns of STEC and ETEC strains. Methods: In total 125 frozen foods of animal origin and 466 rectal swabs from children with acute diarrhea were taken to isolate and identify E. coli strains based on standard procedures. Antimicrobial susceptibility tests for E. coli strains were performed according to the Clinical Laboratory Standards Institute. Resistance to two or more classes of antimicrobials in STEC and ETEC strains was recorded. Results: A total of 87 strains of E. coli strains were detected from 466 rectal swabs from children with acute diarrhea and 40 strains of E. coli strains were detected from the 125 frozen food samples of animal origin. Test results indicated a 5.0% and 2.5% prevalence Shiga toxin (stx) and enterotoxin (estA) genes among E. coli strains isolated from frozen foods of animal origin. Similarly, 5.7% and 4.5% prevalence of Shiga toxin (stx) and enterotoxin (estA) genes among E. coli strains isolated from children. Conclusion: We conclude that E. coli stains having Shiga toxin (stx), and enterotoxin (estA) genes considered not to be a potential source of infections for in Sanandaj; perhaps other enteric pathogens are the major cause of food-borne diseases.

Keywords

• E. coli, acute diarrhea, children, frozen food sample, stx and estA gene

Antibiotic resistance patterns of STEC and ETEC strains: A study on frozen foods of animal origin and children with acute diarrhea

Öz

Amaç: Shigatoxin üreten E. coli (STEC) ve Enterotoksijenik E. coli (ETEC) tüm dünyada gıda ve su kaynaklı insan hastalıklarından sorumlu tutulan enfeksiyöz patojenlerdir. Bu çalışmanın amacı, STEC ve ETEC suşlarının insidansını belirlemek ve antibiyotik direnç paternlerini değerlendirmektir.Yöntemler: E. coli suşlarını izole edilmesi ve standart prosedürler esas alınarak tanımlanması için hayvansal kökenli toplam 125 dondurulmuş gıda ve akut ishali olan çocuklardan 466 rektal sürüntü alındı. E. coli suşlarının antimikrobiyal duyarlılık testleri Klinik Laboratuvar Standartları Enstitüsü’ne göre yapıldı. STEC ve ETEC suşlarında iki ya da daha fazla antimikrobiyal gruba karşı direnç varlığı kaydedildi.Bulgular: Akut ishali olan çocuklardan alınan 466 rektal sürüntü örneğinden toplam 87 E. coli suşu ve 125 dondurulmuş hayvansal kökenli gıda örneğinden 40 E. coli suşu tespit edildi. Test sonuçları hayvansal kökenli dondurulmuş gıdalardan izole edilen E. coli suşlarındaki Shiga toksin (stx) ve enterotoksin (estA) genlerinin prevalansını %5,0 ve %2,5 olarak gösterdi. Benzer şekilde, çocuklardan izole edilen E. coli suşlarında Shiga toksin (stx) ve enterotoksin (estA) genlerinin prevalansı ise %5,7 ve %4,5 idi.Sonuç: Shiga toksin (stx), ve enterotoksin (estA) genleri bulunduran E. coli suşlarının, Sanandaj için potansiyel bir enfeksiyon kaynağı olmadığı sonucuna vardık; belki de gıda kaynaklı hastalıkların en önemli nedeni diğer enterik patojenlerdir

Anahtar Kelimeler

• E. coli, akut ishal, çocuklar, dondurulmuş gıda örneği, stx ve estA geni

Kaynakça

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