Non-typhoidal Salmonella isolates from livestock and food samples, Kolkata, India

Year 2016, Volume: 6 Issue: 3, 113 - 120, 01.09.2016

Sudhanthiramani Sudhanthirakodi

https://doi.org/10.5799/jmid.vi.328974

Cited By: 6

Abstract

Objective: Non-typhoidal Salmonella (NTS) is a major foodborne pathogen in both developed and developing countries.
The study was undertaken to determine the prevalence, serotype distribution and antimicrobial resistance profiles
of NTS isolates from livestock and food samples collected in and around Kolkata during 2012-2013.
Materials and methods: A total of 500 samples (animal, poultry and raw food) from 12 different places randomly collected
in and around Kolkata were processed as per standard procedures and analyzed for NTS. Serotyping of the isolates
was done followed by determination of antimicrobial resistance profiles and Minimum inhibitory concentrations
following disc diffusion and E-test methods.
Results: A total of 53 (10.6%) NTS isolates were recovered, with highest isolation from chicken (40%), followed by beef
and poultry cloacal samples (22% each). Total 11 Salmonella serovars were identified with predominance of Salmonella
enterica serovar Typhimurium (S. Typhimurium) (30.2%) followed by S. Kentucky (22.6%), S. Idikan (15.1%) and other
serovars. This is the first report of isolation of S. Idikan from India. Thirty-two (60.4%) isolates were pan susceptible to
all drugs tested. Percentage distribution of resistant NTS against antimicrobials were: nalidixic acid (24.5%), ampicillin
(17%), amoxicillin (17%), streptomycin (15.1%), tetracycline (11.3%), chloramphenicol (9.4%), co-trimoxazole (9.4%) and
ciprofloxacin (5.7%). Only one isolate (S. Senftenberg) was resistant to both fluroquinolones and third generation cephalosporins.
Multidrug resistance (≥3 classes of antimicrobials) was observed in 13.2% NTS isolates.
Conclusion: High prevalence of NTS in poultry and meat samples in Kolkata poses a potential risk of foodborne disease
in humans. J Microbiol Infect Dis 2016;6(3): 113-120

Keywords

Non-typhoidal Salmonella, antimicrobial resistance, animals, poultry, food samples

References

• 1. Hohmann EL. Nontyphoidal Salmonellosis. Clin Infect Dis 2001; 32: 263-269.
• 2. Centers of Disease Control and Prevention (CDC). National Antimicrobial Resistance Monitoring System-Enteric Bacteria (NARMS): 2011 Human Isolates Final Report. Atlanta, GA: U.S. Department of Health and Human Services, 2013.
• 3. Suresh T, Hatha AA, Sreenivasan D, Sangeetha N, Lashmanaperumalsamy P. Prevalence and antimicrobial resistance of Salmonella Enteritidis and other Salmonellas in the eggs and egg-storing trays from retail markets of Coimbatore, South India. Food Microbiol 2006; 23: 294-299.
• 4. Esaki H, Morioka A, Ishihara K, et al. Antimicrobial susceptibility of Salmonella isolated from cattle, swine and poultry (2001-2002): report from the Japanese Veterinary Antimicrobial Resistance Monitoring Program. J Antimicrob Chemother 2004; 53: 266-270.
• 5. Yu T, Jiang X, Zhou Q, Wu J, Wu Z. Antimicrobial resistance, class 1 integrons, and horizontal transfer in Salmonella isolated from retail food in Henan, China. J Infect Dev Ctries 2014; 8: 705-711.
• 6. Mihaiu L, Lapusan A, Tanasuica R, et al. First study of Salmonella in meat in Romania. J Infect Dev Ctries 2014; 8: 50-58.
• 7. Murugkar HV, Rahman H, Kumar A, Bhattacharyya D. Isolation, phage typing and antibiogram of Salmonella from man & animals in northeastern India. Indian J Med Res 2005; 122: 237-242.
• 8. Moon AH. Studies on: Prevalence and antibiogram of Salmonella species of polluted meat origin. Asiatic J Biotech Res 2011; 2: 447-453.
• 9. Selvaraj R, Das R, Ganguly S, Ganguli M, Dhanalakshmi S, Mukhopadhayay SK. Characterization and antibiogram of Salmonella spp. from poultry specimens. J Microbiol Antimicrob 2010; 2: 123-126.
• 10. Selvaraj R, Das R, Ganguly S, Mukhopadhayay SK. Molecular characterization of Salmonella spp. isolated and identified from chicken samples. Int J Pharm Res Bio-Sci 2014; 3: 507-517.
• 11. World Health Organization (WHO). Global Foodborne Infections Network. Laboratory Protocol: “Isolation of Salmonella spp. from food and animal feaces” (5th Edition), 2010. (http://www.antimicrobialresistance.dk/data/images/protocols/ isolation_of_salm_220610.pdf)
• 12. World Health Organization (WHO). Global Foodborne Infections Network. Laboratory Protocol: “Biochemical Identification of Salmonella and Shigella using an abbreviated panel of tests” (3rd Edition), 2010. (http://www.antimicrobialresistance.dk/data/images/protocols/ gfn_biochem_final.pdf)
• 13. Guibourdenche M, Roggentin P, Mikoleit M, et al. Supplement 2003-2007 (No. 47) to the White-Kauffmann-Le Minor scheme. Res Microbiol 2010; 161: 26-29.
• 14. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement, M100-S24. Wayne, PA, 2014.
• 15. Chiu LH, Chiu CH, Horn YM, et al. Characterization of 13 multi-drug resistant Salmonella serovars from different broiler chickens associated with those of human isolates. BMC Microbiol 2010; 10: 86.
• 16. Addis Z, Kebede N, Worku Z, Gezahegn H, Yirsaw A, Kassa T. Prevalence and antimicrobial resistance of Salmonella isolated from lactating cows and in contact humans in dairy farms of Addis Ababa: a cross sectional study. BMC Infect Dis 2011; 11: 222.
• 17. White DG, Zhao S, Sudler R, et al. The isolation of antibioticresistant Salmonella from retail ground meats. N Engl J Med 2001; 345: 1147-1154.
• 18. Kaushik P, Anjay, Kumari S, Bharti S K, Dayal S. Isolation and prevalence of Salmonella from chicken meat and cattle milk collected from local markets of Patna, India. Vet World 2014; 7: 62-65.
• 19. Kshirsagar DP, Singh S, Brahmbhatt MN, Nayak JB. Isolation and molecular characterization of virulence-associated genes of Salmonella from buffalo meat samples in western region of India. IsrJ Vet Med 2014; 69: 228-233.
• 20. Tabo DA, Diguimbaye CD, Granier SA, et al. Prevalence and antimicrobial resistance of non-typhoidal Salmonella serotypes isolated from laying hens and broiler chicken farms in N’Djamena, Chad. Vet Microbiol 2013; 166: 293-298.
• 21. Singh BR, Singh M, Singh P, Babu N, Chandra M, Agarwal RK. Prevalence of multidrug-resistant Salmonella on readyto-eat betel leaves (Paan) and in water used for soaking betel leaves in North Indian cities. J Food Prot 2006; 69: 288-292.
• 22. Food and Drug Administration (FDA). National Antimicrobial Resistance Monitoring System – Enteric Bacteria (NARMS): 2011 Executive Report. Rockville, MD: U.S. Department of Health and Human Services, 2013. (http://www.fda.gov/ downloads/ AnimalVeterinary/ SafetyHealth/ AntimicrobialResistance/ NationalAntimicrobialResistanceMonitoringSystem/ UCM407962.pdf)
• 23. Barua H, Biswas PK, Talukder KA, Olsen KE, Christensen JP. Poultry as a possible source of non-typhoidal Salmonella enterica serovars in humans in Bangladesh. Vet Microbiol 2014; 168: 372-380.
• 24. Menezes GA, Khan MA, Harish BN, et al. Molecular characterization of antimicrobial resistance in non-typhoidal Salmonellae associated with systemic manifestations from India. J Med Micobiol 2010; 59:1477-1483.
• 25. Su LH, Teng WS, Chen CL, et al. Increasing ceftriaxone resistance in salmonellae, Taiwan. Emerg Infect Dis 2011; 17: 1086-1090.
• 26. Cheung TK, Chu YW, Chu MY, Ma CH, Yung RW, Kam KM. Plasmid-mediated resistance to ciprofloxacin and cefotaxime in clinical isolates of Salmonella enterica serotype Enteritidis in Hong Kong. J Antimicrob Chemother 2005; 56:586-589.
• 27. Wong MH, Yan M, Chan EW, Biao K, Chen S. Emergence of clinical Salmonella enterica serovar Typhimurium isolates with concurrent resistance to ciprofloxacin, ceftriaxone, and azithromycin. Antimicrob Agents Chemother 2014; 58: 3752- 3756.