Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders

Year 2019, Volume: 09 Issue: 03, 116 - 124, 15.09.2019

Radhakrishnan Selvi Perumal Venkatachalam Arcot Rekha K. Dharaniya S. Ayesha R. Selvaraj Shanthi Vijayaraghavan

https://doi.org/10.5799/jmid.614201

Abstract

ABSTRACT

Objectives: Helicobacter pylori, infection carryout a substantial role in the
pathogenesis of gastric disorders. The pathogenic effect is shown to be
associated with host bacterial interplay and Vac A and Cag A are
recognized as imperative virulence determinants. A variety of carcinogenic
pathways are triggered by H. pylori
and CCL20 and β-actin are responsible for cellular progress and upregulation of
these genes leads to metaplasia, dysplasia and gastric adenocarcinoma.
Therefore, it is of interest to investigate the association of Cag A, Vac A in the upregulation of CCL20
and β-actin expression and
progression towards gastric disorders.

Methods:
Blood and gastric biopsy samples of chronic gastritis subjects (n=100) were
collected to identify the incidence of H.
pylori by ELISA and Rapid urease test. The samples positive for both RUT
and ELISA were subjected to RT-PCR for expression studies.

Results:
Among the study subjects, 36% and 58% showed positive result for RUT and ELISA
of which 29% (p=0.0005) showed positive result for both RUT and ELISA. For
expression studies, 48% and 90% showed higher significant (p=0.0005) expression
levels of Cag A and Vac A m2. 10% (p=0.023) showed a
significant expression levels of Vac A
m1 region. Likewise 31% and 90% (p=0.0005) of patients showed higher
significant expression levels of CCL20
and β-actin.

Conclusion: Thus obtained results
illustrated that H. pylori excites
the production of Cag A, Vac A, CCL20 and β-actin and
expression of these factors signify a promising biomarker in the early
diagnosis of the infection and in analyzing the progression towards gastric
disorders. J Microbiol Infect Dis 2019; 9(3):116-124.

Keywords

Helicobacter pylori, CCL20, β-actin, RT- PCR, gastric disorders

References

• 1. Atherton JC. The pathogenesis of Helicobacter pylori induced gastro-duodenal diseases. Ann Review of Pathology 2006; 1:63-96. 2. Ito Y, Azuma T, Ito S et al. Analysis and typing of the Vac A Gene from Cag A-Positive Strains of Helicobacter pylori isolated in Japan. J Clin Microbiol 1997; 35:1710-1714. 3. Correa P. Human gastric carcinogenesis: a multistep and multifactorial process--First American Cancer Society Award Lecture on Cancer Epidemiology and Prevention. Cancer Res 1992;52:6735-6740. 4. Blaser MJ. Linking Helicobacter pylori to gastric cancer. Nature Medicine 2000;6:376-377. 5. Peek RM, Blaser MJ. Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nature Rev Cancer 2002;2:28-37. 6. Ernst PB, Peura DA, Crowe SE. The translation of Helicobacter pylori basic research to patient care. Gastroenterology 2006;130:188-206. 7. Blaser MJ, Perez-Perez GI, Kleanthous H, et al. Infection with Helicobacter pylori strains possessing Cag A associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res 1995; 55:2111-2115. 8. Nagy TA, Frey MR, Yan F, Israel DA, Polk DB, Peek RM Jr. Helicobacter pylori regulates cellular migration and apoptosis by activation of phosphatidylinositol 3-kinase signaling. J Infect Dis 2009; 199:641-651. 9. Ogiwara H, Sugimoto M, Ohno T et al. Role of deletion located between the intermediate and middle regions of the Helicobacter pylori Vac A gene in cases of gastroduodenal diseases. J Clin Microbiol 2009; 47:3493-3500. 10. Noach LA, Bosma NB, Jansen J, Hoek FJ, Van Deventer SJ, Tytgat GN. Mucosal tumor necrosis factor-alpha, interleukin-1 beta, and interleukin-8 production in patients with Helicobacter pylori infection. Scand J Gastroenterol 1994; 29:425-429. 11. Liao F, Rabin RL, Smith CS, Sharma G, Nutman TB, Farber JM. CC-chemokine receptor 6 is expressed on diverse memory subsets of T cells and determines responsiveness to macrophage inflammatory protein 3α. J Immun 1999; 162:186-194. 12. Wu YY, Tsai HF, Lin WC, et al. Upregulation of CCL20 and Recruitment of CCR6+ Gastric Infiltrating Lymphocytes in Helicobacter pylori Gastritis. Infect Immun 2007; 75:4357-4363. 13. Khan SA, Tyagi M, Sharma AK et al. Cell-type specificity of β-actin expression and its clinicopathological correlation in gastric adenocarcinoma. W J Gastroenterol 2014; 20:12202-12211. 14. Xu J, Zhang Z, Chen J, Liu F, Bai L. Over expression of β-actin is closely associated with metastasis of gastric cancer. Hepatogastroenterology 2013; 60:620-623. 15. Elitsur Y, Hill I, Lichtman SN, Rosenberg AJ. Prospective Comparison of Rapid Urease Tests (PyloriTek, CLO Test) for the Diagnosis of Helicobacter pylori Infection in Symptomatic Children: A Pediatric Multicenter Study. Americ J Gastroenterol 1998; 93:217-219. 16. Ping-I Hsu. Application of Serology in the Diagnosis of Helicobacter pylori Infection in Patients with Atrophic Gastritis. J Chine Med Associat 2010; 73:561-562. 17. Hung HH, Chen TS, Lin HC. Immunoglobulin G Antibody against Helicobacter pylori is an accurate Test for atrophic gastritis. Journal of the Chinese Medical Association 2010; 73:355-359. 18. Cutler AF, Prasad VM, Santogade P. Four-year trends in Helicobacter pylori IgG serology following successful eradication. The American Journal of Medicine 1998; 105:18-20. 19. Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 1987; 162:156-159. 20. Wang J, Wang WH, Li J, Liu FX. Celecoxib inhibits Helicobacter pylori colonization-related factors. World Journal of Gastroenterology 2010;16:846-853. 21. Qiang He, Jian-Ping Wang, Michael Osato, Lawrence B Lachman. Real-Time Quantitative PCR for detection of Helicobacter pylori. J Clin Microbiol 2002; 40:3720-3728. 22. Peek RM Jr, Miller GG, Tham KT et al. Detection of Helicobacter pylori Gene Expression in Human Gastric Mucosa. J Clin Microbiol 1995; 33:28-32. 23. Wroblewski LE, Peek RM Jr, Wilson KT. Helicobacter pylori and Gastric Cancer: Factors that Modulate Disease Risk. Clinal Microbiol Rev 2010; 23:713-739. 24. Covacci A, Censini S, Bugnoli M et al. Molecular characterization of the 128-kDa immune dominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci U S A; 1993; 90:5791–5795. 25. Cover TL, Dooley CP, Blaser MJ. Characterization and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infection and Immunity 1990; 58:603-610. 26. Crabtree JE, Taylor JD, Wyatt JI et al. Mucosal IgA recognition of Helicobacter pylori 120 kDa protein, peptic ulceration, and gastric pathology. Lancet 1991; 338:332-335. 27. Foegeding NJ, Caston RR, McClain MS, Ohi MD, Cover TL. An overview of Helicobacter pylori Vac A Toxin Biology. Toxins 2016; 8:173. 28. Xu J, Zhang Z, Chen J, Liu F, Bai L. Over expression of β-actin is closely associated with metastasis of gastric cancer. Hepatogastroenterology 2013; 60:620-623. 29. Yoshida A, Isomoto H, Hisatsune J et al. Enhanced expression of CCL20 in human Helicobacter pylori associated Gastritis. Clin Immun 2009; 130:290–297.