Year 2019, Volume 09 , Issue 03, Pages 116 - 124 2019-09-15

Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders

Radhakrishnan Selvi [1] , Perumal Venkatachalam [2] , Arcot Rekha [3] , K. Dharaniya [4] , S. Ayesha [5] , R. Selvaraj [6] , Shanthi Vijayaraghavan [7]


ABSTRACT

Objectives: Helicobacter pylori, infection carryout a substantial role in the pathogenesis of gastric disorders. The pathogenic effect is shown to be associated with host bacterial interplay and Vac A and Cag A are recognized as imperative virulence determinants. A variety of carcinogenic pathways are triggered by H. pylori and CCL20 and β-actin are responsible for cellular progress and upregulation of these genes leads to metaplasia, dysplasia and gastric adenocarcinoma. Therefore, it is of interest to investigate the association of Cag A, Vac A in the upregulation of CCL20 and β-actin expression and progression towards gastric disorders.

Methods: Blood and gastric biopsy samples of chronic gastritis subjects (n=100) were collected to identify the incidence of H. pylori by ELISA and Rapid urease test. The samples positive for both RUT and ELISA were subjected to RT-PCR for expression studies.

Results: Among the study subjects, 36% and 58% showed positive result for RUT and ELISA of which 29% (p=0.0005) showed positive result for both RUT and ELISA. For expression studies, 48% and 90% showed higher significant (p=0.0005) expression levels of Cag A and Vac A m2. 10% (p=0.023) showed a significant expression levels of Vac A m1 region. Likewise 31% and 90% (p=0.0005) of patients showed higher significant expression levels of CCL20 and β-actin.

Conclusion: Thus obtained results illustrated that H. pylori excites the production of Cag A, Vac A, CCL20 and β-actin and expression of these factors signify a promising biomarker in the early diagnosis of the infection and in analyzing the progression towards gastric disorders. J Microbiol Infect Dis 2019; 9(3):116-124.
Helicobacter pylori, CCL20, β-actin, RT- PCR, gastric disorders
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Primary Language en
Journal Section Research Article
Authors

Author: Radhakrishnan Selvi (Primary Author)
Institution: Technology and Research, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, India
Country: India


Author: Perumal Venkatachalam

Author: Arcot Rekha

Author: K. Dharaniya

Author: S. Ayesha

Author: R. Selvaraj

Author: Shanthi Vijayaraghavan

Dates

Publication Date : September 15, 2019

Bibtex @research article { jmid614201, journal = {Journal of Microbiology and Infectious Diseases}, issn = {2146-3158}, eissn = {2146-9369}, address = {}, publisher = {Sağlık Araştırmaları Derneği}, year = {2019}, volume = {09}, pages = {116 - 124}, doi = {10.5799/jmid.614201}, title = {Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders}, key = {cite}, author = {Selvi, Radhakrishnan and Venkatachalam, Perumal and Rekha, Arcot and Dharaniya, K. and Ayesha, S. and Selvaraj, R. and Vijayaraghavan, Shanthi} }
APA Selvi, R , Venkatachalam, P , Rekha, A , Dharaniya, K , Ayesha, S , Selvaraj, R , Vijayaraghavan, S . (2019). Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders. Journal of Microbiology and Infectious Diseases , 09 (03) , 116-124 . DOI: 10.5799/jmid.614201
MLA Selvi, R , Venkatachalam, P , Rekha, A , Dharaniya, K , Ayesha, S , Selvaraj, R , Vijayaraghavan, S . "Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders". Journal of Microbiology and Infectious Diseases 09 (2019 ): 116-124 <https://dergipark.org.tr/en/pub/jmid/issue/48485/614201>
Chicago Selvi, R , Venkatachalam, P , Rekha, A , Dharaniya, K , Ayesha, S , Selvaraj, R , Vijayaraghavan, S . "Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders". Journal of Microbiology and Infectious Diseases 09 (2019 ): 116-124
RIS TY - JOUR T1 - Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders AU - Radhakrishnan Selvi , Perumal Venkatachalam , Arcot Rekha , K. Dharaniya , S. Ayesha , R. Selvaraj , Shanthi Vijayaraghavan Y1 - 2019 PY - 2019 N1 - doi: 10.5799/jmid.614201 DO - 10.5799/jmid.614201 T2 - Journal of Microbiology and Infectious Diseases JF - Journal JO - JOR SP - 116 EP - 124 VL - 09 IS - 03 SN - 2146-3158-2146-9369 M3 - doi: 10.5799/jmid.614201 UR - https://doi.org/10.5799/jmid.614201 Y2 - 2019 ER -
EndNote %0 Journal of Microbiology and Infectious Diseases Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders %A Radhakrishnan Selvi , Perumal Venkatachalam , Arcot Rekha , K. Dharaniya , S. Ayesha , R. Selvaraj , Shanthi Vijayaraghavan %T Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders %D 2019 %J Journal of Microbiology and Infectious Diseases %P 2146-3158-2146-9369 %V 09 %N 03 %R doi: 10.5799/jmid.614201 %U 10.5799/jmid.614201
ISNAD Selvi, Radhakrishnan , Venkatachalam, Perumal , Rekha, Arcot , Dharaniya, K. , Ayesha, S. , Selvaraj, R. , Vijayaraghavan, Shanthi . "Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders". Journal of Microbiology and Infectious Diseases 09 / 03 (September 2019): 116-124 . https://doi.org/10.5799/jmid.614201
AMA Selvi R , Venkatachalam P , Rekha A , Dharaniya K , Ayesha S , Selvaraj R , Vijayaraghavan S . Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders. J Microbil Infect Dis. 2019; 09(03): 116-124.
Vancouver Selvi R , Venkatachalam P , Rekha A , Dharaniya K , Ayesha S , Selvaraj R , Vijayaraghavan S . Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders. Journal of Microbiology and Infectious Diseases. 2019; 09(03): 124-116.