Helicobacter pylori Virulent Genes in the Upregulation of CCL20 and β-actin Expression and Progression towards Gastric Disorders

Yıl 2019, Cilt: 09 Sayı: 03, 116 - 124, 15.09.2019

Radhakrishnan Selvi Perumal Venkatachalam Arcot Rekha K. Dharaniya S. Ayesha R. Selvaraj Shanthi Vijayaraghavan

https://doi.org/10.5799/jmid.614201

Öz

ABSTRACT

Objectives: Helicobacter pylori, infection carryout a substantial role in the
pathogenesis of gastric disorders. The pathogenic effect is shown to be
associated with host bacterial interplay and Vac A and Cag A are
recognized as imperative virulence determinants. A variety of carcinogenic
pathways are triggered by H. pylori
and CCL20 and β-actin are responsible for cellular progress and upregulation of
these genes leads to metaplasia, dysplasia and gastric adenocarcinoma.
Therefore, it is of interest to investigate the association of Cag A, Vac A in the upregulation of CCL20
and β-actin expression and
progression towards gastric disorders.

Methods:
Blood and gastric biopsy samples of chronic gastritis subjects (n=100) were
collected to identify the incidence of H.
pylori by ELISA and Rapid urease test. The samples positive for both RUT
and ELISA were subjected to RT-PCR for expression studies.

Results:
Among the study subjects, 36% and 58% showed positive result for RUT and ELISA
of which 29% (p=0.0005) showed positive result for both RUT and ELISA. For
expression studies, 48% and 90% showed higher significant (p=0.0005) expression
levels of Cag A and Vac A m2. 10% (p=0.023) showed a
significant expression levels of Vac A
m1 region. Likewise 31% and 90% (p=0.0005) of patients showed higher
significant expression levels of CCL20
and β-actin.

Conclusion: Thus obtained results
illustrated that H. pylori excites
the production of Cag A, Vac A, CCL20 and β-actin and
expression of these factors signify a promising biomarker in the early
diagnosis of the infection and in analyzing the progression towards gastric
disorders. J Microbiol Infect Dis 2019; 9(3):116-124.

Anahtar Kelimeler

Helicobacter pylori, CCL20, β-actin, RT- PCR, gastric disorders

Kaynakça

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