İmidakloprid tayini için moleküler baskılanmış polimer kullanılarak yüzey plasmon rezonans nanosensör geliştirilmesi

Abstract

İmidakloprid (IMI), çeşitli mahsullerde etkili haşere kontrolü ve tohum ve toprak zararlılarının yönetimi için yaygın olarak kullanılan bir neonikotinoid insektisittir. Bu çalışmada, IMI tespiti için bir yüzey plazmon rezonansı (SPR) nanosensörü geliştirmeye odaklandık. İlk adımımız, SPR çipinin altın yüzeyini modifiye etmekti. Bu, bir monokatman oluşturmak için allilmerkaptanın kendi kendine birleşmesi yoluyla gerçekleştirildi ve böylece çipin yüzeyine polimerleşebilir çift bağlar eklendi. Bundan sonra, SPR çipinin altın yüzeyine IMI baskılı poli(2-hidroksietilmetakrilat-metakriloilamidoglutamikasit) [p(HEMA-MAGA)] filmi tasarlandı. Hem modifiye edilmemiş hem de IMI baskılı p(HEMA-MAGA) yüzeyleri, atomik kuvvet mikroskopisi (AFM), Fourier dönüşümlü kızılötesi (FTIR) spektroskopisi ve elipsometri dahil olmak üzere bazı analitik teknikler kullanılarak karakterize edildi. Geliştirilen SPR nanosensörü, 1,0 ng L-1 ile 10,0 ng L-1 arasında doğrusal bir algılama aralığı sergiledi ve 0,33 ng L-1 kadar düşük bir algılama sınırına (LOD) ulaştı ve son olarak yüksek geri kazanım oranıyla içme suyu örneklerine uygulandı.

Keywords

• İmidakloprid
• Yüzey Plazmon Rezonansı
• moleküler baskılanmış polimerler
• uygulama

Surface Plasmon Resonance Nanosensor Development for Imidacloprid Detection Using Molecularly Imprinting Polymer

Abstract

Imidacloprid (IMI) is an extensively used neonicotinoid insecticide, widely applied for effective pest control in various crops, as well as for the management of seed and soil pests. Monitoring IMI levels can provide important contamination risks, inform regulatory decisions regarding pesticide use, and guide strategies aimed at mitigating adverse environmental consequences. In this study, we focused on developing a surface plasmon resonance (SPR) nanosensor for IMI detection. Our initial step involved modifying the gold surface of the SPR chip. This was achieved through the self-assembly of allylmercaptane to form a monolayer, thus introducing polymerizable double bonds onto the chip's surface. After that, IMI imprinted poly(2-hydroxyethylmethacrylate–methacryloylamidoglutamicacid) [p(HEMA–MAGA)] film was designed on gold surface of SPR chip. The surfaces, both non-modified and IMI-imprinted p(HEMA–MAGA), were characterized using some analytical techniques, including atomic force microscopy (AFM), fourier transform infrared (FTIR) spectroscopy, and ellipsometry. The developed SPR nanosensor demonstrated a linear detection range between 1.0 ng L-1 and 10.0 ng L-1 and accomplished a limit of detection (LOD) as low as 0.33 ng L-1 and finally applied to the drinking water samples with high recovery.

Keywords

• Imidacloprid
• Surface Plasmon Resonance
• Molecularly imprinting polymers
• Application

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