Farklı Çözücülerin Gongolaria barbata'nın (Phaeophyceae) Antioksidan Özellikleri Üzerine Etkisi

Year 2021, , 197 - 201, 29.12.2021

Melis Yılmaz Gülen Türker , İlknur Ak

https://doi.org/10.46384/jmsf.1021387

Cited By: 1

Abstract

Bu çalışmada, Gongolaria barbata'dan elde edilen alg ekstraktlarının antioksidan aktiviteleri, toplam fenolik ve flavonoid içerikleri sırasıyla farklı çözücüler kullanılarak incelenmiştir: etil asetat (AcOEt), su(W) ve fosfat tamponu (PH). Sonuçlara göre EtOH, W ve PB çözücüleri, sırasıyla test edilen iki ticari antioksidan olan BHT ve Vitamin C'den önemli ölçüde daha yüksek olduğu ve düşük IC50 değerleri ile orta düzeyde antioksidan potansiyeli gösterdiği kaydedildi. En yüksek toplam flavonoid içeriği W (6.91±0,09 mg/g Ext.) olarak tespit edildi ve PB en yüksek fenolik içeriği (2.29±0.01 mg GAE/g ext.) gösterdi. Klorofil a ve toplam karotenoid değerleri sırasıyla 40,5±1,20 ve 29,13±1,12 (mg/g DW) olarak ölçüldü. Fikoeritrin 0.002±0.001 (mg/g DW) olarak ölçülürken, fikosiyanin içeriği 0.04 ±0.01 (mg/g DW) olarak bulundu. Çalışmamızın sonucuna göre, Gongolaria barbata’nın antioksidan içerikleri, toplam fenolik ve flavonoid içerikleri incelendiğinde, fosfat tamponunun etanolün yerine kullanılabilecek bir çözücü olduğu belirlenmiştir.

Keywords

Antioksidan özellikleri, Fenolik, Flavonoid, Gongolaria barbata, Kahverengi alg

The Effect of Different Solvents on Antioxidant Properties of Gongolaria barbata (Phaeophyceae)

Abstract

In this study, the antioxidant activities, total polyphenolic contents of algal extracts from Gongolaria barbata were examined by using ethyl acetate (AcOEt), water(W), and phosphate buffer (PH) as solvents. According to the results, EtOH, W, and PB showed medium antioxidant potential with low IC50 values. The highest total flavonoid content was detected in W (6.91±0.09 mg/g Ext., and PB showed the maximum phenolic content (2.29±0.01 mg GAE/g ext.). The chlorophyll a and total carotenoid contents were detected as 40.5±1.20 and 29.13±1.12 (mg/g DW), respectively. Phycoerythrin was 0.002±0.001 (mg/g DW), while phycocyanin content was found as0.04 ±0.01 (mg/g DW). Our results show that PB could be used as an extraction solvent for determining the antioxidant properties of G. barbata instead of ethanol.

Keywords

Antioxidant properties, brown seaweed, flavonoids, Gongolaria barbata, phenolics.

References

• Ak, İ., & Türker, G. (2019). Antioxidant Activities of Eucheuma sp. (Rhodophyceae) and Laminaria sp. (Phaeophyceae). Turkish Journal of Agriculture - Food Science and Technology, 7, 154-159. doi: 10.24925/turjaf.v7isp1.154-159.2791
• Ak, İ., & Yücesan, M. (2012). Effect of light intensity on the pigment composition of Gracilaria verrucosa (Rhodophyta). Fresenius Environmental Bulletin 21 (2), 337-342.
• Banwo, K., Olojede, A. O., Adesulu-Dahunsi, A. T., Verma, D. K., Thakur, M., Tripathy, S., Singh, S., Patel, A. R., Gupta, A. K., Aguilar, C. N., & Utama, G. L. (2021). Functional importance of bioactive compounds of foods with Potential Health Benefits: A review on recent trends. Food Bioscience, 43, 101320. doi: 10.1016/j.fbio.2021.101320
• Beer, S., & Eshel, A. (1985). Determining phycoerythrin and phycocyanin concentrations in aqueous crude extracts of red algae. Marine and Freshwater Research, 36 (6), 785-792.
• Bizzaro, G., Vatland, A.K., & Pampanin, D.M. (2022). The one-health approach in seaweed food production. Environment International, 158:106948. doi: 10.1016/j.envint.2021.106948
• Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28(1), 25-30. doi: 10.1016/S0023-6438(95)80008-5
• Büchel, C. (2020). Light harvesting complexes in chlorophyll c-containing algae. Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1861(4), 148027. doi: 10.1016/j.bbabio.2019.05.003
• Cirik, Ş., Şen, E., & Ak, İ. (2010). Brown algae Cystoseira barbata (Stackhouse) C. Agardh culture and changes in it chemical composition. Journal of fisheriessciences.com, 4(4), 354-361. doi:10.3153/jfscom.2010038
• Devi, A., Dwibedi, V., & Khan, Z. A. (2021). Natural antioxidants in new age-related diseases. Revista Brasileira de Farmacognosia, 31(4), 387-407. doi:10.1007/s43450-021-00175-0
• Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., & Vidal, N. (2006). Antioxidant activity of some algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654-660. doi:https://doi.org/10.1016/j.foodchem.2005.04.028
• Guiry, M. D., & Guiry, G. M. (2021). AlgaeBase. Access date: 07 October 2021, https://www.algaebase.org
• Ismail, G. A. (2017). Biochemical composition of some Egyptian seaweeds with potent nutritive and antioxidant properties. Food Science and Technology, 37(2), 294-302. doi: 10.1590/1678-457x.20316
• Jeffrey, S. W., & Humphrey, G. F. (1975). New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton. Biochemie und Physiologie der Pflanzen, 167(2), 191-194. doi: 10.1016/S0015-3796(17)30778-3
• Jégou, C., Connan, S., Bihannic, I., Cérantola, S., Guérard, F., & Stiger-Pouvreau, V. (2021). Phlorotannin and pigment content of native canopy-forming sargassaceae species living in intertidal rockpools in Brittany (France): Any relationship with their vertical distribution and phenology? Marine Drugs, 19(9), 504-524. doi: 10.3390/md19090504
• Koru, E., Yilmaz, M., Turker, G., & Ak, I. (2021). Antioxidant properties and element levels of two commercial Arthrospira strains. Phycologia. 60(sup1), 132-132. doi: 10.1080/00318884.2021.1922050
• Ktari, L., Chebil Ajjabi, L., De Clerck, O., Gómez Pinchetti, J. L., & Rebours, C. (2021). Seaweeds as a promising resource for blue economy development in Tunisia: current state, opportunities, and challenges. Journal of Applied Phycology. doi:10.1007/s10811-021-02579-w
• Mildenberger, J., Stangeland, J.K., Rebours, C. (2022). Antioxidative activities, phenolic compounds and marine food allergens in the macroalgae Saccharina latissima produced in integrated multi-trophic aquaculture systems. Aquaculture,546(15): 737386. doi: 10.1016/j.aquaculture.2021.737386
• Osório, C., Machado, S., Peixoto, J., Bessada, S., Pimentel, F. B., C. Alves, R., & Oliveira, M. B. P. P. (2020). Pigments content (chlorophylls, fucoxanthin and phycobiliproteins) of different commercial dried algae. Separations, 7(2), 33. doi: 10.3390/separations7020033
• Öztaşkent, C. & Ak, İ. (2021). Effect of LED light sources on the growth and chemical composition of brown seaweed Treptacantha barbata. Aquacult Int 29: 193–205 doi: 10.1007/s10499-020-00619-9
• Quettier-Deleu, C., Gressier, B., Vasseur, J., Dine, T., Brunet, C., Luyckx, M., Cazin, M., Cazin, J.-C., Bailleul, F., & Trotin, F. (2000). Phenolic compounds and antioxidant activities of buckwheat (Fagopyrum esculentum Moench) hulls and flour. Journal of Ethnopharmacology, 72(1), 35-42. doi:https://doi.org/10.1016/S0378-8741(00)00196-
• Ślusarczyk, J., Adamska, E., & Czerwik-Marcinkowska, J. (2021). Fungi and algae as sources of medicinal and other biologically active compounds: a review. Nutrients, 13(9), 3178. doi: 10.3390/nu13093178
• Shubina, V.S., Kozina, V.I., & Shatalin, Y.V. (2021). Comparison of antioxidant properties of a conjugate of taxifolin with glyoxylic acid and selected flavonoids. antioxidants, 10:1262. doi: 10.3390/antiox10081262
• Wang, H.-M. D., Li, X.-C., Lee, D.-J., & Chang, J.-S. (2017). Potential biomedical applications of marine algae. Bioresource Technology, 244, 1407-1415. doi: 10.1016/j.biortech.2017.05.198
• Zhu, X., Healy, L.E.i Sevindik, O., Sun, D., Selli, S. Kelebek, H., Tiwari, B.K. (2022). Impacts of novel blanching treatments combined with commercial drying methods on the physicochemical properties of Irish brown seaweed Alaria esculenta. Food Chemistry, 369(1): 130949. doi: 10.1016/j.foodchem.2021.130949